An apparatus for inspecting a biological tissue uses a pH-sensitive coating material to determine whether the tissue is normal or cancerous. The coating material is placed in contact with the tissue to be excited by an excitation light. The coating material is arranged to provide a response signal indicative of the pH value of the tissue. Using a fiber bundle having a plurality of optical fibers forming a linear array or a two-dimensional array adjacent the coating material, the imaging of localized surface pH in the biological tissue can be achieved using the response signal through each of the optical fibers. The fiber bundle can be arranged as a probe to examine the tissue for providing direct mapping of the tumor margin via a display, so that a surgeon can inspect the tissue in real-time.

A compact, portable Raman spectrometer makes fast, sensitive standoff measurements at little to no risk of eye injury or igniting the materials being probed. This spectrometer uses differential Raman spectroscopy and ambient light measurements to measure point-and-shoot Raman signatures of dark or highly fluorescent materials at distances of 1 cm to 10 m or more. It scans the Raman pump beam(s) across the sample to reduce the risk of unduly heating or igniting the sample. Beam scanning also transforms the spectrometer into an instrument with a lower effective safety classification, reducing the risk of eye injury. The spectrometer's long standoff range automatic focusing make it easier to identify chemicals through clear and translucent obstacles, such as flow tubes, windows, and containers. And the spectrometer's components are light and small enough to be packaged in a handheld housing or housing suitable for a small robot to carry.

Disclosed herein are systems and methods of obtaining a derivative Raman spectrum using an excitation or Raman pump beam whose wavelength is modulated in any suitable manner such as, for example, stochastically. Shifting the wavelength of the input excitation by a small amount in approaches like SERDS can isolate the Raman scatter from other spectral artifacts and reduce the false detection rate. For example, an input excitation sequence can be correlated with the response of an individual pixel of a detector. From this, pixels that have captured Raman scattered photons can be separated from pixels capturing non-Raman photons. These techniques can be expanded to other fields and/or types of spectroscopies that utilize a dispersive element detector with time-dependent spectral features.

Aspects of Raman spectrum plane imaging device(s), belonging to the technical field of Raman spectra, are disclosed. In one example, a Raman spectrum plane imaging device may comprise a laser generation apparatus capable of adjusting an output wavelength, a light filtering apparatus, and a planar array detector. Laser light beams emitted by such laser generation apparatus may irradiate on a surface of a sample in a planar illuminating manner. According to systems herein, Raman scattered light generated by the sample under the excitation of the laser light beams is incident on the light filtering apparatus and is imaged on the planar array detector after selectively passing through the light filtering apparatus, to be received by the planar array detector. In some implementations, the light filtering apparatus may comprise an F-P interference device and a band-pass light filter.

Disclosed is an apparatus and method for processing a bio optical signal based on a spread spectrum scheme including a demodulator configured to collect a bio optical signal generated in response to an incident beam modulated based on a spreading code being scattered from a target analyte, and remove a noise from the bio optical signal by demodulating the bio optical signal based on the spreading code, wherein the bio optical signal has a correlation with the modulated incident beam.

A Raman spectroscopy device includes: an irradiator that irradiates a sample with first excitation light having a first line width and second excitation light having a line width broader than the first line width; a spectroscopy measurer that, when first measurement light emitted from the sample when the sample is irradiated with the first excitation light and second measurement light emitted from the sample when the sample is irradiated with the second excitation light are incident, performs spectroscopy measurement on the first measurement light and the second measurement light; and a first selective optical system that has a first transmission band and a first stop band, and filters the first measurement light and the second measurement light incident on the spectroscopy measurer. The first excitation light and the second excitation light each have a main component in the first stop band, and the second excitation light has substantially no component in the first transmission band.

A spectroscopy system (10) for analyzing in-elastic scattered electromagnetic radiation from an object being irradiated by electromagnetic radiation is provided. The system comprises a tunable lens assembly (13) having a tunable lens provided in the beam path between an electromagnetic radiation source (11) and the object (0) and arranged to project a beam of electromagnetic radiation emitted from the electromagnetic radiation source onto an area of the object and receive and collimate the in-elastic scattered electromagnetic radiation from the object. Based on electromagnetic radiation detected by at least a first detector (121) a control unit (14) is capable making a decision to change the operational settings of the tunable lens.

Embodiments of the present invention provide a Raman spectroscopic inspection method, comprising the steps of: measuring a Raman spectrum of an object to be inspected successively to collect a plurality of Raman spectroscopic signals; superposing the plurality of Raman spectroscopic signals to form a superposition signal; filtering out a florescence interfering signal from the superposition signal; and identifying the object to be inspected on basis of the superposition signal from which the florescence interfering signal has been filtered out. By means of the above method, a desired Raman spectroscopic signal may be acquired by removing the interference caused by a florescence signal from a Raman spectroscopic inspection signal of the object. It may inspect correctly the characteristics of the Raman spectrum of the object so as to identify the object effectively.

Disclosed herein are methods and systems for analysis of samples. In some cases, the analysis may involve spectroscopic techniques such as Raman spectroscopy. The methods and systems may be used to identify one or more characteristics of a sample, such as the identity of, and the quantity of a molecule within the sample. The methods and systems may be used to determine an impurity of the sample, or inactive excipients within the sample. In some embodiments, the methods and systems of the present disclosure provide for rapid sample analysis that is more accurate, precise and cost-effective than traditional means.

A collection optics system for a spectrometer and a Raman spectral system including the collection optics system is provided. The collection optics system is configured to selectively collect a Raman signal from scattered light output from a target object, the collection optics system includes a non-imaging collection unit configured to collect the Raman signal and output the Raman signal, the non-imaging collection unit including an entrance surface on which the scattered light is incident and an exit surface through which the Raman signal is output, and a Raman filter provided on a portion of the entrance surface of the non-imaging collection unit and configured to block the scattered light including a fluorescence signal. Therefore, the collection optics system may suppress reception of the fluorescence signal of the scattered light and selectively collect the Raman signal.