Device and system for characterizing samples using multiple integrated tips scanning probe microscopy. Multiple Integrated Tips (MiT) probes are comprised of two or more monolithically integrated and movable AFM tips positioned to within nm of each other, enabling unprecedented micro to nanoscale probing functionality in vacuum or ambient conditions. The tip structure is combined with capacitive comb structures offering laserless high-resolution electric-in electric-out actuation and sensing capability and novel integration with a Junction Field Effect Transistor for signal amplification and low-noise operation. This platform-on-a-chip approach is a paradigm shift relative to current technology based on single tips functionalized using stacks of supporting gear: lasers, nano-positioners and electronics.

System and method for measuring an optical property of a sub micrometer region of a sample including interacting a probe tip of a probe microscope with a region of the sample, illuminating the sample with a beam of light from a radiation source such that light is scattered from the probe-sample interaction region, interfering a reference beam with the scattered light wherein the reference beam has an adjustable optical phase, measuring with a detector at least a portion of the light scattered from probe-sample and background regions at a substantially constant reference phase, and constructing a signal indicative of the optical property of the sample wherein contributions from background scattered light are substantially suppressed.

A scanning probe microscope, in particular an atomic force microscope, for analyzing a sample by moving a probe and the sample relative to one another, wherein the scanning probe microscope includes a detection unit with a side view camera arranged and configured for detecting an image of the sample in a substantially horizontal side view, and a determining unit for determining information indicative of a profile of at least part of a surface of the sample based on the detected image.

The present invention relates to an apparatus and method for generating a three-dimensional image of a polymer substance. The three-dimensional image generating apparatus of the present invention comprises: a specimen state adjustor for adjusting a temperature or pressure of a solid specimen in order to maintain, in a solid state, the solid specimen including a plurality of polymer substances; an image collector for collecting a partial image of the plurality of polymer substances exposed on a surface of the solid specimen; a low molecule image database for storing an image of an element low molecule substance; and an image processor for generating a three-dimensional image of the polymer substance by matching the collected partial image with an image in the low molecule image database.

An observation method using a compound microscope of an inverted optical microscope and an atomic force microscope includes scanning a cantilever so that a probe approaches a sample until surface layer information is acquired, observing the cantilever through the optical microscope to acquire shape information of the cantilever, moving an observation position of the optical microscope downward based on a length of the probe, performing fluorescence observation through the optical microscope, and scanning the cantilever to acquire the surface layer information. The probe approach, cantilever observation, and observation position movement are performed in order. The fluorescence observation is performed after the observation position movement. The surface layer information acquisition is performed after the probe approach.

A sample vessel retention mechanism for an inverted microscope having an optical objective and a scanning probe microscope (SPM) head. The inverted microscope includes a platform for supporting a sample vessel, in which is formed an aperture sized to provide a passage for the objective of the inverted microscope to approach the sample vessel from below. The retention mechanism provides a vacuum region formed in the platform, with the vacuum region being barometrically coupled with a vacuum generator. Establishment of a vacuum in the vacuum region prevents or substantially reduces oscillation of the sample vessel floor in an operating frequency range of the SPM head.

The invention is directed at a method of refurbishing a probe for use in a scanning probe microscopy device, wherein the probe is a used or damaged probe and includes a cantilever and a probe tip. The method comprises receiving the probe, determining an existing probe structure of the probe and mapping the existing probe structure for obtaining existing probe structure data. The method further includes identifying, based on the existing probe structure data, a deviation from an original probe structure of the probe prior to said using or damaging thereof. Based on the deviation, structural modification data indicative of a structural modification for modifying the probe will be determined, and, in accordance with the structural modification data, the existing probe structure will be modified by at least one of a precision material deposition process or precision material removal process, for performing said refurbishing of the probe. The method is further directed at a system and a computer program product for operating a system.

An atomic force microscope is provided having a controller configured to store one or more positional parameters output by a sensor assembly when a light spot is located at a first preset position on the surface of the cantilever. The controller is further configured to operate an actuator assembly so as to induce movement of the spot away from the first preset position, to detect said movement of the first spot based on a change in the one or more positional parameters output by the sensor assembly, and to operate an optical assembly in response to the detected movement of the first spot to return the first spot to the first preset position.

The present disclosure provides a system and method for a fiber-coupled, metal-tip chemical imaging spectroscopy. The system couples the electromagnetic radiation (EMR), such as laser light, through an optical fiber to a conductive tip for both EMR excitation to the sample through the conductive tip and EMR signal collection from the sample through the conductive tip. The system and method effectively eliminates the need for an optical alignment between the EMR source and the tip, and still offers the customary spatial resolution of a non-coupled system.

Provided is a scanning probe microscope capable of performing observation with high accuracy even when a beam splitter is configured to be movable. When checking positions of a sample and a cantilever in a scanning probe microscope, by disposing an optical microscope to face a first opening portion of a top surface of a housing, and by gripping and rotating an operating portion provided on a side surface of the housing, a user rotates and moves a beam splitter held by a holding portion in the housing, and retracts the beam splitter from the field of view of the optical microscope. Therefore, the beam splitter can always be disposed in the housing, and the user can be prevented from touching the beam splitter. As a result, it is possible to prevent the beam splitter from being damaged or stains from adhering to the beam splitter. Further, the moving distance of the bears splitter 6 can be shortened. Therefore, it is possible to suppress the occurrence of a deviation in the position of the beam splitter.