The present invention discloses a bionic regulation method for a number of bounces of a droplet on a metal surface based on acoustic in-situ monitoring, which comprises the following steps: obtaining dynamic contact characteristics and acoustic properties of a solid-liquid interface of metal superhydrophobic surfaces with different surface information in the droplet bounce process based on an optical-acoustic synchronous in-situ testing system for droplet bounces; and obtaining a correlation among the metal superhydrophobic surface, the acoustic response and the droplet bounce behavior based on the dynamic contact characteristics and the acoustic properties of the solid-liquid interface, and machining and modifying a micro-nano structure of the metal surface by combining a coupled bionic concept according to the correlation. A scientific basis and a novel design method are provided for the bionic regulation of the number of droplet bounces over the metal surface.