Methods and systems are provided for synchronizing image capture at a multi-detector imaging system. In one example, a method includes coordinating cycling of each microscope assembly of the multi-detector imaging system through a selection of illumination channels, each microscope assembly configured to obtain an image of a portion of one of more than one microplate wells simultaneously, to generate complete images of the more than one microplate wells concurrently.

Embodiments disclose a device for testing biological specimen. The device includes a sample carrier and a detachable cover. The sample carrier includes a specimen holding area. The detachable cover is placed on top of the specimen holding area. The detachable cover includes a magnifying component configured to align with the specimen holding area. The focal length of the magnifying component is from 0.1 mm to 8.5 mm. The magnifying component has a linear magnification ratio of at least 1.

Provided is a microscope system including: an optical fiber in which laser light emitted from a light-source apparatus propagates; a microscope that irradiates a specimen with the laser light propagated in the optical fiber and that obtains an image of the specimen; a mode-scrambling device portion that causes elastic waves to propagate in the optical fiber to form elastic wave interference fringes in the optical fiber; and a control device that controls the driving of the mode-scrambling device.

A Brillouin modality can be supplemented by an auxiliary modality, such as an optical imaging modality or a spectroscopy modality. In some embodiments, the auxiliary modality can be used to guide the Brillouin measurement to a desired region of interest, so that acquisition times for the Brillouin measurement can be reduced as compared to interrogating the entire sample. The auxiliary modality may have an acquisition speed faster than that of the Brillouin modality. In some embodiment, the auxiliary modality determines a composition of materials within a voxel in the sample interrogated by the Brillouin modality. Using the information provided by the auxiliary modality, the Brillouin signatures corresponding to the materials within the voxel can be unmixed, thereby providing a more accurate measurement of the sample.

The invention relates to a method for punctiform illumination of a sample (1) in a microscope, more particularly a MINFLUX microscope, using illumination light, with the sample (1) being sequentially illuminated at the illumination points (3) of a predefined or predefinable illumination point pattern (2). The method is distinguished in that a lateral extent of the illumination point pattern (2) is smaller than the longest wavelength of the illumination light and in that the illumination points (3) are always illuminated exclusively with a time offset and in that a distinct individual light source (4) of a plurality thereof is assigned to each illumination point (3) of the 10 illumination point pattern (2) and each illumination point (3) is illuminated by the focus of an illumination light bundle (5) of the individual light source (4) assigned thereto.

The present invention relates to digital pathology. In order provide enhanced use of available imaging radiation, a digital pathology scanner (10) is provided that comprises a radiation arrangement (12), a sample receiving device (14), an optics arrangement (16), and a sensor unit (18). The radiation arrangement comprises a source (20) that provides electromagnetic radiation (22) for radiating a sample received by the sample receiving device. Further, the optics arrangement comprises at least one of the group of a lens (24) and a filter (26) that are arranged between the sample receiving device and the sensor unit. The sensor unit is configured to provide image data of the radiated sample. Still further, a lens array arrangement (28) is provided that comprises at least one lens array (30) arranged between the source and the sample receiving device. The at least one lens array comprises a plurality of linear cylindrical lenses (32) that modulate the electromagnetic radiation from the source such that, in an object plane, a radiation distribution pattern (34) is generated with a plurality of first parts of intensified radiation and a plurality of second parts of weak radiation.

The phase modulation device 3 includes a first phase modulation element 11 which modulates a phase of a light flux in accordance with a voltage applied to each of a plurality of first electrodes in accordance with a first ratio of a second aberration component to a first aberration component of a wave front aberration generated by an optical system including an objective lens 4; a second phase modulation element 12 which modulates a phase of a light flux in accordance with a voltage applied to each of a plurality of second electrodes in accordance with a second ratio of the second aberration component to the first aberration component; and a control circuit 13 which controls voltages applied to each of first electrodes and each of second electrodes in accordance with a distance from the objective lens to a light focusing position of the light flux.

A microscope (10) for detecting images of an object (14) located in an object plane (12) is described, comprising a microscope stand (18); a microscope objective (20); a light source (22) integrated into the microscope stand (18); and a beam splitter (24), integrated into the microscope objective (20), for coupling in a coaxial incident illumination.

A light sheet microscope includes a sample chamber in which a cover slip or slide is arrangeable, which has a surface that defines a partially reflective interface and which has a further surface that defines a further partially reflective interface. The two interfaces are arranged at different distances from an objective. The light sheet microscope further includes an optical system having the objective facing toward the cover slip or slide, an illumination apparatus, which is designed to generate a light sheet, a sensor, and a processor. The two interfaces are formed in that two optical media are applicable in the sample chamber. The light sheet microscope forms a measuring device for acquiring a measured variable. The sensor is designed to acquire the intensities and/or the incidence locations of the two reflection light beams.

The invention provides for a system and methods of directly delivering excitation light to a sample by a path that avoids traveling though the objective and dichroic mirror. Certain embodiments of the system may include an excitation light source, and a series of mirrors and lenses to direct the excitation light to a diffuser unit. The diffuser unit may then generate a configurable illumination profile to evenly illuminate a sample.