In an optical measurement device, in a first process, a reference member is irradiated with excitation light, light for a calibration process including scattered light associated with the excitation light from a reference member is detected as detection light, a calibration signal corresponding to the light for the calibration process is designated as a detection signal, and a calibration process for removing a signal component corresponding to the scattered light from the detection signal in a second process is performed. Also, in the optical measurement device, in the second process, a sample is irradiated with the excitation light, measurement target light including fluorescence generated from the sample and light scattered from the sample irradiated with the excitation light is detected as detection light, and a signal component corresponding to the scattered light from a measurement signal.

A suitable fluorescence separation method is set according to an object to be measured. An information processing device according to an embodiment includes a separation unit (14) that calculates, from a fluorescence signal measured from a biological sample labeled with one or more fluorescent dyes, fluorescence intensities of one or more fluorescent beams and an autofluorescent beam emitted from the one or more fluorescent dyes and the biological sample, respectively, by an arithmetic operation using a least squares method using a fluorescence spectrum reference of each of the fluorescent dyes and an autofluorescence spectrum of the biological sample. In the arithmetic operation using the least squares method, an upper limit value and a lower limit value of the fluorescence intensity are set for each of the one or more fluorescent beams and the autofluorescent beam.

A method of measuring optical properties of a specimen includes generating illumination light at a plurality of illumination wavelengths and, for each of the plurality of illumination wavelengths, directing the illumination light to impinge on the specimen, collecting sample light passing through the specimen, and detecting the collected sample light using a polarization state analyzer to form a set of polarization channels. The method also includes receiving a calibration tensor, converting the set of polarization channels for each of the illumination wavelengths into Stokes parameter maps using the calibration tensor, denoising the Stokes parameter maps, and deconvolving the Stokes parameter maps to provide density, anisotropy, and orientation measurements of the specimen. The method can multiplex intrinsic density, anisotropy, and orientation measurements of the specimen and density, anisotropy, and orientation measurements of labeled fluorescent molecules.

A wood stove monitoring and control device can include a mounting flange mountable to a chimney exhaust pipe of a wood stove. The device can include a ring removably mountable on top of the mounting flange, where the flange is suitably positioned vertically along the exhaust pipe so that the ring is positioned at least partially above an end of the exhaust pipe. The device includes an optical beam source disposed on the ring, and which generates and outputs an optical beam. The device includes an optical sensor positioned on the ring opposite the optical beam source to detect the optical beam output by the optical beam source as the optical beam passes through smoke exhausted by the wood stove through the exhaust pipe. The device can include a temperature probe disposed on the ring to measure a temperature of heat exhausted by the wood stove through the exhaust pipe.

A device may obtain a master beta coefficient of a master calibration model associated with a master instrument. The master beta coefficient may be at a grid of a target instrument. The device may perform constrained optimization of an objective function, in accordance with a set of constraints, in order to determine a pair of transferred beta coefficients. The constrained optimization may be performed based on an initial pair of transferred beta coefficients, the master beta coefficient, and spectra associated with a scouting set. The device may determine, based on the pair of transferred beta coefficients, a transferred beta coefficient. The device may determine a final transferred beta coefficient based on a set of transferred beta coefficients including the transferred beta coefficient. The final transferred beta coefficient may be associated with generating a transferred calibration model, corresponding to the master calibration model, for use by the target instrument.

A method for inspection of multiple features of patterned objects in the manufacture of electrical circuits, the method including performing defect detection on the patterned object, employing an optical defect detection machine (ODDM) and employing the ODDM to measure at least one of spatial coordinates and physical attributes of at least some of the multiple features.

A method of inspecting a wafer comprising measuring an intensity of an incident light and storing the measurement as stored incident light intensity, irradiating the incident light to the wafer, measuring an intensity of a reflected light from the wafer and storing the measurement as stored reflected light intensity, and correcting the stored reflected light intensity based on a difference between the stored incident light intensity and a reference intensity of a reference incident light.

A method of calibrating an optical detector includes affixing a calibration material to a first surface of the optical detector and calibrating one or more parameters of the optical detector using the calibration material.

A microplate reader simultaneously obtains Raman measurements from samples contained in non-adjacent wells. At least two Raman probes are positioned perpendicularly above or below the microplate to simultaneously acquire Raman spectra data of the non-adjacent liquid samples. Each probe is coupled to a laser and a spectrometer and includes a lens focusing laser light within the sample and collecting light from the sample for the spectrometer. The spectrometer may include a 2D imaging sensor (sCMOS or CCD) to image light from multiple probes simultaneously, spaced from one another to reduce crosstalk. A positioner moves the microplate plate or probes to acquire data from a different subset of non-adjacent samples, and may also vary laser focus within wells during data acquisition. Multiple fluorescence probes may simultaneously acquire fluorescence data from the same samples, or non-adjacent samples. Probes may be fiber-coupled and positioned within a reaction chamber of a liquid handling system.

A method to make it possible to obtain a value related to the amount of DD by FDP measurement. The method includes optically measuring a first calibration sample prepared from an FDP measurement reagent and a first calibrator containing D-dimer (DD) and having a first value relating to the ratio of the content of fibrin/fibrinogen degradation product FDP to the content of DD, acquiring first calculation data based on temporal change of optical information obtained by optical measurement of the first calibration measurement sample, performing optical measurement of a second calibration measurement sample prepared from FDP measurement reagent and a second calibrator containing DD and having a second value that is different from the first value related to the ratio of the content of FDP to the content of DD, acquiring second calculated data based on a temporal change in optical information obtained by optical measurement of the second calibration measurement sample, and acquiring calibration curve information indicating the relationship between the calculation data and the value relating to the amount of DD based on the first calculation data, the second calculation data, the first value, and the second value.