A sample purification apparatus includes a container for separating, with a heavy solution, a mixed sample based on a specific gravity difference and a collector that collects a component in the mixed sample lighter in specific gravity than the heavy solution by receiving a supernatant flowed out from the container. The container includes a flow-out port provided in an uppermost portion of the container and a flow-out path that guides the supernatant flowed out through the flow-out port to the collector. the container has a horizontal cross-sectional area which gradually decreases upward starting from a prescribed height of the container to a height where the flow-out port is located.

A system or method for detecting an immune system activation state in a patient can include a sample preparation system configured to isolate white blood cells from a sample of the patient, a cytometry module configured to determine biophysical properties of the white blood cells of the sample, and an analysis module configured to analyze the biophysical properties.

A method and/or system can include processing a blood sample of a patient by degrading red blood cells of the blood sample using a lysing solution, quenching the degradation of the red blood cells after a threshold lysing time, centrifuging and aspirating the quenched solution to remove degraded red blood cell debris and concentrate white blood cells of the blood sample, and suspending the concentrated white blood cells in a buffer solution; within a threshold transfer time, deforming white blood cells, of the suspended white blood cells, within a microfluidic chip; and determining a probability that the patient is in an immune activation state based on images of the white blood cells acquired while deforming the white blood cells.

Devices, systems and methods are disclosed which relate to using a wet foam elution method for removal of particles from a flat filter. Particles are captured from the atmosphere onto the flat filter. The flat filter is then placed into an extractor which passes a stream of wet foam through the flat filter. Expansion of the foam works to efficiently remove captured particles. The foam flows from the filter along with the captured particles into a sample container. Once in the sample container, the foam quickly breaks down leaving an analysis ready liquid sample.

The present disclosure provides for acoustofluidic centrifuge systems that can enrich and separate nanoparticles disposed in a fluid, such as liquid droplets, in a fast and efficient manner. Exemplary systems include a sound wave generator, such as a pair of slanted interdigitated transducers, and a containment boundary, such as a PDMS ring. The sound wave generator can produce surface acoustic waves that are capable of driving droplets to spin in a manner that can separate different sized particles into groups. In some embodiments, the acoustofluidic centrifuge system can include a plurality of containment boundaries in fluid communication with each other, allowing particles to separate between the containment boundaries. Methods of operating such systems, including methods of isolating different exosome subpopulations, are also disclosed.

An apparatus and a method for isolating and/or preparing particles are provided.

A soil fractionation system can include a plurality of sample racks propelled by a drive system. Each sample rack can include a sample tube for holding a soil sample and a filter cup for receiving an extracted fraction of the soil sample. An extractor module of the fractionation system can include an extractor assembly and a filter assembly. A control system can control the relative positioning of the plurality of sample racks via the drive system, the relative movement between the extractor assembly and the sample tube, and the relative movement between the filter assembly and the filter cup.

A filter module (10) has a housing (12) which is subdivided by a membrane filter (14) into an inlet chamber (16), which is connected to an inlet connecting piece (22) arranged rigidly on the housing (12), and an outlet chamber (18), which has a filtrate outlet (20). The inlet connecting piece has two connectors, specifically a first connector (26) and a second connector (28), which connect selectively and fluidically to the inlet chamber with a 3-way valve (24) integrated into the inlet connecting piece. The valve has a first entry, which is connected to the first connector, a second entry, which is connected to the second connector, and an exit, which is connected to the inlet chamber. The first connector is configured as an adapter for outwardly sealed coupling of a culture medium bottle (30), which coupling permits a gravity-driven exchange of liquid with the first entry of the valve.

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation.

There is provided an apparatus for collecting/isolating cells from a sample including the target cells comprising: a membrane adapted to retain the target cells thereon, a first port in fluid communication with the membrane provided towards a first side of the membrane, a second port in fluid communication with the membrane provided towards a second side of the membrane, a sample container having at least one wall defining an interior space suitable to house the sample, provided on the first side of the membrane, a cell collection container provided towards the first side of the membrane, a filtrate container provided towards the second side of the membrane, a backwash fluid container provided towards the second side of the membrane, wherein the first port is movable between a first position wherein the first port is in fluid communication with the sample container, and a second position wherein the first port is in fluid communication with the cell collection container; and
wherein the second port is movable between a first position wherein the second port is in fluid communication with the filtrate container, and a second position wherein the second port is in fluid communication with the backwash fluid container.