A liquid refining apparatus is disclosed. The liquid refining apparatus includes a substrate, a loader which is formed on the substrate and configured to receive a first liquid, a filter which is configured to reduce a concentration of at least one substance contained in the first liquid to obtain a second liquid with a reduced concentration of the at least one substance, a reactor which is configured to mix the second liquid with a reactant for target substance detection to obtain a third liquid containing, among a plurality of substances contained in the second liquid, a first substance which undergoes a predetermined reaction with the reactant and a second substance which does not undergo the predetermined reaction with the reactant, and a separator which is configured to separate the first substance and the second substance.

An optical metrology device characterizes a test object using a phase shift interferometer with synchronous time varying optical frequency shifts. A light source generates a beam having a time varying frequency, which is divided into two collinear, orthogonally polarized beams that differ by a first frequency shift. One or more optical cavities receive the beams and produce a pair of reference beams that differ from each other in frequency by the first frequency shift and a pair of test beams with a second frequency shift induced by the one or more optical cavities. The test beams differ from each other by the first frequency shift and differ from the reference beams by the second frequency shift. The first frequency shift has a pre-defined relationship with respect to the second frequency shift to generate interference between a reference beam and test beam that have frequency shift magnitudes with the pre-defined relationship.

An NIR analyzer with the optical probes across a pipe, or in a bypass configuration, after a stabilizer in an oil or condensate production plant. Prior to use, liquid samples from the plant are analyzed in a chemical lab to obtain reference vapor pressure or compositional values. A chemometric model using known techniques is then built with the captured absorption spectra and the reference lab results. Preprocessing methodologies can be used to help mitigate interferences of the fluid, instrument drift, and contaminate build up on the lenses in contact with the fluid. The chemometric model is implemented through the NIR analyzer as the calibration curve to predict the vapor pressure or other values of the flowing fluid in real time.

A handheld LIBS device and method includes a laser assembly producing two pulsed single spatial mode output beams and a focusing optic which combines the two pulsed single spatial mode output beams at a focal point at a sample. The laser assembly includes a laser assembly housing with an output coupler window for the two pulsed single spatial mode output beams, a gain medium in the laser assembly housing between the output coupler window and an adjustable prism mount in the laser assembly housing holding a prism configured to establish two light paths through the gain medium, a source in the laser assembly housing providing pump energy to the gain medium, and a Q-switch positioned between the prism and the gain medium.

A method of foreign object debris discrimination includes illuminating a particle located within a sensing volume with a modulated electromagnetic radiation pulse emitted from a source; receiving one or more electromagnetic radiation return signals that have been scattered by the particle illuminated by the modulated electromagnetic radiation pulse at a detector; mixing, using a controller, the electromagnetic radiation return signal of amplitude I.sub.RS and frequency f.sub.RS with a reference signal of amplitude I.sub.LS and frequency f.sub.RS; analyzing, using the controller, an amplitude of the mixed signal √{square root over (I.sub.LSI.sub.RS)}, and frequency of the mixed signal, f.sub.RS−f.sub.LS; and classifying, using the controller, a particle position, a velocity, and electromagnetic characteristic of the particle based on the amplitude, √{square root over (I.sub.LSI.sub.RS)}, and frequency, f.sub.RS−f.sub.LS of the mixed signal.

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of-ordinary skill in the art in which this invention belongs.

The invention encompasses analyzers and analyzer systems that include a single molecule analyzer, methods of using the analyzer and analyzer systems to analyze samples, either for single molecules or for molecular complexes. The single molecule uses electromagnetic radiation that is translated through the sample to detect the presence or absence of a single molecule. The single molecule analyzer provided herein is useful for diagnostics because the analyzer detects single molecules with zero carryover between samples.

This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.

A method for detecting the quality of cell culture fluid based on Raman spectral measurement. The method comprises the following steps: collecting cell culture fluid; collecting, processing and analyzing a Raman spectral signal; measuring an original Raman spectral signal of a metabolite in the cell culture fluid using a Raman spectra technique; determining whether the original Raman spectral signal is qualified, and carrying out data signal processing on the qualified original Raman spectral signal to obtain analyzable signals; and then carrying out difference statistical analysis on the analyzable signals to obtain difference signals; carrying out modeling using the difference signals; classifying the difference signals using a support vector machine; and distinguishing the spectral signals of normal and abnormal cell culture fluid to obtain a quality result of the cell culture fluid. Difference signals in cell culture fluid are detected by means of Raman spectra to detect the quality of the cell culture fluid, thereby achieving the purpose of non-invasive evaluation of a cell growth state; and the method is convenient, effective and low-cost, and can achieve large-scale industrialization and streamlining.

A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.