Real-time focusing in a slide-scanning system. In an embodiment, focus points are added to an initialized focus map while acquiring a plurality of image stripes of a sample on a glass slide. For each image stripe, a plurality of frames, collectively representing the image stripe, may be acquired using both an imaging line-scan camera and a tilted focusing line-scan camera. Focus points, representing positions of best focus for trusted frames, are added to the focus map. Outlying focus points are removed from the focus map. In some cases, one or more image stripes may be reacquired. Finally, the image stripes are assembled into a composite image of the sample.

A surgical microscope, a method for observing an object in an observation area during surgery, and a retrofit-kit for a surgical microscope are provided. The surgical microscope includes at least one optical carrier for variably deflecting an observation axis of an optical observation assembly into an optical viewing axis directed towards the observation area. The optical carrier includes at least one optical beam deflector and is arranged between the optical observation assembly and the observation area. The optical carrier further includes a movable range-setting system for supporting the at least one optical beam deflector and for positioning the at least one optical beam deflector at a variable distance from the optical observation assembly.

A method for imaging a sample using a microscope includes recording a first image of the sample, the first image being represented by image data. Sample information is extracted from the first image by analyzing the image data using an analyzer. At least a part of the sample is scanned with a light beam while modulating the light beam based on the extracted sample information. A second image of the sample is recorded during and/or after scanning the sample with the modulated light beam.

An in vivo of insitu bio-matter sample magnified digital image creating device for realtime biopsy determinations having a sharp edged window pocket coupled to housing having an optical and digital magnification path coupled to image detecting sensor logic. The device has an optical two blade window pocket rotatably coupled to a stem mechanically controlled and extendable from mechanism within the housing, the stem having source for at least one fiber optical channel for selected frequency and wavelength light various light sources, an optical two blade window pocket slide component optically coupled to the two blade window pocket fiber optic channel stem distal end, providing light through the two blade window pocket slide normal axis surface for illumination penetrating a bio matter assay or sample for imaging through an optical microscopy magnification path axis to a micrograph image sensor. The resulting image micrographs for digital pathology realtime result determination.

The multi-photon microscope comprises a repetition rate tuner that lowers an optical pulse train emitted from a pulsed laser to a repetition rate for time-gated detection, a scanner that scans the optical pulse train transmitted from the repetition rate tuner in x-axis and y-axis directions, an objective lens that irradiates an optical signal scanned by the scanner to the sample and acquires a fluorescence signal emitted from the excited fluorescent material, a photodetector that photoelectrically converts the fluorescence signal acquired by the objective lens, an amplifier that converts a current signal output from the photodetector into a voltage signal, a digitizer that samples the voltage signal output from the amplifier, and a computing device that separates sampling data output from the digitizer with a detection window set in time domain, and generates an image based on the sampling data separated by the detection window.

Systems and methods for managing a plurality of scanning devices in a high-throughput laboratory environment. Each of the scanning devices is configured for a remote boot operation from an administrative server that is communicatively coupled with the plurality of scanning devices via a local network. The remote boot replaces the complete operational firmware of a scanning device. The scanning devices are each configured to periodically provide operational information to the administrative server for centralized storage. The centralized storage of operational information for each of the plurality of scanning devices, coupled with the ability of the administrative server to initiate a reboot of any scanning device and thereby update the complete operational firmware of the scanning device, allows for centralized administration of multiple scanning devices that facilitates configuration, support, image data storage, and/or communication with outside servers.

The disclosure provides a system that may: provide multiple first portions of a laser beam to an objective lens of an optical system; provide the multiple first portions of the laser beam to respective multiple locations of a test surface; receive multiple second portions of the laser beam from the test surface; determine multiple intensities respectively associated with the multiple second portions of the laser beam; transform the multiple intensities into data that represents multiple measurement values of the multiple intensities; determine, from the data, if an intensity value of the multiple intensities is below a threshold intensity value; if the intensity is below the threshold intensity value, provide information that indicates an issue associated with the objective lens; and if the intensity is not below the threshold intensity value, provide information that indicates there is no issue associated with the objective lens.

Provided are a pulse compressor and a two-photon excited fluorescence microscope. The microscope includes a light source which generates a laser beam having a pulse, a pulse compressor which compresses the pulse of the laser beam, an objective lens which provides the laser beam to a specimen, and image sensors which receive the laser beam and obtain images of the specimen. The pulse compressor may include a grating plate, a corner cube provided on one side of the grating plate, and a retroreflector provided on the other side of the grating plate.

A method for obtaining image data of a subject, including; a first scanning step including a plurality of steps W, each step W being a step of determining one place existing in a one-dimensional, two-dimensional, or three-dimensional first space; and a second scanning step of scanning insides of second spaces including at least one of the places, wherein the second scanning step includes a step X of randomly determining a location of an observation point and a step Y of obtaining a piece of image data for the observation point, and, at a time point of end of scanning an inside of one of the second spaces, the second space has a first region including 50% of observation points and a second region existing outside the first region and including remaining 50% of the observation points, the second region being larger than the first region by at least 15%.

A method for timing procedures in a microscope system, which has a plurality of microscope modules configured to carry out various processes, provision is made for a clock signal to be provided to all microscope modules by a central clock generator and for the clock signal to be modulated by a clock modulation circuit in order to produce a defined clock-pulse number. The microscope modules define a start time for carrying out a process by way of the clock-pulse number, carrying out the process as soon as the clock-pulse number is reached. Moreover, a corresponding microscope system is described.