A fraction collector control device includes a peak designation part that displays a chromatogram for setting and requires a user to select at least one peak in the chromatogram for setting, a parameter extraction part that extracts, as a parameter, a signal level and/or a slope at a start point and an end point of each peak designated by a user, and a threshold candidate generation part that generates, based on the parameter extracted by the parameter extraction part, a threshold candidate usable as a common threshold for detecting start points of all peaks designated by a user and a common threshold for detecting end points of all peaks designated by a user.

A preparative liquid chromatograph includes a liquid chromatograph section, a trap section, an eluent supply section, a collector, and a flow path switching section. The flow path switching section is configured to be selectively switched to a component trap mode that connects the liquid chromatograph section and the trap section in such a way that a sample component separated in a separation column is trapped by a trap column of the trap section; and a collection mode that connects the eluent supply section and the trap section and connects the trap section and the collector in such a way that the components trapped in the trap column are eluted by an eluent from the eluent supply section and are guided to the collector.

A preparative liquid chromatograph includes a liquid chromatograph section, a trap section, an eluent supply section, a collector, and a flow path switching section. The flow path switching section is configured to be selectively switched to a component trap mode that connects the liquid chromatograph section and the trap section in such a way that a sample component separated in a separation column is trapped by a trap column of the trap section; and a collection mode that connects the eluent supply section and the trap section and connects the trap section and the collector in such a way that the components trapped in the trap column are eluted by an eluent from the eluent supply section and are guided to the collector.

A management system (1) for managing an operation of a preparative LC (100), the management system (1) including an injection registration part (2) configured to display an injection registration screen in response to a request from the user and register a container designated by the user on the injection registration screen as an injection target container, a result display part (6) configured to display a chromatogram created based on an output signal of a detector (108) of the preparative LC, create fractionation information indicating a correspondence relationship between a position on the chromatogram of each fraction collected in an individual container by a fractionator (112) of the preparative LC and a position of a container in which each fraction is collected, and display the created fractionation information as a fractionation result screen, a reinjection registration part (8) configured to allow the user to designate a fraction to be reinjected on the fractionation result screen, and configured to register a container in which the fraction designated by the user is collected as a reinjection target container when the fraction to be reinjected is designated by the user, and a controller (4) that controls operation of an injector of the preparative LC so that liquid contained in the container registered as the injection target container or the reinjection target container is injected.

A preparative liquid chromatograph includes a liquid feeding pump (2) that feeds a mobile phase, an injector (4) that injects a sample into the mobile phase at a downstream of the liquid feeding pump (2), a separation column (6) for separating components in the sample injected into the mobile phase by the injector (4) at a downstream of the injector (4), and an eluate fractionator (8) configured to divide a flow of the eluate from the separation column (6) into a flow of a minute flow rate and another flow at a downstream of the separation column (6) and to extract at least a part of an eluate that forms the flow of a minute flow rate into an fractionation container (22).

The present invention describes a method of operating a fractionation collector in chromatography, and a chromatograph system for carrying out the method, wherein a solvent composition is pumped into a chromatography column, which contains at least a portion of a first solvent that is liquid under normal conditions and contains a portion of a second solvent that is gaseous at normal conditions, and the composition flowing out of the chromatography column is at least partially introduced into a detector, wherein a composition containing the first solvent, after leaving the chromatography column, is introduced into a collection vessel of the fraction collector in dependence of the detector signal, and the amount of liquid that is introduced into a collecting vessel of the fraction collector is selected in dependence of the portion of said first solvent.

The present invention describes a method of operating a fractionation collector in chromatography, and a chromatograph system for carrying out the method, wherein a solvent composition is pumped into a chromatography column, which contains at least a portion of a first solvent that is liquid under normal conditions and contains a portion of a second solvent that is gaseous at normal conditions, and the composition flowing out of the chromatography column is at least partially introduced into a detector, wherein a composition containing the first solvent, after leaving the chromatography column, is introduced into a collection vessel of the fraction collector in dependence of the detector signal, and the amount of liquid that is introduced into a collecting vessel of the fraction collector is selected in dependence of the portion of said first solvent.

A preparative liquid chromatograph including an analysis channel (2), an injector (6), a separation column (8), a detector (10), a plurality of fraction collectors (14-1 to 14-n), a switching valve (12) that switches fraction collectors (14-1 to 14-n) to be connected to the outlet of the detector (10) among the plurality of fraction collectors (14-1 to 14-n), and a controller (16) for controlling operations of the plurality of fraction collectors (14-1 to 14-n) and the switching valve (12). In a case where the number of remaining unused collection containers of the fraction collector in use, which is connected to the outlet of the detector, falls below the number of buffers which is an integer of two or more, the controller (16) is configured to switch the switching valve (12) while the fraction collector in use is not performing a peak collection operation.

A preparative liquid chromatograph for separating and extracting components in a sample, the preparative liquid chromatograph including a separation channel (2) through which a mobile phase flows, an injector (6) that injects the sample into the mobile phase, a separation column (8) for separating components in the sample injected into the mobile phase, the separation column (8) being provided downstream of the injector (6) on the separation channel (2), a detector (10) for detecting peaks of the components separated by the separation column (8), a fraction collector (12) configured to collect an eluate from the separation column (8) into a plurality of collection containers (20:22) at a downstream of the detector (10) while fractionating the eluate, and a controller (14) for controlling operation of the fraction collector (12), wherein the fraction collector (12) is provided with peak collection containers (20) and waste liquid collection containers (22), each peak collection container (20) is used for collecting a peak portion including a collection target peak detected by the detector (10) in the eluate, and each waste liquid collection container (22) is used for collecting a waste liquid portion not including the collection target peak in the eluate, and the controller (14) is configured to collect the peak portion including the collection target peak detected by the detector (10) in the eluate in the peak collection container (20), and to collect at least a part of the waste liquid portion in the waste liquid collection container (22).

A preparative liquid chromatograph for separating and extracting components in a sample, the preparative liquid chromatograph including a separation channel (2) through which a mobile phase flows, an injector (6) that injects the sample into the mobile phase, a separation column (8) for separating components in the sample injected into the mobile phase, the separation column (8) being provided downstream of the injector (6) on the separation channel (2), a detector (10) for detecting peaks of the components separated by the separation column (8), a fraction collector (12) configured to collect an eluate from the separation column (8) into a plurality of collection containers (20:22) at a downstream of the detector (10) while fractionating the eluate, and a controller (14) for controlling operation of the fraction collector (12), wherein the fraction collector (12) is provided with peak collection containers (20) and waste liquid collection containers (22), each peak collection container (20) is used for collecting a peak portion including a collection target peak detected by the detector (10) in the eluate, and each waste liquid collection container (22) is used for collecting a waste liquid portion not including the collection target peak in the eluate, and the controller (14) is configured to collect the peak portion including the collection target peak detected by the detector (10) in the eluate in the peak collection container (20), and to collect at least a part of the waste liquid portion in the waste liquid collection container (22).