Provided herein is an apparatus, including an optical characterization device; a photon detector array configured to sequentially receive a first set of photons scattered from surface features of an article and a second set of photons scattered from surface features of the article and subsequently processed by the optical characterization device; and a chemical characterization means for chemically characterizing the surface features of the article, wherein the chemical characterization means is configured for processing the first set of photons received by the photon detector array and the second set of photons received by the photon detector array.

Apparatus and methods for acquiring a Raman spectral map of a sample including a material species. The apparatus includes: a pulsed illumination source providing pulsed illumination radiation for exciting the sample and producing scattered radiation; a microscope objective focusing the pulsed illumination radiation onto a region of the sample corresponding to a data point of the map, and collecting emitted radiation from the region; a translation stage translating the sample relative to the microscope objective in at least two directions; a spectral filter spectrally filtering the emitted radiation collected by the objective to obtain a filtered portion of radiation corresponding to a characteristic Raman spectral feature of the material species; a detector receiving the filtered portion and providing output electrical pulses indicative thereof; and readout electronics applying a time gate to the output electrical pulses to distinguish detection events corresponding to the Raman scattered radiation from events associated with photoluminescence.

A method and an apparatus for analyzing a component of an object are provided. The apparatus includes an image sensor including an optical module, and the optical module includes a light source configured to emit a source light, a first detector configured to detect a first light that is scattered or reflected from the object on which the emitted source light is incident, and a second detector configured to detect a second light that is emitted by the light source but is not incident on the object. The apparatus further includes a processor configured to calculate a scattering coefficient and an absorption coefficient, based on the detected first light and the detected second light, and analyze the component of the object, based on the calculated scattering coefficient and the calculated absorption coefficient.

Systems, methods, and techniques for optofluidic analyte detection and analysis using multi-mode interference (MMI) waveguides are disclosed herein. In some embodiments, spatially and spectrally multiplexed optical detection of particles is implemented on an optofluidic platform comprising multiple analyte channels intersecting a single MMI waveguide. In some embodiments, multi-stage photonic structures including a first stage MMI waveguide for demultiplexing optical signals by spatially separating different wavelengths of light from one another may be implemented. In some embodiments, a second stage may use single-mode waveguides and/or MMI waveguides to create multi-spot patterns using the demultiplexed, spatially separated light output from the first stage. In some embodiments, liquid-core MMI (LC-MMI) waveguides that are tunable by replacing a liquid core, heating/cooling the liquid core, and/or deforming the LC-MMI to change its width may be implemented in one or more of the analyte detection/analysis systems disclosed herein.

A multiwavelength-light-radiating apparatus (1) includes: a light source (11) that radiates continuous light (Lc); a diffracting part (12) that diffracts the continuous light (Lc) into numerous monochromatic lights (Lm), whose wavelengths differ from one another, and emits the numerous monochromatic lights (Lm); numerous optical waveguides (2) that respectively transmit the numerous monochromatic lights (Lm) emitted from the diffracting part (12) from incident ends (21) to output ends (22) where the numerous monochromatic lights (Lm) are respectively emitted; and a sample-placement part (3) that holds numerous samples such that the output ends (22) of the numerous optical waveguides (2) respectively oppose the samples. The numerous monochromatic irradiation lights, whose wavelengths differ from one another, are arranged to be radiated simultaneously onto the numerous samples, one light per sample.

An optical assembly for the illumination and hyperspectral evaluation of an object, having a light source or an optical element at which a light source radiates, wherein the light source or the optical element is designed to divide pairs of unambiguously assignable photons into a first light beam and a second light beam so that the first light beam hits a first detector system and the second light beam is directed at an object and light radiation coming from the object is directed at an optical element which spectrally decomposes light radiation and, from the optical element spectrally decomposing said light radiation, is directed at a second detector system. The first light beam can also be directed at a spectrally decomposing optical element and, from there, at a first detector system, and the light radiation coming from the object can be directed directly at the second detector system. The first detector system is designed to perform a spatially resolved sensing of the first light beam, and the first detector system or the second detector system is designed to perform a spectrally resolved sensing of the second light beam. The detector systems are connected to an electronic evaluation unit, by means of which the measurement signals captured with spatial and spectral resolution are associated. The first and second light beams are spectrally, spatially and temporally correlated.

An optoacoustic sensor includes a liquid crystal (LC) cell formed between top and bottom plates of transparent material. A transverse grating formed across the LC cell that forms an optical transmission bandgap. A CL is aligned to form a spring-like, tunable Bragg grating that is naturally responsive to external agitations providing a spectral transition regime, or edge, in the optical transmission bandgap of the transverse grating that respond to broadband acoustic waves. The optoacoustic sensor includes a narrowband light source that is oriented to transmit light through the top plate, the LC cell, and the bottom plate. The optoacoustic sensor includes an optoacoustic spectrometer that is oriented below the bottom plate to receive the transmitted light and to record a time-domain modulation of transmission intensity at a selected one of a falling and rising edge of the transmission bandgap for detecting analog acoustic vibration.

A light modulation device and a single-channel spectrum detection system are provided. The light modulation device includes: a light guide plate; a dispersing component configured to disperse received light into light of different wavelengths and to diffract the light of different wavelengths into the light guide plate at different angles; and a dynamic filtering component configured to prevent light of a selected wavelength in the light guide plate from entering the dynamic filtering component such that the light of the selected wavelength emits out from the light guide plate, and to make light of non-selected wavelengths in the light guide plate enter the dynamic filtering component such that the light of the non-selected wavelengths is filtered out from the light guide plate.

A microfluidic apparatus is provided. The microfluidic apparatus includes a first substrate; a microfluidic layer on the first substrate and defining a microfluidic channel, wherein the first substrate having a first side closer to the microfluidic layer, and a second side away from the microfluidic layer, the first side and the second side opposite each other; a plurality of detectors on a side of the microfluidic channel away from the first substrate; a unitary grating plate on the second side of the first substrate and including a plurality of grating blocks of different wavelength selectivity; and a light extraction layer including a plurality of light extractors on the first side of the first substrate and configured to extract light diffracted by the plurality of grating blocks out of the first substrate.

The technology disclosed relates to structured illumination microscopy (SIM). In particular, the technology disclosed relates to capturing and processing, in real time, numerous image tiles across a large image plane, dividing them into subtiles, efficiently processing the subtiles, and producing enhanced resolution images from the subtiles. The enhanced resolution images can be combined into enhanced images and can be used in subsequent analysis steps. The technology disclosed includes logic to reduce computing resources required to produce an enhanced resolution image from structured illumination of a target. A method is described for producing an enhanced resolution image from images of a target captured under structured illumination. This method applies one or more transformations to non-redundant data and then recovers redundant data from the non-redundant data after the transformations.