An analysis device includes an analysis unit configured to receive scattered light, transmitted light, fluorescence, or electromagnetic waves from an observed object located in a light irradiation region light-irradiated from a light source and analyze the observed object on the basis of a signal extracted on the basis of a time axis of an electrical signal output from a light-receiving unit configured to convert the received light or electromagnetic waves into the electrical signal.

A system for characterizing at least one particle from a fluid sample is disclosed. The system includes a filter disposed upstream of an outlet, and a luminaire configured to illuminate the at least one particle at an oblique angle. An imaging device is configured to capture and process images of the illuminated at least one particle as it rests on the filter for characterizing the at least one particle. A system for characterizing at least one particle using bright field illumination is also disclosed. A method for characterizing particulates in a fluid sample using at least one of oblique angle and bright field illumination is also disclosed.

A single-particle localization microscope, including an optical system configured to illuminate a sample region with a sequence of light patterns having spatially different distributions of illumination light adapted to cause a single particle located in the sample region to emit detection light, a detector configured to detect a sequence of intensities of the detection light emerging from the sample region in response to the sequence of illuminating light patterns, and a processor configured to determine, based on the sequence of intensities of the detection light, an arrangement of potential positions for locating the particle. The processor further illuminates the sample region with at least one subsequent light pattern, causes detection of at least one subsequent intensity, and decides, based on the at least one subsequent intensity of the detection light, which one of the multiple potential positions represents an actual position of the particle in the sample region.

Described herein are apparatus and methods for orthographic imaging of particles. Particularly, a method to obtain contact-free images of aerosol particles with digital holography from three orthogonal directions is described and demonstrated. Diode lasers of different wavelengths simultaneously illuminate free flowing particles to form holograms on three sensors. Images of the particles are reconstructed from the holograms and used to infer the three-dimensional structure of single spherical particles or clusters of sphere-like particles. The apparatus employs inexpensive components and requires no lenses to achieve the imaging, which gives it a large sensing volume and simple design.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

The present invention is concerned with a method for detection of presence and/or quantities of silver nanoparticles in a specimen. The method includes the steps of a) providing a detection organism suspended in a medium, b) treating the detection organism with zinc ions thus effecting auto-fluorescence therefrom, and then measuring degree of fluorescence of the detection organism suspended medium, c) adding the specimen to the detection organism suspended medium, treating the detection organism therein with the specimen for a period of time, and measuring change of fluorescence of the detection organism-suspended medium over time, d) calculating amount of silver ions intracellularly dissolved from the silver nanoparticles and accumulated in the detection organism in view of the change of fluorescence, and e) extrapolating quantity of silver nanoparticles in the specimen in view of the change of fluorescence and the amount of the intracellularly dissolved silver ions.

A particle detection system may include a light source, a first beam splitter, a particle interrogation zone, a reflecting surface, a second beam splitter, a first photodetector, and a second photodetector. The first beam splitter may be configured to split the source beam into an interrogation beam and a reference beam. The particle interrogation zone may be disposed in the path of the interrogation beam. The reflecting surface may be configured to reflect the interrogation beam back on itself. The second beam splitter may be configured to: (i) receive the reference beam and side scattered light from one or more particles interacting with the interrogation beam in the particle interrogation zone; and (ii) produce a first component beam and second component beam. The first photodetector may be configured to detect the first component beam. The second photodetector may be configured to detect the second component beam.

A photo-electric smoke detector assembly includes a Y-shaped optic block with a first photo-electronic device mounted on a first end and second and third photo-electronic devices mounted on a second end, with an interaction volume at a midpoint. Two of the photo-electronic devices direct pulses of differing wavelengths toward the interaction volume, and the remaining photo-electronic device receives light from the pulses scattered off of smoke particles in the interaction volume.

The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles).

The invention relates to a method for detecting a particle in a container filled with liquid, the method having the following steps: dispensing a liquid sample into the container, scanning a partial volume area of the container in order to detect a particle located in the liquid sample, characterized in that an upper limit and a lower limit of the partial volume area is determined in a calibration operation upstream of the dispensing process.