Provided herein are methods and kits for analyzing a biological sample obtained from a subject having, suspected of having, or being at risk for a disease associated with the contact activation system.

Methods for identifying patients with anemia, distinguishing thalassemia-trait anemia from iron-deficiency anemia, and identifying pre-anemic patients several weeks before anemia becomes clinically detectable. Also, methods for detecting blood doping in athletes and for optimizing therapy with erythropoiesis stimulating agents or iron supplementation. Computer-readable storage devices and systems, e.g., for use in the described methods.

The invention provides methods of simultaneously detecting one or more biomarkers associated with one or more platelets in a platelet sample by contacting the sample with one or more metal-tagged probes or mixtures thereof; washing the sample to remove unbound probes; and analyzing the sample by mass cytometry to simultaneously detect binding of the one or more metal-tagged probes or mixtures thereof to one or more biomarkers associated with the one or more platelets. Compositions, panels and kits for use with the methods described herein are also provided.

The present disclosure provides a dry reagent and a method that enable fibrinogen determination without dilution of the sample. More specifically, the present disclosure provides a fibrinogen measurement dry reagent of an undiluted sample comprising: (i) thrombin or a protein having thrombin activity; (ii) magnetic particles; (iii) a fibrin monomer polymerization inhibitor; (iv) a calcium salt; (v) a dry reagent layer solubility improving agent; (vi) a dry reagent layer reinforcing material; and (vii) a buffer and a method for fibrinogen determination.

A composition for blood coagulation testing and the use thereof, and more particularly a composition for blood coagulation testing containing a hemostatic enzyme and carboxymethyl chitosan and the use thereof is disclosed. The composition for blood coagulation testing is useful because it may perform a blood coagulation test with high sensitivity and high speed by improving the activity of the hemostatic enzyme and clot strength and increasing the rate of fibrin formation.

The present invention is directed to a cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid, in particular a blood sample, comprising a cartridge body having at least one measurement cavity formed therein and having at least one probe element arranged in said at least one measurement cavity for performing a test on said sample liquid; and a cover being attachable on said cartridge body; wherein said cover covers at least partially said at least one measurement cavity and forms a retaining element for retaining said probe element in a predetermined position within said at least one measurement cavity. The invention is directed to a measurement system and a method for measuring viscoelastic characteristics of a sample liquid.

Compositions and methods useful for point of care testing for hemophilia by measuring coagulation factor levels are provided.

An exemplary portable automated device is developed for in vitro testing of blood vascular occlusion as a result of sickle cell disease. The portable automated device may be controlled by a computer (e.g., smartphone) application. Calibration of the portable device may be performed using a component of known impedance value. With the developed portable automated device, analysis may be performed on sickle cell samples on a microfluidic platform that mimics the structure of human capillaries. Significant differences in cell impedance signals may be observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.

The present invention is directed to a cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid, in particular a blood sample, comprising a cartridge body having at least one measurement cavity formed therein and having at least one probe element arranged in said at least one measurement cavity for performing a test on said sample liquid; and a cover being attachable on said cartridge body; wherein said cover covers at least partially said at least one measurement cavity and forms a retaining element for retaining said probe element in a predetermined position within said at least one measurement cavity. The invention is directed to a measurement system and a method for measuring viscoelastic characteristics of a sample liquid.

A handheld medical analyzer works with different disposable application cartridges to perform a variety of interrogations on specimen samples. One application includes attaching a biological microelectromechanical systems (BioMEMS) cartridge that generates blood coagulation profiles indicative of particular forms of coagulation disorders. The device makes coagulopathy testing simpler for small hospitals, clinics, ambulances, remote locations and individuals and permits for a larger number of parallel or serial devices operating simultaneously. One insertion of a cartridge actuates an oscillating circular motion to generate a blood coagulation profile based on a change in rotational motion as blood coagulates in a sample. Change in rotational motion is analyzed through a video camera such as in a smartphone and is plotted to show an amplitude over time. Actuation of the BioMEMS can be achieved by magnetic actuation of a motor controlled by an iPhone or a smart phone to provide a specific rotational pattern.