The present invention features biomarkers capable of diagnosing inflammatory bowel disease and methods of using such biomarkers to diagnose and selecting treatments for inflammatory bowel diseases.

Assays used in conjunction with a thermal contrast reader are disclosed. In the assay, the test strip includes materials that can develop a thermal response if a target analyte is present in a sample. Linear flow assays include nanoparticles with high affinity binding to the analyte. Binding of the nanoparticles with an analyte in the sample is detected using thermal contrast. Analytes over a broad range of concentrations are detected in the linear flow assays. Methods of detecting target analytes and kits comprising lateral flow assays and thermal contrast reader are also disclosed.

The present invention discloses a preparation method of Cryptococcus neoformans capsular polysaccharide GXM as well as a GXM antigen immunoassay kit and an application thereof. The preparation method of the Cryptococcus neoformans capsular polysaccharide GXM effectively avoids the use of a toxic chemical reagent, ensures safety of operators, and also avoids environmental pollution, has high specificity, and can prepare a high-purity Cryptococcus neoformans capsular polysaccharide while simplifying a preparation process. The GXM antigen immunoassay kit adopts a competition method, has good sensitivity, specificity, repeatability and stability, has high recovery rate of a target compound and may provide more accurate and reliable inspection results. The kit is simple and feasible in use and operation, rapid and sensitive in detection and low in price and provides an effective tool for clinical detection of GXM.

Compositions and methods for displaying antibodies in the periplasmic space of yeast cells are disclosed. In particular, antibodies are linked to a cell membrane-spanning transmembrane domain, a cell-membrane associated protein domain that is on the external face of the yeast cell membrane, a protein that binds to the inner face of the yeast cell wall, or a periplasmic protein in order to display the antibodies in the yeast periplasmic space. In addition, a target protein of interest can be coexpressed in yeast such that it is localized to the plasma membrane or periplasmic space and accessible to binding by displayed antibodies. The disclosure further relates to high-throughput screening of antibody libraries using yeast cell periplasmic display.

The method for particle analysis includes a first magnetic susceptibility measurement step S4 of measuring a volume magnetic susceptibility of each of first particles p1; an encapsulation treatment step S5 of performing an encapsulation treatment so that the first particles p1 encapsulate an encapsulation target component pt smaller than the first particles p1; a second magnetic susceptibility measurement step S8 of measuring a volume magnetic susceptibility of each of second particles p2 as an analysis target that are the first particles p1 after the encapsulation treatment; and a step S9 of analyzing whether or not the encapsulation target component pt is encapsulated in the second particles p2 based on a result of measurement in the first magnetic susceptibility measurement step S4 and a result of measurement in the second magnetic susceptibility measurement step S8.

The present invention relates to methods of identifying substances that modulate GA action through targeting its receptor or acting as a GA functional analog, sensor peptides especially designed for that methods as well as a strain of the species Saccharomyces cerevisiae expressing such a sensor peptide.

A radiation sensor, comprising a housing, a first chamber disposed in the housing and configured to contain a microorganism. A second chamber is disposed in the housing and configured to contain a fermentation material, the second chamber separated from the first chamber by a breakable separator. A breaking member is configured to break the breakable separator when pressed by a user. A flexible membrane is configured to flex when the microorganism ferments and thereby releases a gaseous byproduct. An electronic indicator is configured to relay information indicating the amount of fermentation, when the radiation sensor has been exposed to radiation less fermentation takes place resulting in a smaller volume of released gaseous byproduct.

The subject invention provides methods and means to detect incidents of accidental or intentional release of chemical and biological toxins into the environment by measuring cellular stress-induced proteins in eukaryotic cells exposed to environmental samples suspected of containing chemical or biological toxins using a highly sensitive on-chip surface-enhanced Raman spectroscopy (SERS)-linked immunosensors assay allowing robust, fast, and reliable in-the-field global sensing of environmental threats in resource-limited settings.

A method for analyzing a liquid sample, such as wine, in order to detect the possible presence of sulphite-resistant yeasts of the Brettanomyces bruxellensis species. The method includes detecting whether yeasts of the Brettanomyces bruxellensis species belonging to the genetic group of triploid oenological yeasts are found in the sample. If such yeasts are detected, then the sample is deduced to contain sulphite-resistant yeasts of the Brettanomyces bruxellensis species.

Methods, kits, and diagnostic devices are disclosed for diagnosing an invasive fungal infection in a subject by measuring a T-cell interferon gamma (IFN-) response after exposure to a fungal antigen.