The present disclosure relates to the field of a cabinet x-ray incorporating an x-ray tube, an x-ray detector, and a real-time camera, either high definition or standard resolution, for the production of organic and non-organic images. The computing device can receive video data from the camera and the x-ray detector and determines, based on the video data, an overlay of the captured x-ray image with the captured real-time image or display images adjacently i.e. Picture-In-Picture (PIP). In particular, the disclosure relates to a system and method with corresponding apparatus for capturing a real-time image simultaneously with the x-ray image allowing a cabinet x-ray unit to attain and optimize images with exact orientation of the 2 images.

A protein crystal comprising a first protein crystal having available space in the lattice, wherein a second protein crystal and a moiety can be accommodated in the available space in the lattice. The first and second proteins are co-expressed from one or more nucleic acid constructs. In a preferred embodiment, the first protein is the p21-activated kinase PAK4, the second protein is the PAK4 kinase inhibitor Inka1, and the moiety comprises a reporter molecule such as fluorescent proteins or tags and is fused to the iBox or iBox-C or Inka1. Preferably the crystal is formed in cellulo. Also provided is a fusion protein comprising the first protein and the second protein, wherein upon crystallisation the second protein fits within the available space in the lattice of the first protein, along with the moiety. Methods for producing the protein crystal are also disclosed.

An electron diffraction imaging system for imaging the three-dimensional structure of a single target molecule of a sample uses an electron source that emits a beam of electrons toward the sample, and a two-dimensional detector that detects electrons diffracted by the sample and generates an output indicative of their spatial distribution. A sample support is transparent to electrons in a region in which the sample is located, and is rotatable and translatable in at least two perpendicular directions. The electron beam has an operating energy between 5 keV and 30 keV, and beam optics block highly divergent electrons to limit the beam diameter to no more than three times the size of the sample molecule and provide a lateral coherence length of at least 15 nm. An adjustment system adjusts the sample support position in response to the detector output to center the target molecule in the beam.

Radiographic imaging system including: an x-ray transmission unit; an x-ray receiver unit; a plate made from a material opaque to x-rays and situated between the transmission unit and the receiver unit, the plate including at least four channels, each channel enabling a part of the x-rays emitted by the transmission unit to pass through the channel; and an image processing unit configured to determine the coordinates of the projected patterns and to calculate a position of the receiver unit from the coordinates of the projected patterns and from the coordinates of the channels.

A water stress detection method for tomatoes in a seedling stage based on micro-CT and polarization-hyperspectral imaging multi-feature fusion, comprising: using micro-CT to scan microscopic morphological features such as water stress stomata, spongy body, palisade tissue, cilia, vascular bundle, root volume, main root, and root hair density of tomatoes; using a polarization-hyperspectral imaging system to obtain macroscopic morphological features such as crown width, plant height, and leaf inclination of water stress plants, as well as leaf vein distribution, average gray, and leaf margin shaded area under a water-sensitive wavelength of 1450 nm, and macroscopic morphological features such as polarization states, stock vectors, and Mueller matrix variables of 1450 nm feature images at 0, 45, 90, 135, and 180 feature polarization angles. By fusion of internal and external structures, above-ground, underground, and macroscopic and microscopic morphological features of water stress tomatoes, and mutual fusion of water stress feature wavelength images and polarization state features, advantages are complementary, comprehensive and precise extraction and precise quantitative analysis of water stress features of the tomatoes are implemented, and a basis for scientific management of water and fertilizer integration of facilities is provided.

The invention provides a method of preparing a biological tissue sample and a method of observing a biological tissue section sample that enable stereoscopic observation of a biological tissue easily and rapidly without destroying a biological tissue piece. The method of observing a biological tissue sample according to the invention is a method in which stereoscopic morphology of a biological tissue sample is observed, and the method includes: cutting out a sample having a thickness of 15 to 50 m from a sample block obtained by fixing, dehydrating, and paraffin-embedding a sample cut out from a biological tissue; transferring the sample to a surface-treated slide glass; stretching the sample on the slide glass; performing deparaffinization processing; then, staining the sample with a heavy metal-based staining agent; and observing the stained sample with a scanning electron microscope.

Methods for characterizing living plants, wherein one or more beams of penetrating radiation such as x-rays are scanned across the plant under field conditions. Compton scatter is detected from the living plant and processed to derive characteristics of the living plant such as water content, root structure, branch structure, xylem size, fruit size, fruit shape, fruit aggregate volume, cluster size and shape, fruit maturity and an image of a part of the plant. Ground water content is measured using the same technique. Compton backscatter is used to guide a robotic gripper to grasp a portion of the plant such as for harvesting a fruit.

An analytic method for improving the efficiency in identifying protein molecular effect information using low resolution x-ray crystallography, by selecting and imaging a protein sample with low resolution x-ray crystallography and assaying the data thus generated as to local ligand strain energy value, followed by calculating a real-space difference density Z for each element and compiling ZDD data therefrom, followed by determining the true protomer/tautomer state of the protein sample by calculating Score.sub.i according to the following equation so that the highest Score.sub.i signifies the molecular effect information:

Score.sub.i={((ZDD.sub.i.sub.ZDD)/.sub.ZDD)+((SE.sub.i.sub.SE)/.sub.SE)}.

The present disclosure relates to the field of a cabinet x-ray incorporating an x-ray tube, an x-ray detector, and a real-time camera, either high definition or standard resolution, for the production of organic and non-organic images. The computing device can receive video data from the camera and the x-ray detector and determines, based on the video data, an overlay of the captured x-ray image with the captured real-time image or display images adjacently i.e. Picture-In-Picture (PIP). In particular, the disclosure relates to a system and method with corresponding apparatus for capturing a real-time image simultaneously with the x-ray image allowing a cabinet x-ray unit to attain and optimize images with exact orientation of the 2 images.

An information processing apparatus comprises: an obtainment unit configured to obtain a radiation image of a subject; a generation unit configured to generate, based on a plurality of radiation images obtained by radiation of mutually different spectra, a material characteristic image in which a region for each material can be extracted from the interior of the subject; and an image processing unit configured to perform processing of enhancing or attenuating, based on a position of a specific region in the material characteristic image, the specific region in the radiation image.