A device for separating analytes is disclosed. The device has a sample injector, sample injection needle, sample reservoir container in communication with the sample injector, chromatography column downstream of the sample injector, and fluid conduits connecting the sample injector and the column. The interior surfaces of the fluid conduits, sample injector, sample reservoir container, and column form a flow path having wetted surfaces. A portion of the wetted surfaces of the flow path are coated with an alkylsilyl coating that is inert to at least one of the analytes. The alkylsilyl coating has the Formula I:

##STR00001## R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, and R.sup.6 are each independently selected from (C.sub.1-C.sub.6)alkoxy, —NH(C.sub.1-C.sub.6)alkyl, —N((C.sub.1-C.sub.6)alkyl).sub.2, OH, OR.sup.A, and halo. R.sup.A represents a point of attachment to the interior surfaces of the fluidic system. At least one of R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, and R.sup.6 is OR.sup.A. X is (C.sub.1-C.sub.20)alkyl, —O[(CH.sub.2).sub.2O].sub.1-20, -(C.sub.1-C.sub.10)[NH(CO)NH(C.sub.1-C.sub.10)].sub.1-20-, or -(C.sub.1-C.sub.10)[alkylphenyl(C.sub.1-C.sub.10)alkyl].sub.1-20-.

A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I:

##STR00001##

R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, and R.sup.6 are each independently selected from (C.sub.1-C.sub.6)alkoxy, —NH(C.sub.1-C.sub.6)alkyl, —N((C.sub.1-C.sub.6)alkyl).sub.2, OH, OR.sup.A, and halo. R.sup.A represents a point of attachment to the interior surfaces of the fluidic system. At least one of R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, and R.sup.6 is OR.sup.A. X is (C.sub.1-C.sup.20)alkyl, —O[(CH.sub.2).sub.2O].sub.1-20-, —(C.sub.1-C.sub.10)[NH(CO)NH(C.sub.1-C.sub.10)].sub.1-20-, or —(C.sub.1-C.sub.10)[alkylphenyl(C.sub.1-C.sub.10)alkyl].sub.1-20-.

The present disclosure is directed at the selection and design of columns for liquid chromatography including liquid chromatography devices and systems and corresponding methods of operation, particularly in the field of high pressure liquid chromatography (HPLC).

One object of the present invention is to prevent non-specific metal coordination adsorption of solutes in a sample, while achieving high pressure resistance through the use of a metal column hardware column, and to prevent non-specific adsorption of basic solutes and non-specific hydrophobic adsorption of highly hydrophobic solutes. As a means for achieving the foregoing, proposed is column hardware 1 for liquid chromatography, in which a first membrane 41 containing SiO.sub.2 as a main component is provided on a metallic liquid-contact portion by mobile phase 3 of the column hardware 1 for liquid chromatography, and a second membrane 42 in which silanol groups on the SiO.sub.2 surface are alkylsilylated is provided on a surface 411 of the first membrane 41.

A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of a vapor deposition coated frit together with an uncoated frit in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having a single coated frit within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

The present disclosure provides devices, systems, kits and methods useful for quantitation of biomolecules such as intact proteins and nucleic acids.

In an aspect, a method for forming a microcolumn comprises steps of: (a) providing a sacrificial fiber; (b) forming a microcolumn body around said sacrificial fiber; and (c) removing said sacrificial fiber from said microcolumn body such that a hollow channel is formed within said microcolumn body via removal of said sacrificial fiber. In any embodiment of the methods disclosed herein for forming a microcolumn, said hollow channel extends through said microcolumn body and is continuous between a first end and a second end. The first end may be an inlet and the second end may be an outlet, for example, allowing for a mobile phase to enter the hollow channel via the first end and exit via the second end.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

This invention discloses a design a capillary column for use with electrochemically modulated liquid chromatography (EMLC). The capillary design, which results in a marked reduction in the flow of current through the column, enables the use of a two-electrode column construction that overcomes the mechanical and electrical shortfalls of the conventional standard bore design.