Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

A cell detection method and a cell detection device. The cell detection method includes: dividing a liquid sample into a plurality of droplets in a sample detection region so that each of the plurality of droplets includes fewer than ten cells; and performing optical detection on the plurality of droplets in the sample detection region to determine a target droplet including a target cell from the plurality of droplets.

A microfluidic analyser and a method of using the same is disclosed. The microfluidic analyser comprising a droplet generator, an analyte flow channel in fluid communication with said droplet generator at a first end, wherein said flow channel is configured to allow the droplets to flow in from the first end and exit from a second opposing end, said flow channel receiving at least one illumination channel positioned at a predetermined location between the first and the second end to excite contents of the droplets and said flow channel further comprising a plurality of receiving channels set at predetermined angles to an axis of the flow channel to interrogate at least one optical signal from the illuminated droplet traversing the flow channel and wherein said receiving channels terminate in a signal detector at the distal end away from the flow channel.

Described herein are microfluidic devices and methods that can greatly improve cell quality, streamline workflows, and lower costs. Applications include research and clinical diagnostics in cancer, infectious disease, and inflammatory disease, among other disease areas.

Apparatus for performing multiple different measurements on a small specimen sample, enabling testing and diagnoses in real time at the point of care are described. The core of the apparatus includes an ultrasonic resonator cavity where acoustic resonances are used to determine the speed of sound and sound attenuation in a single droplet. Acoustic measurements are made in the reflection mode using electrical impedance of a small piezoelectric crystal transducer that operates in the thickness longitudinal mode. Combination of this technology with electromagnetic, electrical, and magnetic fields permits multiple types of measurements to be made using the same resonator cavity.

Provided is a biomaterial removing device including an air injection part, a first processing part spaced apart from the air injection part, and a second processing part spaced apart from the air injection part with the first processing part therebetween, wherein the first processing part includes a first biomaterial removing part configured to remove biomaterials included in air collected from the air injection part and a first monitoring part, and the second processing part includes a second biomaterial removing part configured to remove the residual biomaterials and a second monitoring part, wherein the first biomaterial removing part includes a dry air purifier, the second biomaterial removing part includes a wet air purifier, and the first biomaterial removing part and the second biomaterial removing part each include an image sensor.

The invention relates to the field of biotechnology and pharmaceutics. Proposed is a method for ultra-high performance screening of biological objects which is based on microfluidic generation of droplets of a biocompatible water-in-oil-in-water double emulsion, and also a method for producing a monodisperse biocompatible water-in-oil-in-water double emulsion. The invention can be used in diagnosing conditions and diseases in mammals, as well as for investigating biological objects.

Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. A contrast pattern for autofocusing is provided on the flowcell, for example at an edge of a rear illumination opening. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Blood cell images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.

Compositions and methods for identifying antigen-specific T cells, including determining paired T cell receptor sequences for a specific antigen, are described. Compositions and methods for identifying neoantigen-specific T cells are also described. Microfluidic devices useful for identifying antigen-specific T cells, and methods of using the same, are also described.

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.