A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

Various embodiments herein disclose a device, comprising one or more fluid interfacing components and a cartridge holder, wherein the one or more fluid interfacing components are fixed while the cartridge holder moves along a linear guide. Also disclosed herein are methods of using the device to analyze a sample containing particles, and methods of diagnosing a disease in a subject by using the device.

Various embodiments herein disclose a device, comprising one or more fluid interfacing components and a cartridge holder, wherein the one or more fluid interfacing components are fixed while the cartridge holder moves along a linear guide. Also disclosed herein are methods of using the device to analyze a sample containing particles, and methods of diagnosing a disease in a subject by using the device.

A device for facilitating volumetric blood-cell counting includes a blood reservoir configured to receive a blood sample, a first rotatable valve including a first duct having first and second ends, and a first container containing a first reagent. The first end of the first duct is configured to be selectively in fluid communication with the blood reservoir and first container. The device further includes a mixing chamber. The second end of the first duct is configured to be selectively in fluid communication with the mixing chamber.

A device for facilitating volumetric blood-cell counting includes a blood reservoir configured to receive a blood sample, a first rotatable valve including a first duct having first and second ends, and a first container containing a first reagent. The first end of the first duct is configured to be selectively in fluid communication with the blood reservoir and first container. The device further includes a mixing chamber. The second end of the first duct is configured to be selectively in fluid communication with the mixing chamber.

An outside opening of each aperture of a plurality of counting chambers for performing particle counting based on the electric resistance method is connected to suction pump through a confluent piping. Liquid supplying part supplies an additional liquid to the counting chamber side after completion of counting of counting chamber, so that the liquid level of sample liquid of counting chamber will not descend to aperture or a predetermined liquid level.

An outside opening of each aperture of a plurality of counting chambers for performing particle counting based on the electric resistance method is connected to suction pump through a confluent piping. Liquid supplying part supplies an additional liquid to the counting chamber side after completion of counting of counting chamber, so that the liquid level of sample liquid of counting chamber will not descend to aperture or a predetermined liquid level.

The present invention provides a classification analysis method, a classification analysis device, and a storage medium for classification analysis, which enable, with high accuracy, the classification analysis of particulate or molecular analytes. As a means for solving the problem, a data group of particle-passage detection signals is based which are detected by a nanopore device 8 in accordance with passage of subject particles through a through-hole 12. A feature value is obtained in advance which indicates the feature of the waveform of the pulse signals corresponding to the passage of the predetermined analyte and the feature value obtained in advance is set as the learning data for the machine learning. The feature value obtained from the pulse signals of said analyzed data is set as a variable and the classification analysis on the predetermined analytes in the analyzed data can be performed by executing a classification analysis program due to the machine learning.

The present disclosure provides a particle analyzer and a particle test control method and device thereof. A method comprises, acquiring a blood sample in a test location; preparing a diluted sample by the acquired sample; after acquiring a diluted sample, monitoring whether a pore blocking event occurs during a counting process; when the pore blocking event occurs, suspending the test of the sample, and performing an unblocking operation; and after the unblocking operation is completed, re-counting the same diluted sample without re-acquiring and re-diluting the blood sample by the impedance method after the unblocking operation.

The present disclosure provides a particle analyzer and a particle test control method and device thereof. A method comprises, acquiring a blood sample in a test location; preparing a diluted sample by the acquired sample; after acquiring a diluted sample, monitoring whether a pore blocking event occurs during a counting process; when the pore blocking event occurs, suspending the test of the sample, and performing an unblocking operation; and after the unblocking operation is completed, re-counting the same diluted sample without re-acquiring and re-diluting the blood sample by the impedance method after the unblocking operation.