In accordance with one embodiment of the present invention an apparatus for a low numerical aperture exclusion imaging apparatus is provided. The apparatus may include an electromagnetic illumination source for illuminating a portion of a specimen; and for collecting an image created by the electromagnetic radiation an objective lens optically coupled to the electromagnetic illuminated portion of the specimen. The apparatus also includes an optical blocking plate disposed between the objective lens and a focusing lens. The optical blocking plate is positioned to substantially block undesired electromagnetic radiation from image sources distally aligned in the same optical axis as the specimen. This invention is enhances narrow depth of field characteristics in imaging. It also enhances discreet imaging in a narrow focus field by eliminating some or most of the light which contributes to wide depth of field focus. This is useful for optical sectioning ranging from microscopy to photography. Optical sectioning provides the information necessary for 3D image reconstructions and other X Axis spatial measurements.

The invention relates to a microscope (10) having a movable stage (18) and an adjusting unit (24) for moving the stage (18). The adjusting unit (24) comprises a drive shaft (38) by the rotation of which the distance between the stage (18) and a base (22) of the microscope (10) is adjustable. Further, the microscope (10) has a manually operable operating unit (26) with a first output shaft (46) as well as an electric drive unit (28) for the motorized movement of the stage (18) with a second output shaft (52). In a manual operating mode, the drive shaft (38) is coupled to the first output shaft (46) via a first coupling unit (54), and in a motorized operating mode the drive shaft (38) is coupled to the second output shaft (52) via a second coupling unit (56).

The invention relates to a microscope (10) that encompasses an objective system (30) and a zoom system (32). The microscope furthermore has a diaphragm (60) for limiting the aperture of the beam path. A control unit (64) is furthermore provided, that control unit (64) automatically ascertaining, as a function of the current manifestation of at least one parameter of the microscope (10), a respective setting of the diaphragm (60) predetermined for the current manifestation, and setting the diaphragm (60) accordingly.

A device may use a camera to capture an image of an end face of an optical fiber in a field of view of the camera. The device may monitor a focus metric associated with the image while the image is manually focused using an opto-mechanical assembly. The device may automatically initiate a test to inspect the image of the end face of the optical fiber for compliance with a set of criteria related to cleanliness and damage based on the focus metric satisfying a condition. The device may output a result from the test indicating whether the end face of the optical fiber satisfies the set of criteria related to cleanliness and damage.

A digital microscope and a focusing thereof are provided. The microscope integrates a focusing lens unit, a zooming lens unit and related control circuits into the interior of the microscope. The control unit executes a focus searching algorithm and focus tracking algorithm, controls movement of the focusing lens unit and the zooming lens unit and achieves auto-focus by adjusting the image distance and makes the microscope zoom by adjusting the focal length. This improves the focusing efficiency and shortens the focusing time greatly. The microscope can achieve continuous zooming and tracking by the zoom tracking algorithm and the image is kept clear in the zooming process. The microscope adjusts the brightness of the assist light source at the front end of the microscope according to the change of the amount of light transmission by the control unit executing the exposure algorithm to ensure uniform exposure.

A three-dimensional image is easily, quickly, and efficiently acquired, regardless of the shape of a specimen or other factors. The present invention provides a microscope system including: a microscope that acquires an image of a specimen; a reference-shape setting portion that sets an approximate shape of the specimen; an observation-area estimating portion that sets a three-dimensional observation area of the specimen according to the reference shape set by the reference-shape setting portion; and a control unit that controls the microscope so as to acquire a three-dimensional observation image of the observation area set by the observation-area estimating portion.

Humidified sample preparation station for serial crystallography according to one embodiment is a humidified enclosure that delivers relative humidities above 95% and preferably above 97% in standard operation, and that can allow microscope observation of samples within. Humidified sample preparation station for serial crystallography can be used for preparation of protein crystal samples for examination using X-rays and for protein structure determination by X-ray crystallography, involving addition of liquid to the sample and removal of liquid from the sample using vacuum or suction.

A dual-configuration microscope is provided that may be converted into an upright or inverted microscope. The microscope includes a base and a body having a first portion and a second portion, wherein the body is rotatably coupled to the base. The microscope further includes an objective coupled to the first portion of the body, a condenser coupled to the second portion of the body and a stage positioned between the objective and the condenser. The microscope further includes a first and second knob configured to adjust the position of the objective, wherein the first knob is disposed proximal to the first portion of the body and the second knob is disposed proximal to the second portion of the body.

There are provided a microscope apparatus, an observation method, and a microscope apparatus-control program that can more efficiently perform auto-focus control and can shorten an imaging time in a case where a culture vessel is to be scanned by an image forming optical system and the auto-focus control is to be performed at each observation position. Focus information of a culture vessel is detected by a first displacement sensor and a second displacement sensor while a stage is moved to a scanning measurement position from an initial set position, and an auto-focus control unit performs auto-focus control at every observation position on the basis of the focus information in a case where the stage has been moved to the scanning measurement position.

A method and corresponding optical device to correct spherical aberration in an optical path caused by an electrically tunable lens (ETL) within the optical path. The method includes placing within the optical path and in working relationship with the ETL an aspherical correction lens dimensioned and configured to reduce spherical aberration in a light beam exiting the ETL.