Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to fragment one or more nucleic acid primers labeled with a first tag and monitor for an intensity of the first tag in a mass spectrometry (MS) method. An ion source provides a beam of ions from a polymerase chain reaction amplified sample that includes one or more nucleic acid primers labeled with the first tag. The first tag binds to one or more nucleic acid primers of a known microbe and is cleaved from the nucleic acid primers during the MS method. The mass spectrometer receives the beam of ions and is adapted to perform the MS method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample.

The present disclosure relates to a method for determining a carry over of an analyte from a previous sample into a sample of interest on a liquid chromatography mass spectrometer (LC-MS) device, the method comprising the following steps: (a) determining at least one chromatogram of said sample of interest on said LC-MS device; (b) determining a background height of the chromatogram; and (c) determining the carry over of the analyte from said previous sample into the sample of interest based on the background height. The present disclosure also relates to methods, systems, and computer program products related to the aforesaid method.

A chromatograph mass spectrometer includes a measurement unit including a mass spectrometry unit capable of MS.sup.n analysis, and to separate sample components and repeatedly perform mass spectrometry, a chromatogram display processing unit to create a chromatogram at a specific m/z and display it, a time designation unit to designate a retention time according to a user operation, a spectrum display processing unit to create an MS spectrum corresponding to the retention time and an MS.sup.n spectrum, as an MS.sup.n analysis result corresponding to the retention time, targeting an m/z of a peak in the MS spectrum or an m/z range including the m/z, and display the MS spectrum and the MS.sup.n spectrum on the same screen, the time designation section designating a retention time by a user moving a pointer, and the spectrum display processing unit, updating the display corresponding to the retention time corresponding to the pointer position.

A chromatograph mass spectrometer includes a measurement unit including a mass spectrometry unit capable of MS.sup.n analysis, and to separate sample components and repeatedly perform mass spectrometry, a chromatogram display processing unit to create a chromatogram at a specific m/z and display it, a time designation unit to designate a retention time according to a user operation, a spectrum display processing unit to create an MS spectrum corresponding to the retention time and an MS.sup.n spectrum, as an MS.sup.n analysis result corresponding to the retention time, targeting an m/z of a peak in the MS spectrum or an m/z range including the m/z, and display the MS spectrum and the MS.sup.n spectrum on the same screen, the time designation section designating a retention time by a user moving a pointer, and the spectrum display processing unit, updating the display corresponding to the retention time corresponding to the pointer position.

A gas chromatograph is provided with: a sample gas generator; a separation column; a detector; a plurality of gas supply sources; a switching unit; a regulator-; and an out-of-gas determination unit. After the out of gas determination unit has determined that the out of gas has occurred in the gas supply source supplying the carrier gas to the sample gas generator, it is configured to perform a column protection operation for changing the gas supply source fluidly connected to the sample gas generator by the switching unit.

A method of ionising a sample is disclosed comprising nebulising a sample which includes monoclonal antibody (“mAb”) molecules. A stream of monoclonal antibody droplets or charged droplets is directed so as to impact upon a target or electrode so as to form intact parent monoclonal antibody ions, intact minus light chain parent monoclonal antibody ions or light chain (“LC”) fragment monoclonal antibody ions.

An analysis device having a liquid chromatograph prevents a plurality of streams from being unusable at the same time Whether a usable stream is 0 or not among streams 1, 2, and 3 is determined. In a case where none of the streams is usable, the control unit skips a sample introduction in the cycle; and where there is one usable stream in the cycle, the stream is used. In a case where there are multiple streams that are usable in the cycle, and there are multiple streams of which the remaining number of uses of a separation column is the minimum, the stream having the smallest stream number is used; and where there is one stream of which the remaining number of uses of a separation column is the minimum, the stream to which the separation column having the minimum remaining number of uses is connected and used.

A method for the identification and localization of small molecule species in a histologic thin tissue section comprises the steps of: a) acquiring a mass/mobility image of the tissue section and generating a mass/mobility map of the small molecule species of interest for each pixel of the image; b) providing a second sample of the same tissue and extracting the small molecules of interest, separating them, and acquiring mass and ion mobility spectra from the separated small molecules; c) identifying the small molecules of interest using corresponding reference databases; and d) assigning identified small molecules to entries in the mass/mobility maps of the first tissue section by comparison of ion masses and mobilities of the identified species to those of the second thin tissue section.

A microwave microstrip resonator apparatus including a housing; a resonator within the housing; an output conductor within the housing and spaced apart from the resonator so as to define a capacitive gap therebetween; a reaction vessel configured to reside with the capacitive gap; and a power supply coupled to the resonator whereby contents within the reaction vessel are heated when energy is supplied to the resonator by the power supply. A mass spectrometer may also be coupled to an outlet end of the reaction vessel such that the contents within the reaction vessel are, simultaneously, delivered to the mass spectrometer for analysis.

A gas analyzer and a method for performing mass spectrometry analysis includes a membrane configured to receive an input flow of carrier gas. The membrane defines a variable thickness region between first and second positions along an input face of the membrane and separates the analyte sample into an output flow of analyte molecules. A mass spectrometer is disposed downstream of the membrane and includes an input orifice for receiving the output flow. The mass spectrometer is configured to perform a response profile analysis of the analyte molecules in the sample analyte.