An optical apparatus having an illumination module with a carrier, which has at least one light-transmissive region, for example. The illumination module has a plurality of light sources, which are arranged on the carrier.

A fully automatic microscopic scanner, comprising an image acquisition mechanism (IAM) with a line array camera. The IAM and the microscopic scanning mechanism move along X/Y/Z axis under the action of a power control mechanism. The light source mechanism illuminates an area greater than or equal to the total area of the tray and has a flat light source with a condenser, a cooling fan, a water cooling unit. A general control mechanism and a laser pre-focus system connects to the light source mechanism. The fully automatic microscopic scanner links the microscopic scanning mechanism and the IAM with the light source mechanism. Flexible adjustment of lens is achieved by making the lens move forwards and backwards, leftwards and rightwards and up and down. The light source mechanism and laser pre-focus system, the stage and the light source are fixed during the scanning process to achieve real-time accurate and fast focusing.

An optical apparatus comprises an illumination module (100) comprising a carrier (110), which has at least one light-transmissive region (112), for example. The illumination module (100) comprises a plurality of light sources (111), which are arranged on the carrier (110).

The invention relates to EPI lighting which allows transmitted light-bright field- or transmitted light-dark field-imaging or phase contrast imaging of a microscopic sample. For this purpose, a flat reflector is used which is located opposite the observer side and which brings about a deflection of the illumination beam of light. The flat reflector has a plane normal and an effective perpendicular which differs from the plane normal, or it is in the form of a retroreflector.

The present disclosure is directed to an optical measurement unit for a scanning device, to a scanning device and a method for operating a scanning device, for high throughput sample analysis of biological samples, wherein an illumination system is used to emit light of at least two different illumination wavelength ranges, and wherein an imaging system is used to detect light of at least two different detection wavelength ranges, in order to detect electromagnetic radiation within a field of view for determining the positioning of a sample within the field of view.

A microscope and a microscopy method for imaging an object, wherein use is made of a microscope comprising an objective which defines an optical axis and a focal plane perpendicular thereto, and an adjustable correction optical unit which, at the objective, corrects a spherical aberration occurring when imaging the object with a certain depth position of the focal plane. The method comprises the following steps: determining an actual type of object; reading a database in which refractive indices of different types of objects are stored in order to determine the refractive index of the object; using a relationship between the refractive index and the spherical aberration caused by the object in order to ascertain an adjustment value of the correction optical unit such that there is a reduction in the spherical aberration in the focal plane; adjusting the correction optical unit to the adjustment value; and imaging the object.

A sample observation apparatus includes a light source unit, an illumination optical system, an observation optical system, a light detection element, a scanning unit, a holding member, and an image processing device. A light spot is formed by the illumination optical system. The scanning unit moves the light spot and the holding member relative to each other. A pupil of the observation optical system and the light detection element are positioned at a position conjugate to a pupil position of the illumination optical system. The image processing device generates an image of a sample based on a predetermined image and a filter, and the predetermined image is an image based on a signal output from the light detection element. The filter includes a first region and a second region, and a value in the first region is greater than a value in the second region.

A portable biological microscopic image analysis system is suitable for analyzing a sample on a slide in real time, and the sample comes from an animal. The portable biological microscopic image analysis system includes a handheld electronic device and a microscope kit; the handheld electronic device includes an image capture unit, an image analysis module electrically connected to the image capture unit, and a real-time state analysis module electrically connected to the image analysis module; the microscope kit is detachably mounted on the handheld electronic device; wherein the image capture unit is used to obtain an image related to the sample through the microscope kit, the image analysis module is used to obtain cell information corresponding to the animal according to the image, and the real-time state analysis module is used to obtain state information corresponding to the animal according to the cell information.

Disclosed herein is a smartphone microscope hardware for facilitating diagnosing microscopic objects in a sample of an object, in accordance with some embodiments. Accordingly, the smartphone microscope hardware may include a smartphone case, a magnifier, a glass slide, and a light source. Further, the smartphone case is configured to interface with a smartphone. Further, the magnifier is attached to the smartphone case. Further, the glass slide is configured for receiving the sample. Further, the camera is configurable for capturing an image of the sample. Further, the magnifier is configured for magnifying the image prior to the capturing. Further, a processing device of the smartphone is configurable for analyzing the image, identifying a microscopic object, and generating a notification. Further, a display device of the smartphone is configurable for displaying the image and the notification. Further, the light source is disposed adjacent to a second side of the glass slide.

A microscope for computational microscopic layer separation may include an imaging device that includes a lens and an image sensor, an illumination system for illuminating a sample, and an actuator to adjust an axial position of a focal plane with respect to the sample. The microscope may also include a processor operatively coupled to the imaging device and the illumination system. The processor may be configured to measure, using the image sensor and the illumination system, optical aberrations of the imaging device at the axial position, and determine whether to adjust the focal plane with respect to the sample in response to the one or more optical aberrations. Various other systems and methods are also disclosed.