An imaging system for a biological sample includes a sample container having at least one biological cell that is in contact with an interface surface of a container interface. The imaging system also includes illuminating optics that output a light beam aligned with a sample plane, the light beam being oriented horizontally along a transverse (XY) plane and illuminating the biological cell vertically along an axial (XZ) plane. The imaging system further includes imaging optics aligned horizontally along the transverse (XY) plane with the interface in the sample container, the imaging optics being configured to detect along the axial (XZ) plane a magnified image of a measurable contact angle between the biological cell and the interface surface. The measurable contact angle changes over time and is indicative of biological adhesion between the biological cell and another biological cell.

A fluidic device including a substrate having a well array that includes regularly arranged wells that have a same shape and are open to a surface of the substrate, and a cover member facing the well array. The well array and the cover member are positioned to have a space therebetween, which forms a flow path through which a fluid flows, and the wells including a well A and a well B closest to the well A satisfy formula (1): 0.8≤Da/Dab<1 . . . (1) where Dab is a distance between a centroid Ca of an opening of the well A and a centroid Cb of an opening of the well B, and Da is a diameter of a circle having a same area as the opening of the well A.

Provided is a test chip capable of significantly suppressing unevenness in the color development. The test chip includes a sheet shape and includes a first layer on a front side and a second layer on a back side, wherein the first layer and the second layer are located adjacent to each other. One of the first layer and the second layer has a liquid receiving section A. The first layer has at least a detection confirmation section B. The second layer has at least a liquid flow section D adjacent to the detection confirmation section B and a liquid passage E connected to the liquid flow section D. The test chip is configured such that, in case where the liquid receiving section A is provided in the first layer, the liquid receiving section A is spaced from the detection confirmation section B and a test liquid dropped into the liquid receiving section A passes through the liquid receiving section A, the liquid passage E, and the liquid flow section D in the stated order, by means of capillary action, and flows to the detection confirmation section B.

Multi-directional microfluidic devices and methods for using the same are provided. Aspects of the present disclosure include microfluidic devices that include a chamber having a separation medium, a first binding medium, and a second binding medium. In addition, the devices include a flow field element configured to subject the chamber to two or more directionally distinct flow fields. Methods of using the devices, as well as systems and kits that include the devices are also provided. The devices, systems and methods find use in a variety of different applications, including diagnostic, research and validation assays.

Reusable network of spatially-multiplexed microfliuidic channels each including an inlet, an outlet, and a cuvette in-between. Individual channels may operationally share a main or common output channel defining the network output and optionally leading to a disposable storage volume. Alternatively, multiple channels are structured to individually lead to the storage volume. An individual cuvette is dimensioned to substantially prevent the formation of air-bubbles during the fluid sample flow through the cuvette and, therefore, to be fully filled and fully emptied. The overall channel network is configured to spatially lock the fluidic sample by pressing such sample with a second fluid against a closed to substantially immobilize it to prevent drifting due to the change in ambient conditions during the measurement. Thereafter, the fluidic sample is flushed through the now-opened valve with continually-applied pressure of the second fluid. System and method for photometric measurements of multiple fluid samples employing such network of channels.

Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

The present disclosure provides microfluidic flow channel structure, detection system and method for using detection system, aiming at improving uniformity of liquid introduction of microfluidic detection system. Microfluidic flow channel structure includes liquid inlet section (1), main chamber (3) and liquid outlet section (2), main chamber (3) includes liquid inlet end (31), chamber middle part (33) and liquid outlet end (32); liquid inlet section (1), liquid inlet end (31), chamber middle part (33), liquid outlet end (32) and liquid outlet section (2) are sequentially connected together; width of liquid inlet end (31) is gradually increased in direction from liquid inlet section (1) to chamber middle part (33); thinning flow guidance region (310) is provided at edge of liquid inlet end (31) formed as width of liquid inlet end varies, and has thickness less than that of remaining region except thinning flow guidance region (310) of main chamber (3).

The present invention provide compositions and methods for classifying leukocytes in a leukocyte population using fluorescence detection. The methods include contacting a leukocyte population in a sample having one or more leukocyte types with a diagnostic composition, exciting the leukocyte population with a light source; and measuring emitted light from each of the one or more leukocyte types to classify the leukocyte population.

The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

The present invention relates to devices and methods for the qualitative and/or quantitative detection of particles. In particular, the invention relates to devices for the detection of particles, comprising a reaction chamber formed within a chamber body between a first surface and a second surface, wherein the second surface is located opposite to the first surface, and one or more displacers, wherein the distance between the first surface and the second surface is variable via the one or more displacers at least in one or more parts of the surface area of the first surface and/or second surface. The invention also relates to corresponding methods for the detection of particles.