A method of directly measuring moisture content in an oil-filled transformer includes using an optical fiber that having a grating sensor, such as a Fiber Bragg grating, defined in the optical fiber. The various conductors (windings) in the transformer are insulated using an insulator such as paper and insulating oil is filled inside the transformer. Moisture in the transformer is absorbed by the paper that surrounds the windings. A moisture content at a specific location can be measured by placing the optical fiber with the grating sensor directly at the specific location to be measured. A physical parameter of the paper that absorbed moisture changes over time, causing a change in the grating sensor of the optical fiber which changes the spectral response of optical signals that are reflected by the grating sensor. The method provides an accurate method of measuring the moisture inside the transformer at the specific location.

An instrument is provided that can monitor nucleic acid sequence amplification reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path.

A structured illumination microscope includes: a first illumination optical system configured to irradiate, from a first direction, a sample with activating light for activating a fluorescent substance included in the sample; a second illumination optical system configured to irradiate, from a second direction that is different from the first direction, the sample with interference fringes of exciting light for exciting the fluorescent substance; a control unit configured to control a direction and a phase of the interference fringes; an imaging optical system configured to form an image of the sample irradiated with the interference fringes; an imaging element configured to take the image formed by the imaging optical system to generate a first image; and a demodulation unit configured to generate a second image by using a plurality of the first images generated by the imaging element.

Apparatus and method for performing depth sectioned fluorescence imaging of a turbid sample including a fluorescent turbid medium, uses an apparatus for quantitative modulated fluorescence imaging, the apparatus including projection optics with a first optical axis, to expose the turbid sample to a periodic pattern of excitation radiation to provide depth-resolved discrimination of fluorescent structures within the turbid medium; an image capture module, including a second optical axis and a detection beam path, to receive a data image from the sample; and a signal processor to transform the data image from the sample, spatially filter the transformed data image from the sample, and reconstruct the filtered, transformed data image from the sample.

Devices and methods for recording dynamics of cellular and/or biochemical processes, including a device including one or more dispersive elements configured to receive a pulsed laser beam with a spectrum of different wavelengths and disperse the spectrum of the pulsed laser beam; and one or more first elements configured to receive the dispersed spectrum of the pulsed laser beam, and generate a multiphoton excitation area in a biological sample by re-overlapping in time and space the dispersed spectrum of the pulsed laser beam on an area in the biological sample, wherein the device is configured to record at high speed changes of cellular and biochemical processes of a population of cells of the biological sample based on generation of the multiphoton excitation area in the biological sample.

A gas detector (10) includes a cell internal space (130) into which a target gas is supplied, the target gas exhibiting an absorption peak in an absorption spectrum; a light source (410) configured to generate light having at least a wavelength belonging to the absorption peak; and a photodetector (420) configured to detect the light that has emitted from the light source (410) and has propagated through the cell internal space (130). The gas detector (10) further includes a conductive thin film (220) in which a plurality of optical apertures (222) are regularly arranged such that a transmission peak in a transmission spectrum is superimposed over the absorption peak in the absorption spectrum along a wavelength axis. The conductive thin film (220) is provided on an optical path extending from the light source (410) to the photodetector (420), and is provided so as to be contactable with the target gas within the cell internal space (130).

Systems and methods are disclosed relating to composite photonic materials used to design structures and detecting material deformation for the purpose of monitoring structural health of physical structures. According to one aspect, a composite structure is provided that includes a base material, an optical diffraction grating and one or more fluorophore materials constructed such that localized perturbations create a measurable change in the structure's diffraction pattern. An inspection device is also provided that is configured to detect perturbations in the composite structure. The inspection device is configured to emit an inspecting radiation into the structure and capture the refracted radiation and measure the change in the diffraction pattern and quantify the perturbation based on the wavelength and the angular information for the diffracted radiation.

A spectrophotometer 300 includes a white light source 212, condenser lenses 242a, 242b that collect light emitted from the white light source 212, a slit 245 that diffracts the light collected by the condenser lenses 242a, 242b, a concave diffraction grating 246 that splits the light having passed through the slit 245, and a multi-wavelength detector 248 having a plurality of photodetection elements 304 that detect the light split by the concave diffraction grating 246, and each of the plurality of photodetection elements 304 included in the multi-wavelength detector 248 is arranged at an image position of the concave diffraction grating 246.

An optical component for illuminating a sample region with a periodic light pattern comprises: a first waveguide, a further waveguide and an optical splitter. The optical splitter has an input for receiving light, a first output and a second output. The first waveguide is optically coupled to the first output, to direct the first input light into the sample region in a first direction. The second output is optically coupled to the sample region to direct second input light into the sample region in a second direction. The further waveguide is arranged to receive third input light which is directed into the sample region in a third direction. The first direction, second direction and third direction are different from one another. The first and second input light interferes to form a periodic pattern in the sample region. The optical component may be used for structured illumination microscopy.

In an inspection apparatus, a target on the surface is illuminated with illuminating radiation that comprises first and second illuminating components. The illuminating components form one or more periodic illuminating patterns on the surface. A plurality of scattered radiation patterns formed by the illuminating radiation after scattering by the target is captured at a detector for a number of values of a controllable characteristic of at least one of the illuminating components. The captured radiation is then used to reconstruct data describing the target.