The invention relates to a system (100) for monitoring deviations of a state of a cell culture in a bioreactor (104, 106) from a reference state of a cell culture in a reference bioreactor (102). The bioreactor comprises the same medium (M1) as the reference bioreactor. The system comprises: •—a storage medium (114) comprising: •a PACO-reference profile (116) indicative of a deviation of a CO2 off gas rate (ACO.sub.R-M-.sub.ti) measured in the reference bioreactor from a predicted CO2 off gas rate (ACO.sub.R-EXP-ti) of the reference bioreactor; •a data object comprising a medium-specific relation (136) between the pH value of the medium (M1) and a respective fraction of CO2 gas in a gas volume when said medium is in pH-CO2 equilibrium state with said gas volume and lacks the cell culture; •—an interface (128) for receiving (212) a current CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.t i) and a current pH value (pH.sub.Bi-ti) of the medium of the bioreactor (104, 106); •—a comparison unit (130) configured for computing (214, 216): •a PACO value (PACO.sub.B1-tir PACO.sub.Bi-ti) the PACO-value being indicative of a deviation of a CO2 off gas rate (ACO.sub.Bi-M-ti, ACO.sub.B2-M-.sub.ti) measured in the bioreactor from a predicted CO2 off gas rate (ACO.sub.B1-EXP-ti, ACO.sub.B2-E xp-.sub.t i). a difference between the computed PACO value (PACO.sub.Bi-ti, PACO.sub.B2-ti) and a respective reference PACO value (PACO.sub.R-ti) in the PACO-reference profile (116).

Methods, apparatuses and systems for providing gas detecting apparatuses (e.g., electrochemical detectors) are disclosed herein. An example gas detecting apparatus may comprise a sensing component comprising: a housing configured to receive a sample gaseous substance comprising a target gaseous substance and an interferent gaseous substance; a reference electrode, disposed within the housing; a counter electrode disposed within the housing; and a sensing electrode disposed within the housing that is operatively coupled to a bias voltage circuit.

Methods, apparatuses and systems for providing gas detecting apparatuses (e.g., electrochemical detectors) are disclosed herein. An example gas detecting apparatus may comprise a sensing component comprising: a housing configured to receive a sample gaseous substance comprising a target gaseous substance and an interferent gaseous substance; a reference electrode, disposed within the housing; a counter electrode disposed within the housing; and a sensing electrode disposed within the housing that is operatively coupled to a bias voltage circuit.

Various example embodiments described herein relate to an electrochemical gas sensor. The electrochemical gas sensor can include a sensor cap having one or more solid features disposed on a surface of the sensor cap. The electrochemical gas sensor can include a counter electrode configured to generate a gas during use of the electrochemical gas sensor. The electrochemical gas sensor can include a vent assembly adapted to release at least a portion of the gas generated at the counter electrode out from the electrochemical gas sensor. The vent assembly can include a vent conduit and a vent membrane that defines a passage for the gas to flow from an extended portion of the counter electrode, to the vent conduit, via the vent membrane, so as to be vented from the electrochemical gas sensor.

Various example embodiments described herein relate to an electrochemical gas sensor. The electrochemical gas sensor can include a sensor cap having one or more solid features disposed on a surface of the sensor cap. The electrochemical gas sensor can include a counter electrode configured to generate a gas during use of the electrochemical gas sensor. The electrochemical gas sensor can include a vent assembly adapted to release at least a portion of the gas generated at the counter electrode out from the electrochemical gas sensor. The vent assembly can include a vent conduit and a vent membrane that defines a passage for the gas to flow from an extended portion of the counter electrode, to the vent conduit, via the vent membrane, so as to be vented from the electrochemical gas sensor.

A moisture regulating electrochemical sensor includes a sensor electrolyte housed in a chamber of a casing configured with electrodes connected electrically to the sensor electrolyte and configured to be connected electrically to an exterior circuit, an inlet, a vent, and a membrane permeable only to moisture, the chamber between the inlet and the vent each to the sensor electrolyte in the chamber, the inlet allowing a target gas to pass through from an exterior target gas environment to the sensor electrolyte in the chamber, and the membrane operatively coupled to the vent, allowing only moisture to translate through the vent between an exterior vent environment and the sensor electrolyte in the chamber.

A moisture regulating electrochemical sensor includes a sensor electrolyte housed in a chamber of a casing configured with electrodes connected electrically to the sensor electrolyte and configured to be connected electrically to an exterior circuit, an inlet, a vent, and a membrane permeable only to moisture, the chamber between the inlet and the vent each to the sensor electrolyte in the chamber, the inlet allowing a target gas to pass through from an exterior target gas environment to the sensor electrolyte in the chamber, and the membrane operatively coupled to the vent, allowing only moisture to translate through the vent between an exterior vent environment and the sensor electrolyte in the chamber.

A method of using mass and force indicators in electrochemical gas sensor management is provided. The method includes measuring a change in mass of an electrochemical gas sensor and determining a status of the electrochemical gas sensor based on results of the measuring of the change in mass.

A method of forming a glass electrochemical sensor is described. In some embodiments, the method may include forming a plurality of electrical through glass vias (TGVs) in an electrode substrate; filling each of the plurality of electrical TGVs with an electrode material; forming a plurality of contact TGVs in the electrode substrate; filling each of the plurality of contact TGVs with a conductive material; patterning the conductive material to connect the electrical TGVs with the contact TGVs; forming a cavity in a first glass layer; and bonding a first side of the first glass layer to the electrode substrate.

A method of forming a glass electrochemical sensor is described. In some embodiments, the method may include forming a plurality of electrical through glass vias (TGVs) in an electrode substrate; filling each of the plurality of electrical TGVs with an electrode material; forming a plurality of contact TGVs in the electrode substrate; filling each of the plurality of contact TGVs with a conductive material; patterning the conductive material to connect the electrical TGVs with the contact TGVs; forming a cavity in a first glass layer; and bonding a first side of the first glass layer to the electrode substrate.