A photoacoustic flow cytometry (PAFC) device for the in vivo detection of cells circulating in blood or lymphatic vessels is described. Ultrasound transducers attached to the skin of an organism detect the photoacoustic ultrasound waves emitted by target objects in response to their illumination by at least one pulse of laser energy delivered using at least one wavelength. The wavelengths of the laser light pulse may be varied to optimize the absorption of the laser energy by the target object. Target objects detected by the device may be unlabelled biological cells or cell products, contrast agents, or biological cells labeled with one or more contrast agents.

The present disclosure relates to a spectral sensor for detection of individual light-emitting particles. The sensor is comprising an array of photo-sensitive detectors for detecting light emitted by said individual light-emitting particles and a filter array comprising a plurality of different band-stop filters. The filter array is configured to transmit wavelengths in a detectable wavelength region to the array of photo-sensitive detectors, and wherein each band-stop filter is associated with one or more particular photo-sensitive detectors, and the plurality of different band-stop filters are configured to reflect different wavelength intervals within said detectable wavelength region so that each photo-sensitive detector of the array is configured to detect the wavelengths of the detectable wavelength region other than the reflected wavelength interval of the band-stop filter being associated with the photo-sensitive detector. The sensor is further comprising a processing unit in communication with said array of photo-sensitive detectors and configured for determining a spectral characteristic of an individual light-emitting particle based on the response from said array of photo-sensitive detectors.

A particle monitoring system may include a volume for containing a fluid in which particles are suspended, a photosensitive layer, a light encoding layer sandwiched between the volume and the photosensitive layer and electrodes to apply an electric field to the fluid within the volume and proximate the photosensitive layer.

A method of selecting a type of particle for use in standardisation and/or calibration of a flow cytometer. The method includes determining the location of two or more particle focal points of particles flowing through a cross section of a channel in the flow cytometer; for each type of particle, determining for each particle focal point, for a beam of light directed at a type of particle at said particle focal point from a first direction, the total intensity of light scattered along a second direction; determining the difference between the highest and lowest determined light intensities of the light intensities determined at the two or more particle focal points; and selecting a type of particle for which the difference between the highest and lowest determined light intensities at the two or more particle focal points is below a predetermined threshold.

Disclosed herein are devices, systems, and methods for sorting a particle based on a characteristic of a particle.

A particle component analyzing device is provided. The particle component analyzing device comprises: a catching body which catches a particle in an aerosol which is subject to measurement, an energy beam irradiating unit which irradiates an energy beam to the particle which is caught by the catching body, and an analyzer which analyzes at least any of a component and an amount of the particle based on a desorbed component of the particle which is desorbed from the catching body by irradiation of the energy beam, wherein the catching body has a temperature measuring unit, the particle component analyzing device further comprising a controlling unit which controls an output of the energy beam irradiating unit based on a temperature of the catching body which is measured by the temperature measuring unit.

A system for orienting particles in a microfluidic system includes one or more radiation pressure sources arranged to expose particles to radiation pressure to cause the particles to adopt a particular orientation in the fluid. A system for sorting particles in a microfluidic system includes a detection stage arranged to detect at least one difference or discriminate between particles in the fluid flow past the detection stage, and one or more radiation pressure sources past which the particles move sequentially and a controller arranged to switch radiation energy to cause a change in direction of movement of selected particles in the fluid flow to sort the particles. The particles may be biological particles such as spermatazoa. The radiation pressure may be optical pressure and may be from one or more waveguides which may extend across a channel of the microfluidic system.

Provided are a microparticle sorting device, and a method and a program for sorting microparticles capable of stabilizing sorting performance over a prolonged period of time. The microparticle sorting device includes an imaging element and a controller. The imaging element obtains an image of fluid and fluid droplets at a position where the fluid discharged from an orifice which generates a fluid stream is converted into the fluid droplets. The controller controls driving voltage of an oscillation element which gives oscillation to the orifice and/or controls a position of the imaging element based on a state of the fluid in the image and/or a state of a satellite fluid droplet. The satellite fluid droplet does not include microparticles and exists between the position, where the fluid is converted into the fluid droplets, and a fluid droplet, among fluid droplets including the microparticles, which is closest to the position where the fluid is converted into the fluid droplets.

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements.

A vehicular dust sensor may include a case having an inner space, a light source module disposed in the inner space of the case, and irradiating an air passage with light, the air passage being provided in the light source module for introduction and pass of air and dust, a condensing lens to collect light, emitted from the light source module and scattered by dust in air in the air passage, a light receiving module receiving the scattered light collected by the condensing lens to output an electric signal corresponding to a dust concentration, and an inner housing disposed in the inner space of the case to form the air passage.