The present invention relates to A device for performing an assay on an aqueous biological sample, the device comprising: (i) at least a first chamber comprising a desiccated hydrogel, wherein the hydrogel incorporates at least a first desiccated assay reagent or reagents, the hydrogel and reagent or reagents being configured to allow the controlled release of the first reagent or reagents when the hydrogel has been exposed to the aqueous biological sample and has at least been partially hydrated; and (ii) an imaging device for imaging and analysing the first chamber after the aqueous biological sample has been introduced into the first chamber and the hydrogel has been at least partially hydrated and at least part of the first reagent or reagents has or have been released. The present invention also relates to a device for rapidly determining the susceptibility of a microorganism in an aqueous biological sample to an antimicrobial agent comprising: a) a multi-chamber plate, wherein: (i) at least a first chamber comprises a growth medium, a first dye, and a desiccated hydrogel; and (ii) at least a second chamber comprises a growth medium, a first dye, a desiccated hydrogel, and a first antimicrobial agent that inhibits or slows the proliferation of the, or a, microorganism; and b) an imaging device for imaging and analysing one or more chambers after the biological sample has been introduced into the chamber and the hydrogel has been at least partially hydrated. The invention also relates to related methods and a kit of parts. The device is particularly suited for identifying the which antimicrobial agents would be suitable for the treatment of microbial infections, such as Urinary Tract Infections (UTIs).

Methods and systems for identifying and/or treating urinary disorders are provided. The methods and systems generally operate by obtaining a urine sample from a subject, identifying (such as by using nucleic acid sequencing) an abundance of a first set of one or more microbes (such as one or more bacteria or viruses) in the urine sample, and determining whether the subject suffers from a urinary disorder based on the abundance of the first set of one or more microbes. In some cases, the methods and systems further operate by identifying a second set of microbes to supplement a microbiome in the urinary tract of the subject. In some instances, the methods and systems further operate by treating the urinary disorder using the second set of microbes. In some instances a preservation solution is utilized.

The disclosure provides compositions and methods for the detection of renal disease and periodontal disease in mammals.

Methods and systems for identifying and/or treating urinary disorders are provided. The methods and systems generally operate by obtaining a urine sample from a subject, identifying (such as by using nucleic acid sequencing) an abundance of a first set of one or more microbes (such as one or more bacteria or viruses) in the urine sample, and determining whether the subject suffers from a urinary disorder based on the abundance of the first set of one or more microbes. In some cases, the methods and systems further operate by identifying a second set of microbes to supplement a microbiome in the urinary tract of the subject. In some instances, the methods and systems further operate by treating the urinary disorder using the second set of microbes. In some instances, a preservation solution is utilized.

The disclosure provides compositions and methods for the detection of renal disease and periodontal disease in mammals.

A diagnostic kit and method for detecting urinary tract infections (UTIs) and bacteremia using a drinking water test in low-resource settings is provided. The kit includes a first bag containing a first culture medium capable of detecting Gram-negative and coliform bacteria through colorimetric and fluorescence indicators, and a second bag containing a second culture medium capable of detecting Gram-positive bacteria through colorimetric indicators. A method of using the kit involves inoculating a sample into each bag, incubating the bags, and observing for color changes and fluorescence to indicate bacterial presence. The kit enables rapid, cost-effective detection of bacterial pathogens in urine and blood samples without specialized laboratory equipment, facilitating early diagnosis and treatment in resource-limited environments.