A computer-based method for determining a prediction of risk and/or an indication of extent of coronary stenosis in a human subject, comprises the steps of: (a) inputting the level of at least one cholesteryl ester measured in a blood sample collected from said subject; and then (b) inputting the age and gender of said subject; and then (c) generating in said computer from said cholesteryl ester level input, said age input and said gender input a prediction of risk and/or an indication of extent of coronary stenosis in said subject. Systems and methods are also described.

The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides the necessary information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired.

Compositions and methods are provided for diagnosis and/or prognosis of peripheral artery disease, aortic stenosis, or cardiovascular events in a subject. In some embodiments, the method includes measuring and comparing the level of particular proteins to other proteins. In other embodiments, the method includes comparison with clinical variable information.

A method of diagnosing peripheral artery disease in a mammalian subject is provided, including a method of distinguishing advanced disease from a less advanced form, e.g. CLTI from IC, based on the level of one or more metabolic biomarkers selected from: creatine, creatinine, carnitine, propionylcarnitine, cystine, lysine, tyrosine, histidine, phenylacetylglutamine, oxoproline, arginine, monomethylarginine, a fatty acid biomarker selected from the group consisting of stearic acid, linoleic acid, heptadecanoic acid, palmitic acid, oleic acid, heptadecenoic acid, pentadecanoic acid and eicosadienoic, and a ratiometric biomarker comprising at least one of the metabolic biomarkers.

The object of the present invention is to provide an easy and quick detection method for detecting an oxLDL/β.sub.2GPI complex in biological samples, and a detection kit therefor. The present invention attains this object by providing a detection method for detecting an oxLDL/β.sub.2GPI complex, which uses a test strip for lateral flow assay, comprising a step of capturing the oxLDL/β.sub.2GPI complex in the test sample in a predetermined position on the test strip by a first binding component that binds to the oxLDL/β.sub.2GPI complex; and a step of labeling the oxLDL/β.sub.2GPI complex captured in the predetermined position on the test strip by making a second binding component comprising a labeling agent be bound to the captured oxLDL/β.sub.2GPI complex, and a detection kit therefor.

The present invention is intended to provide a novel biomarker for detecting arteriosclerosis-related disease or for evaluating the stage of progression of arteriosclerosis, and specifically, the present invention relates to a marker for detecting arteriosclerosis-related disease or for evaluating the stage of progression of arteriosclerosis, comprising an NPC2 (Niemann-Pick disease type C2) protein and/or an IGFBP7 (Insulin-like growth factor-binding protein 7) protein.

The present invention relates to PIGR, APOA, HPT, HEP2, C5, ITIH1 and IGHA2 as biomarkers for the screening, diagnosis and/or monitoring of subclinical atherosclerosis and methods and kits using thereof.

A method for processing angiography image data by using an imaging catheter path that is directly detected from the angiography data as a co-registration path or using detected marker locations from the angiography data to generate a co-registration path. If the acquired angiography data includes synchronized cardiac phase signals and a predetermined quantity of angiography image frames not including contrast media, then a directly detected imaging catheter path is used as the co-registration path. Otherwise the co-registration path is determined based upon detected marker locations from the angiography image data.

Provided are a method for acquiring a value relating to a triglyceride metabolic capacity of a subject within a shorter period of time compared to conventional nuclear medicine methods, and a test reagent and a test kit that are to be used in the acquisition method. This problem can be solved by an acquisition method of a value relating to the triglyceride metabolic capacity in leucocytes of a subject, said method comprising: a first step of mixing, in vitro, a fatty acid compound labeled with a fluorescent substance with leucocytes collected from the subject and thus bringing the fatty acid labeled with the fluorescent substance into contact with the leucocytes, wherein the fatty acid compound contains a fatty acid residue, the fatty acid residue has 8-26 carbon atoms, and a part of hydrogen atoms constituting the fatty acid residue, excluding a terminal methyl group of the fatty acid residue, may be substituted by an alkyl group having 1-3 carbon atoms; and a second step of acquiring, as the value relating to the triglyceride metabolic capacity, a measured fluorescence intensity that is derived from the fluorescent label in the leucocytes.

The present invention pertains to a silkworm-type biotinylated CTLD14 or sRAGE and a method for manufacturing the same. One embodiment of the present invention provides a method for manufacturing biotinylated proteins, wherein the method includes A) a step for inserting a nucleic acid molecule for coding biotin ligase and protein in a coexpressable manner into a silkworm or a living organism that imparts sugar chains that are the same as the sugar chains of the silkworm, B) a step for causing the biotin ligase and protein to be expressed by disposing the silkworm or the living organism that imparts sugar chains that are the same as the sugar chains of the silkworm to conditions with which the nucleic acid molecule will carry out expression, and C) a step for administering biotin to the living organism and obtaining the biotinylated protein.