Laser 3D imaging techniques include splitting a laser temporally-modulated waveform of bandwidth B and duration D from a laser source into a reference beam and a target beam and directing the target beam onto a target. First data is collected, which indicates amplitude and phase of light relative to the reference beam received at each of a plurality of different times during a duration D at each optical detector of an array of one or more optical detectors perpendicular to the target beam. Steps are repeated for multiple sampling conditions, and the first data for the multiple sampling conditions are synthesized to form one or more synthesized sets. A 3D Fourier transform of each synthesized set forms a digital model of the target for each synthesized set with a down-range resolution based on the bandwidth B.

According to one embodiment, a beam splitter splits light into first light and second light. The second light is used to irradiate a sample containing particles. A first imaging device images a first interference pattern formed by multiplexing third light, which has been generated by irradiating the particles with the second light, and the first light. A second imaging device images a second interference pattern formed by the third light. An arithmetic device compares a composite image with a calculated image. The composite image is created by using a first interference image picked up by the first imaging device and a second interference image picked up by the second imaging device. The calculated image is obtained by combining single particle interference images, each of which is expected to be obtained by the first imaging device in a case where a particle is present alone in the sample.

A digital holographic apparatus includes a first hologram generating unit that generates a first hologram by causing first object light in a first observation direction to interfere with first reference light, the first object light being generated by irradiating an observation object with light having a first wavelength, the first reference light being derived from the light having the first wavelength; a second hologram generating unit that generates a second hologram by causing second object light in a second observation direction that differs from the first observation direction to interfere with second reference light, the second object light being generated by irradiating the observation object with light having a second wavelength, the second reference light being derived from the light having the second wavelength; a first image capturing unit that captures the first hologram; and a second image capturing unit that captures the second hologram.

According to an embodiment, a holographic microscope comprises a light source, an optical system splitting light emitted from the light source into an object and a reflective mirror and inducing interference between light reflected by the object or transmitted through the object and light reflected by the reflective mirror, a first image sensor receiving the interference light and sensing interference information for the interference light, a second image sensor receiving the light reflected by the object or transmitted through the object and sensing information for the received light, and an image processor deriving a shape of the object based on the interference information sensed by the first image sensor and the information sensed by the second image sensor.

An ultra high-resolution near infrared brain imager system includes a modular cap housing closely spaced multiple vertical-cavity surface-emitting laser-single-photon avalanche photodiode array (VCSEL-SPAD) modules, each one of the VCSEL-SPAD modules including a linear VCSEL array and a SPAD detector.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

The present invention can realize both a transmission type and a reflection type, and provides a holographic microscope which can exceed the resolution of the conventional optical microscope, a hologram data acquisition method for a high-resolution image, and a high-resolution hologram image reconstruction method. In-line spherical wave reference light (L) is recorded in a hologram (I.sub.LR) using spherical wave reference light (R), and an object light (O.sup.j) and an illumination light (Q.sup.j) are recorded in a hologram (I.sup.j.sub.OQR) using a spherical wave reference light (R) by illuminating the object with an illumination light (Q.sup.j, j=1, . . . , N) which is changed its incident direction. From those holograms, a hologram (J.sup.j.sub.OQL), from which the component of the reference light (R) is removed, is generated, and from the hologram, a light wave (h.sup.j) is generated. A light wave (c.sup.j) of the illumination light (Q.sup.j) is separated from the light wave (h.sup.j), and using its phase component (.sup.j=c.sup.j/|c.sup.j|), a phase adjustment reconstruction light wave is derived and added up as (H.sub.P=h.sup.j/.sup.j), and an object image (S.sub.P=|H.sub.P|.sup.2) is reconstructed.

A holographic interferometer, comprising: at least one imaging device capturing an interference pattern created by at least two light beams; and at least one aperture located in an optical path of at least one light beam of the at least two light beams; wherein the at least one aperture is located away from an axis of the at least one light beam, thus transmitting a subset of the at least one light beam collected at an angle range.

A hologram image acquiring apparatus may include: a linear polarizer that filters incident light reflected by an object into a polarized component of a specific angle; a spherical lens that partially converts light that is incident through the linear polarizer to a spherical waveform; and a phase shifter that converts a part of the light incident through the spherical lens to a plane waveform having a different phase per pixel unit.

Laser 3D imaging techniques include splitting a laser temporally-modulated waveform of bandwidth B and duration D from a laser source into a reference beam and a target beam and directing the target beam onto a target. First data is collected, which indicates amplitude and phase of light relative to the reference beam received at each of a plurality of different times during a duration D at each optical detector of an array of one or more optical detectors perpendicular to the target beam. Steps are repeated for multiple sampling conditions, and the first data for the multiple sampling conditions are synthesized to form one or more synthesized sets. A 3D Fourier transform of each synthesized set forms a digital model of the target for each synthesized set with a down-range resolution based on the bandwidth B.