A microscope and method of microscopy having a light source for providing illumination light, a controllable manipulation device for generating in a variable manner an illumination pattern of the illumination light to be selected, an illumination beam path with a microscope lens for guiding the illumination pattern to a sample to be examined, a detector having a plurality of pixels for examining the fluorescent light emitted by the sample, a detection beam path for guiding the fluorescent light emitted by the sample to the detector, a main beam splitter for splitting illumination light and fluorescent light, a control and evaluation unit for controlling the manipulation device and for evaluating the data measured by the detector. The manipulation device is arranged in the illumination beam path upstream from the main beam splitter in the vicinity of an optically conjugated plane to the sample plane such that the pixel of the detector can be individually activated using the control and evaluation unit and in read out patterns to be selected and that the control and evaluation unit is designed to activate pixels of the detectors individually or in a selected read out pattern dependent on the selected illumination pattern.

A kit (10) includes a light source (12) and an optical system (14) equipped with a lens assembly (25) defining a magnification optical axis (X-X). A frame (16) is crossable by the light generated by the light source (12). The frame (16) is configured for supporting a sample holder (H), a portable electronic apparatus (S) equipped with an image acquisition device (C), and the optical system (14), which are interposable between the sample holder (H) and the image acquisition device (C). The optical system (14) is configured for being movable in a guided manner on the frame (16), to allow aligning the optical axis (X-X) with the image acquisition device (C). A carrying body (18) is configured for receiving in abutment the frame (16) and housing the light source (12) directing light towards the optical system (14) through the frame (16).

Provided herein are systems and methods for simultaneous imaging and stimulation using a microscope system. The microscope system can have a relatively small size compared to an average microscope system. The microscope can comprise in part an imaging light source and a stimulation light source. Light from the imaging light source and the stimulation light source can be spectrally separated to reduce cross talk between the stimulation light and the imaging light.

This disclosure discloses a microscope system, a smart medical device, an automatic focusing method, and a storage medium. The smart medical device includes an objective lens, a beam splitter, an image projector assembly, a camera assembly, and a focusing device. The objective lens includes a first end and a second end, and the first end faces a to-be-observed sample. The beam splitter is disposed on the second end. The image projector assembly is in communication with the beam splitter, the image projector assembly includes a first lens and an image projection device, and light generated by the image projector assembly enters the beam splitter through the first lens. The camera assembly includes a camera. The focusing device is disposed on the camera assembly, and the focusing device is configured to perform focus adjustment on the camera.

A device for machining an object by laser radiation, by photodisruption. The device includes an observation device for imaging the object and a laser scanning device by which the laser radiation is passed over a predetermined sector of the object for scanning the sector. The device includes the observation device with a first lens for imaging the object; the laser scanning device with a second lens, through which the laser radiation is guided, in which both lenses with regard to the dimension of the regions to be produced in the images and/or with regard to their focal intercept are different from each other. The device alternately images the respective region of the object in a first operating mode by the first lens and in a second operating mode by the second lens. It is thus possible to use in both operating modes a lens adapted to the intended imaging purpose.

The present invention discloses a portable zoom microscope, comprising a case (15) and an optical system assembly installed in the case (15), characterized in that the optical system assembly comprises an objective I (1), an objective II (2), an eyepiece III (3), an eyepiece II (4) and an eyepiece I (5) arranged in sequence along an optical axis Z from an object plane to eyes, wherein the objective I (1) is a positive lens, the objective II (2) is a negative lens, the eyepiece III (3) is a positive lens, the eyepiece II (4) is a positive lens, and the eyepiece I (5) is a negative lens, which effectively eliminates the longitudinal chromatic aberration and the lateral colour, so that the zoom microscope can distinguish the details of small objects more clearly and intuitively, greatly improving the image quality of products.

A digital microscope system comprises an imaging device configured to generate digital image data representing a target region of an object, the target region being determined by a changeable setting of the imaging device; and a controller configured to generate monitor image data corresponding to the digital image data generated in accordance with the setting, the monitor image data being configured to be displayed as a monitor image; wherein the controller is further configured to change the setting in response to a user input; and wherein the controller is further configured to compensate for a delay in updating the monitor image data in accordance with the changed setting by storing the digital image data generated in accordance with the unchanged setting in response to the user input and generating simulation monitor image data by performing digital image processing on the stored digital image data taking into account the changed setting, the simulation monitor image data being configured to be displayed as a simulation monitor image during the delay.

A metrology system is provided including a projected pattern for points-from-focus type processes. The metrology system includes an objective lens portion, a light source, a pattern projection portion and a camera. Different lenses (e.g., objective lenses) having different magnifications and cutoff frequencies may be utilized in the system. The pattern projection portion includes a pattern component with a pattern. At least a majority of the area of the pattern includes pattern portions that are not recurring at regular intervals across the pattern (e.g., as corresponding to a diverse spectrum of spatial frequencies that result in a relatively flat power spectrum over a desired range and with which different lenses with different cutoff frequencies may be utilized). The pattern is projected on a workpiece surface (e.g., for producing contrast) and an image stack is acquired, from which focus curve data is determined that indicates 3 dimensional positions of workpiece surface points.

Certain disclosed embodiments concern an integrated imaging system that combined light-sheet microscopy, which enables considerable speed and phototoxicity gains, with quantitative-phase imaging. A method for using such imaging systems also is disclosed. In an exemplary embodiment, an integrated imaging system was used for multivariate investigation of live-cells in microfluidics.

A lens attachment module is configured for coupling with a zoom module of a finite conjugate optical assembly. The lens attachment module includes a lens assembly that has a positive focal length, and exhibits a pupil size of between 16 and 25 mm and a pupil depth greater than 50 mm.