The present disclosure relates generally to the field of analyzing a nucleic acid, such as RNA, in particular to the determination of at least one parameter of a sample composition comprising a nucleic acid, especially RNA, and optionally particles.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

A tube preparation step of preparing a resin tube that has a tube wall impregnable with a solution including a fine substance and is made of a light-transmitting resin material, a solution preparation step of preparing a solution that includes a fine fluorescent substance that emits fluorescence or a fine scattering substance that scatters light as an oscillation material and an impregnation step of causing the resin tube to be immersed in the solution and causing the tube wall of the resin tube to be impregnated with the oscillation material, are included.

A method and system for determining the dissociation constant (K.sub.d) of a reversible binding pair of a first compound and a second compound. The method comprises: injecting a sample into a capillary tube via one or more valves, wherein the sample comprises the first compound, the second compound, and a first compound-second compound complex; injecting a mobile phase into the capillary tube via said one or more valves, the sample flowing through the capillary tube under laminar flow conditions, wherein the second compound and the first compound-second compound complex is separated from the first compound by transverse diffusion; measuring time dependence of a signal that is proportional to the concentration of the first compound, both unbound and bound to the second compound using a measurement component; and determining the equilibrium dissociation constant based on the measured signal versus time dependence.

A method and system for determining the dissociation constant (K.sub.d) of a reversible binding pair of a first compound and a second compound. The method comprises: injecting a sample into a capillary tube via one or more valves, wherein the sample comprises the first compound, the second compound, and a first compound-second compound complex; injecting a mobile phase into the capillary tube via said one or more valves, the sample flowing through the capillary tube under laminar flow conditions, wherein the second compound and the first compound-second compound complex is separated from the first compound by transverse diffusion; measuring time dependence of a signal that is proportional to the concentration of the first compound, both unbound and bound to the second compound using a measurement component; and determining the equilibrium dissociation constant based on the measured signal versus time dependence.

The device is provided with a lamp lighting unit configured to perform an operation for lighting a deuterium lamp by controlling a lamp drive unit and a heater drive unit, an abnormality determination unit configured to determine, in a prescribed sequence, whether or not a voltage or a current detected by a lamp voltage detection unit, a lamp current detection unit, a heater voltage detection unit, and a heater current detection unit is abnormal, and an abnormality cause identification unit configured to identify, in a case the voltage or the current is determined to be abnormal by the abnormality determination, the cause of the abnormality, based on the timing at which the abnormality determination unit determines that the voltage or the current is abnormal.

The device is provided with a lamp lighting unit configured to perform an operation for lighting a deuterium lamp by controlling a lamp drive unit and a heater drive unit, an abnormality determination unit configured to determine, in a prescribed sequence, whether or not a voltage or a current detected by a lamp voltage detection unit, a lamp current detection unit, a heater voltage detection unit, and a heater current detection unit is abnormal, and an abnormality cause identification unit configured to identify, in a case the voltage or the current is determined to be abnormal by the abnormality determination, the cause of the abnormality, based on the timing at which the abnormality determination unit determines that the voltage or the current is abnormal.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.