One aspect of this disclosure relates to a computer-implemented method for determining a force acting on at least part of a structure, for example a biological structure, such as a DNA molecule. The method comprises controlling a light-sensitive system, e.g. of a microscope, to determine light information based on light from the structure. The light is incident on at least a part of the light sensitive system. The light-sensitive system may be said to capture the light from the structure. The at least part of the structure comprises one or more optically active entities, such as DNA intercalator molecules and donor/acceptor fluorophores. At least one of (i) an optical activity of the entities and (ii) a quantity of the entities depends on the force acting on the at least part of the structure. Furthermore, the light information defines a light property value associated with said at least part of the structure. The method further comprises determining the force acting on the at least part of the structure on the basis of said light property value and a reference light property value.

The present technology provides a technology for stabilizing break-off timings. Therefore, according to the present technology, there is provided a microparticle analysis device or the like including at least: a flow path in which a fluid including a sample flow containing microparticles and a sheath flow flowing to contain the sample flow; a droplet formation unit configured to form a droplet in the fluid by imparting vibration to the fluid using a vibration element; an electric charge application unit configured to apply electric charge to a droplet containing the microparticles; an imaging unit configured to obtain a photo of a phase of a certain time; and a control unit configured to control a timing at which the droplet breaks off on a basis of the photo.

Disclosed herein are systems and methods capable of identifying, tracking, and sorting particles flowing in a channel, for example, a microfluidic channel having a fluid medium. The channel and the fluid medium can have a similar refractive index such that they appear translucent or transparent when illuminated by electromagnetic radiation. The particles can have a refractive index substantially different from that of the channel and the medium, such that the particles interfere with the electromagnetic radiation. A sensor can be disposed adjacent to the channel to record the electromagnetic radiation. The sensor can be used for identifying, tracking, and sorting the particles.

Disclosed herein include systems, devices, methods, and spillover editor for displaying and editing spillover values. A view of a spillover editor can comprise a triangular grid of rows and columns, representing flourophores, each comprising at least one display area and two spillover values. After receiving an adjusted spillover value, an adjusted view of the spillover editor can comprise adjusted plots determined using the adjusted spillover value.

[Object] To provide an information processing apparatus, a particle sorting system, a program, and a particle sorting method that practice a spectral type analysis usable for sorting particles. [Solving Means] The information processing apparatus according to an aspect of the present technology includes: an analysis unit; a learning unit; and a discrimination unit. The analysis unit calculates fluorophore information indicating respective amounts of luminescence of a plurality of types of fluorophores on the basis of detection data indicating amounts of luminescence of fluorescence at respective wavelength bands, the fluorescence having been emitted from a particle irradiated with excitation light, discriminates whether or not to treat the particle as a process target in accordance with the fluorophore information, and generates teaching data by associating a result of the discrimination with the detection data. The learning unit applies a machine learning algorithm to the teaching data, learns a characteristic of the detection data discriminated as the process target, and generates dictionary data including a result of the learning. The discrimination unit discriminates whether or not the particle whose detection data has been acquired is the process target on the basis of the dictionary data when the detection data is supplied.

Flow cytometry investigation and flow cytometry result analysis to determine an optimized dilution factor range for flow cytometry investigation of a target sample fluid stock using a flow cytometer. The optimized dilution factor range may be determined using a screening assay module of a flow cytometry system to analyze flow cytometry results determined to fall within a dynamic range of a flow cytometer to determine the optimized dilution factor range for a given flow cytometer to investigate a given target sample fluid stock. In turn, a titer assay module may be executed to prepare particle titer results based on optimized target fluid samples provided within the optimized dilution factor range. The screening assay module and/or the titer assay module may provide automated data processing with data notifications and confirmations to provide robust data analysis.

A flow cytometer module configured to be integrated with a liquid handling system is provided herein. The flow cytometer module includes (a) a flow cell, (b) a first fluidic pathway, (c) an inlet configured to receive a sample introduction device of the liquid handling system including one or more samples, (d) a second fluidic pathway in fluid communication with the first fluidic pathway, (e) a laser interrogation device configured to examine the one or more samples at a laser interrogation point in the second fluidic pathway, and (f) a controller in communication with the liquid handling system and configured to cause the flow cytometer module to perform functions comprising: (i) recording data from the laser interrogation device corresponding to a plurality of events as the one or more samples pass the laser interrogation point, and (ii) transmitting the data corresponding to the plurality of events to the liquid handling system.

A system for processing a substrate is provided. The system includes a process chamber including one or more sidewalls enclosing a processing region; and a substrate support. The system further includes a passageway connected to the process chamber; and a first particle detector disposed at a first location along the passageway. The first particle detector includes an energy source configured to emit a first beam; one or more optical devices configured to direct the first beam along one or more paths, where the one or more paths extend through at least a portion of the passageway. The first particle detector further includes a first energy detector disposed at a location other than on the one or more paths. The system further includes a controller configured to communicate with the first particle detector, wherein the controller is configured to identify a fault based on signals received from the first particle detector.

Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.

To provide a technology for increasing the speed of sample imaging using a microscope. The present technology provides a flow channel structure including an imaging flow channel in which a fluid containing imaging targets flows in the same direction as the optical axis of an objective lens. The present technology also provides an imaging member including the flow channel structure. The present technology also provides an imaging method that includes imaging an imaging target in an imaging flow channel in which a fluid containing the imaging target flows in the same direction as the optical axis of an objective lens. The present technology also provides an imaging target analysis device and an imaging target analysis system that include the imaging member.