A counting method includes aggregating particles in a sample by action of first-direction dielectrophoretic force, dispersing the aggregated particles by action of second-direction dielectrophoretic force, which is different from the first-direction dielectrophoretic force, capturing a dispersion image including the dispersed particles, and determining the number of particles on the basis of the dispersion image.

A system and method for automated testing a sample of a body fluid for the presence of bacteria is described. The system includes a fluid handling device, Incubator, flow cytometer, at least a processor, and a memory configuring the at least a processor to distribute a portion of the plurality of fluid samples within a well plate to at least a first well, divide the portion of the plurality of fluid samples from the at least a first well into at least two wells including a T.sub.0 well and a T.sub.1 well, obtain a T.sub.0 enumerative baseline bacterial value at time T.sub.0, culture the fluid samples in the T.sub.1 well using the incubator, obtain a T.sub.1 enumerative control bacterial value at time T.sub.1, and determine a presence of bacteria as a function of the T.sub.0 enumerative baseline bacterial value and the T.sub.1 enumerative control bacterial value.

A high-power-microscope-assisted identification method of maize haploid plants is provided, the method is implemented by a device including a high power microscope, a main frame disposed on an objective table of the high power microscope and a computer and includes four procedures of sample information input, automatic testing of a batch of samples, automatic analysis and comparison, and automatic generation of data results. Vertical sliding grooves are symmetrically formed in the main frame, and a vertical supporting plate is disposed at an upper end of the main frame. Horizontal sliding grooves are symmetrically formed in the vertical supporting plate, and a horizontal supporting plate is disposed on the vertical supporting plate.

Aspects of the present disclosure include methods for determining photodetector gain for a plurality of photodetectors in a light detection system. Methods according to certain embodiments include applying a reference voltage to each photodetector in the light detection system, generating a reference data signal for each photodetector at the reference voltage, irradiating with a light source the photodetectors at a plurality of different applied voltages, generating output data signals for each photodetector at each of the plurality of different voltages and calculating gain of the photodetectors at each of the plurality of different applied voltages based on the output data signals for each photodetector at each applied voltage and the reference data signal. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a plurality of photodetectors for practicing the subject methods are also described. Non-transitory computer readable storage medium are also provided.

A particle detection device includes a detection tube, a light emitter, a light receiver, and a processing unit. The detection tube is for a detection solution to pass through. The light emitter generates a detection light and emits the detection light to the detection solution. The light receiver receives the detection light scattered from the detection solution. The processing unit generates a received light intensity value according to the detection signal generated by the light receiver, and determines whether the received light intensity value is greater than a first threshold value: if greater, generating a detection result of particles; otherwise, generating a detection result of no particles. Then it provides a basis for semiconductor manufacturing companies to evaluate whether the detection solution can be used in a high-precision manufacturing processes, thereby optimizing the manufacturing process and improving the yield rate of the high-precision manufacturing process.

Systems and methods are provided for Quantitative Phase Microscopes (QPM) having laser systems including one or more of laser scissors and laser tweezers. In one embodiment, the system includes one or more structural elements, such as a stage and dichroic plate for operation of a QPM with laser scissors/tweezers. Another embodiment is directed to a method of operating a QPM system having laser scissors/tweezers. One or more solutions are provided for biodmedical applications of a QPM system including simulation and analysis of trauma on cellular structures and organelles. Processes are also provided for simulation and analysis of traumatic injury, including imaging and analysis of astrocytes.

An exemplary mobile impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. The mobile cytometer may be controlled by a computer (e.g., smartphone) application. Calibration of the portable device may be performed using a component of known impedance value. With the developed portable flow cytometer, analysis may be performed on two sickle cell samples and a healthy cell sample. The acquired results may subsequently be analyzed to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals may be observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.

There is provided a technology that supports selection of a label to be used for analysis of target molecules. The present technology provides a label selection support system including an information acquisition unit that obtains, via a network, information associated with a plurality of target molecules to be analyzed, an information processor that obtains, using the information associated with a plurality of target molecules, in vivo expression information of the plurality of target molecules from a database storing in vivo expression information of target molecules and generates support information associated with assignment of a label to each of the plurality of target molecules on the basis of the expression information, and a transmitter that transmits the generated support information via the network.

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

A method for enhancing vacuum tolerance of optical levitation particles includes steps of: (1) turning on a trapping laser to form an optical trap, loading the particles to an effective capture region of the optical trap, and collecting scattered light signals; (2) turning on the preheating laser, and directing a preheating laser beam to the captured particles; (3) adjusting a power of the preheating laser until a particle heating rate is larger than a heat dissipation rate; (4) turning on the vacuum pump, and stopping evacuating when a vacuum degree is greater than a vacuum inflection point of a first reduction of the effective capture region of the optical trap; and (5) turning off the preheating laser when the scattered light signals collected by the photodetector no longer changes. The present invention improves a stable capture probability of the particles in high vacuum environment.