A system for solventless calibration of volatile or semi-volatile compounds and methods thereof. The system includes a fluid path having a first end configured to be operably coupled to a fluid source and a second end configured to be operably coupled to the analytical instrument. A solid sorbent is disposed along the fluid path and is configured to absorb an analyte. The flow of fluid along the fluid path from the first end to the second end causes absorbed analyte to be desorbed from the solid sorbent at a desired concentration to the instrument.

Systems and methods are described for reducing lab-to-lab and/or instrument-to-instrument variability of Multi-Attribute Methods (MAM) analyses via run-time signal intensity calibration. In various aspects, multiple MAM-based instruments each have detectors and different instrument conditions defined by different instrument models or sets of settings. Each MAM-based instrument receives respective samples and a reference standard as a calibrant. Each MAM-based instrument detects, via its detector, sample isoforms of its respective sample and reference standard isoforms of the reference standard. The MAM-based instruments are associated with processor(s) that determine, via respective MAM iterations, correction factors and sample abundance values corresponding to the sample isoforms. The correction factors are based on the reference standard, and the sample abundance values are based on the correction factors. A variance value of the sample abundance values may be reduced based on correction factors of each of the MAM-based instruments.

A chromatography system includes a gradient delay volume defined as an overall fluid volume between where gradient is proportioned until an inlet of a chromatography column, a pump pumping a flow of gradient; and at least one valve located downstream from the pump, the at least one valve having a plurality of ports including an inlet port that receives the flow of gradient from the pump and an outlet port through which the flow of gradient exits the at least one valve, the at least one valve having at least two positions. A first position of the at least two positions of the at least one valve increases the gradient delay volume of the chromatography system relative to when the at least one valve is in a second position.

The device is provided with a lamp lighting unit configured to perform an operation for lighting a deuterium lamp by controlling a lamp drive unit and a heater drive unit, an abnormality determination unit configured to determine, in a prescribed sequence, whether or not a voltage or a current detected by a lamp voltage detection unit, a lamp current detection unit, a heater voltage detection unit, and a heater current detection unit is abnormal, and an abnormality cause identification unit configured to identify, in a case the voltage or the current is determined to be abnormal by the abnormality determination, the cause of the abnormality, based on the timing at which the abnormality determination unit determines that the voltage or the current is abnormal.

A method of operating a chromatography column is described for use in methods of manufacture for producing anti-IL-12/IL-23p40 antibodies, e.g., the anti-IL-12/IL-23p40 antibody STELARA® (ustekinumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value.

A method of detecting gas includes determining and storing, by a controller, a zero level of a photoionization detector using ambient air inflow when an ultraviolet lamp is in a turned OFF state, wherein the stored zero level is based on an ambient temperature; sampling, by the controller, an output of a detector electrode of the photoionization detector when the ultraviolet lamp is in a turned ON state; and comparing the sampled output of the detector electrode to the stored zero level to determine if a threshold concentration of a gas is present.

A method for detecting and mitigating compounds in an aquaculture system. The method for detecting and mitigating off-flavorings includes, taking at least one sample of water from at least one portion of a prospective aquaculture system that contains aquaculture, testing the at least one sample to determine at least one concentration of at least one off-flavor compound therein, and utilizing the data obtained from testing so as to regulate at least one content contained within the aquaculture system.

The present disclosure provides methods of identifying or certifying an animal that consumed a traceable diet comprising a C.sub.1 metabolizing microorganism.

The present invention provides reagents for instrumentation quality control and methods of use thereof. In particular, sets of peptides or other molecules are provided for evaluating the performance of instruments with mass spectrometry (MS) and/or liquid chromatography (LC) functionalities.

An absorbance detector includes a sample cell, a light source for irradiating the sample cell, a photo sensor, an optical system for guiding light emitted from the light source to the sample cell and guiding light that has been transmitted through the sample cell to the photo sensor, a reference signal acquirer configured to acquire a detection signal of the photo sensor when the sample solution is not flowing through the sample cell as a reference signal for each analysis of the sample, a calculator configured to find absorbance of the sample based on a measurement signal obtained by the photo sensor in the analysis and the reference signal acquired for the analysis when an analysis of the sample is carried out, and an analysis data storage configured to associate data of the absorbance found by the calculator and data of the reference signal to each other for storage.