Methods and devices are disclosed for the tomographic imaging of a biological sample from almost all rotational perspectives in three-dimensional space and with multiple imaging modalities. A biological sample is positioned on an imaging stage that is capable of nearly full 360-degree rotation in at least one of two substantially orthogonal axes. Positioned about the stage is an X-ray imaging module enabling the recording of a series of images. A reflected light imaging module can also be positioned about the stage to enable recording of black and white or color white light images. A computer can use the images to construct three-dimensional models of the sample and to render images of the sample conveying information from one or more imaging channels.

A method for dark-field imaging includes acquiring dark-field image projections of an object with an imaging apparatus that includes an x-ray interferometer, applying a pressure wave having a predetermined frequency to the object for each acquired projection, wherein the predetermined frequency is different for each projection, and processing the acquired projections, thereby generating a 3D image of the object. In other words, the method corresponds to acoustically modulated X-ray dark field tomography. An imaging system (400) includes a scanner (401) configured for dark-field imaging, the scanner including: a source/detector pair (402/408) and a subject support (416), a pressure wave generator (420) configured to generate and transmit pressure waves having predetermined frequencies, and a console (424) that controls the scanner and the pressure wave generator to acquire at least two dark-field projection of an object with different pressure waves having different frequencies applied to the object.

The present invention is an X-ray system having a source-detector module, which includes X-ray sources and detectors, for scanning an object being inspected, a scan engine coupled to the source-detector module for collecting scan data from the source detector module, an image reconstruction engine coupled to the scan engine for converting the collected scan data into one or more X-ray images, and a scan controller coupled with at least one of the source detector module, the scan engine, and the image reconstruction engine optimize operations of the X-ray system.

The invention is a diagnostic which overlays quantum mechanical analysis to x-ray crystallography data from one or more proteins to assess and identify the real world conformation, protonation and solvent effects of one or more moieties in said protein. This “overlay” occurs by scoring and identifying the protomer/tautomer states of the moieties using quantum mechanical analysis. The diagnostic results of the present invention accurately identify protein-ligand binding, rendered as an output to a user of a computer in which the x-ray crystallography data is analysed with semi-empirical Hamiltonian quantum mechanics and

Methods, apparatus, and processes which use Extreme ultraviolet radiation (EUV) and/or soft X-ray wavelengths to read, image, edit, locate, identify, map, alter, delete, repair and sequence genes are described. An EUV scanning tool which allows high throughput genomic scanning of DNA, RNA and protein sequences is also described. A database which records characteristic absorption spectra of gene sequences is also described.

There is provided systems and methods for determining variability of cryo-EM protein structures from a set of cryo-electron microscope images. The method includes: performing iterative optimization, each optimization iteration including: determining the updated variability coordinates for individual images from the set of images using a current value of the variability components; determining the updated variability components for multiple images of the set of images, using the updated value of the variability coordinates, by solving a set of linear equations, the linear equations comprising a sum of weighted compositions of projection and back-projection operators, the equations are solved by arranging the equations into a block-diagonal matrix form.

A device (1) for detecting a concentration of predetermined particles, particularly viruses, in air (3) with organic and/or inorganic aerosol particles, has a supply unit (10), an imaging unit (20), an image acquisition unit (40) and an evaluation unit (50). The supply unit (10) binds the aerosol particles as particles in a fluid (4). The imaging unit (20) operates on the functional principle of a scanning electron microscope in order to generate an enlarged image of the particles contained in the fluid (4). The image acquisition unit (40) acquires and transmits the image. The evaluation unit (50) evaluates the particles depicted in the image. The evaluation unit (50) automatically detects morphological properties of the particles depicted in the image and compares the detected morphological properties with morphological properties of the predetermined particles. Through the comparison, it determines a proportion and/or number of predetermined particles in the image and the concentration of the predetermined particles in the air (3).

The invention is a diagnostic which overlays quantum mechanical analysis to x-ray crystallography or Cryo-EM data from one or more molecules, to assess and identify the real world conformation, protonation and solvent effects of one or more moieties in said molecule. This “overlay” occurs by scoring and identifying the protomer/tautomer or conformational states of the moieties using quantum mechanical analysis. The diagnostic results of the present invention accurately identify protein-ligand binding, rendered as an output to a user of a computer in which the x-ray crystallography or Cryo-EM data is analysed with semi-empirical Hamiltonian quantum mechanics.

A diagnostic support for a skin includes a radio-transparent structure that defines a folding surface of the skin and on which the skin may be stretched and consequently folded, thereby defining folded, mutually superimposed portions spaced apart from each other. The support may be used for radiographic inspection of a folded animal skin.

Provided are processes and materials for solving biological or structural information about proteins or other organic molecules. The processes capitalize on a rigid multimeric nanocage formed from self-assembling substructure proteins. The processes and materials allow for recognition and tight, optionally covalent, bonding of any protein molecule with a tag complementary to a capture sequence on the nanocage. The processes and materials may be used to obtain biological or structural information by cryo-electron microscopy and overcome prior limitations of target protein size or salt concentration.