A digital holographic microscope in which two digital holographic microscopes for detecting a fluorescence image and a phase image, respectively, are combined to be able to three-dimensionally measure a fluorescence image and a phase image at the same time, and perform measurement at a high SN ratio in all the polarization states including random light polarization. A first holographic optical system that, by using laser light, acquires a phase three-dimensional image due to interference light generated by superimposing object light which passes through a sample stage and reference light which does not pass through the sample stage onto each other. A second holographic optical system that, by using fluorescent excitation light, acquires a fluorescence three-dimensional image due to a fluorescence signal light, wherein phase measurement by the first holographic optical system and fluorescence measurement by the second holographic optical system are performed at the same time.

The present disclosure relates to devices and methods configured to perform drug screening on cells. At least one embodiment relates to a lens-free device for performing drug screening on cells. The lens-free device includes a substrate having a surface. The lens-free device also includes a light source positioned to illuminate the cells, when present, on the substrate surface with a light wave. The lens-free device further includes a sensor positioned to detect an optical signal caused by illuminating the cells. The substrate surface includes a microelectrode array for sensing an electrophysiological signal from the cells.

A method for analyzing microorganisms arranged in a sample is provided, the sample including a viability marker to modify an optical property of the microorganisms in different ways depending on whether they are dead or alive, the method including illumination of the sample and acquisition of an image of the latter by an image sensor, the image sensor then being exposed to an exposure light wave; determining positions of different microorganisms from the acquired image; applying a propagation operator to calculate at least one characteristic value of the exposure light wave at each radial position and at a plurality of distances from the detection plane representing a change in the characteristic value between the image sensor and the sample; and identifying living microorganisms according to each profile.

An apparatus for in-line holographic imaging is disclosed. In one aspect, the apparatus includes at least a first light source and a second light source arranged for illuminating an object arranged in the apparatus with a light beam. The apparatus also includes an image sensor arranged to detect at least a first and a second interference pattern, wherein the first interference pattern is formed when the object is illuminated by the first light source and the second interference pattern is formed when the object is illuminated by the second light source. The first and second interference patterns are formed by diffracted light, being scattered by the object, and undiffracted light of the light beam. The at least first and second light sources are arranged at different angles in relation to the object, and possibly illuminate the object using different wavelengths.

Embodiments described herein relate to a large area lens-free imaging device. One example is a lens-free device for imaging one or more objects. The lens-free device includes a light source positioned for illuminating at least one object. The lens-free device also includes a detector positioned for recording interference patterns of the illuminated at least one object. The light source includes a plurality of light emitters that are positioned and configured to create a controlled light wavefront for performing lens-free imaging.

Provided is a digital holographic imaging apparatus, comprising: an illumination portion (10) having an illumination light emission surface (32i) for emitting coherent light of a specific wavelength as illumination light toward an object (1) side relative to the illumination light emission surface (32i), and a reference light emission surface (32r) for emitting the coherent light, as reference light, in a direction opposite to the illumination light; and an image sensor (50) located on the reference light emission surface (32r) side of the illumination portion (10) and imaging an interference pattern between object light having been modulated by the object (1) and passed through the illumination portion (10) and the reference light of the illumination light, the image sensor (50) having a pixel array (51) comprising two-dimensionally aligned pixels.

Impurities within a sample are detected by use of holographic video microscopy. The sample flows through the microscope and holographic images are generated. The holographic image is analyzed to identify regions associated with large impurities in the sample. The contribution of the particles of the sample to the holographic images is determined and the impurities are characterized.

A method and apparatus for monitoring particulate concentrations in ambient air use digital in-line holography and automated digital algorithms to classify and determine mass density and other characteristics of particles within a determined mass and size range. An embodiment provides a sampling plate on which particles are deposited at locations which depend on sizes and masses of the particles. A digital in-line hologram of the sampling plate is processed to obtain information about the particles. The method and apparatus have example application to environmental monitoring.

According to a first aspect, there is provided a method of holographic wavefront sensing, the method including: receiving a light beam, which has a wavefront to be analyzed, on a transparent, flat substrate, which is provided with a lattice of opaque dots, wherein the substrate is arranged above an image sensor; detecting by the image sensor an interference pattern formed by diffracted light, being scattered by the opaque dots, and undiffracted light of the light beam received by the image sensor; processing the detected interference pattern to digitally reconstruct a representation of a displaced lattice of opaque dots, which would form the interference pattern on the image sensor upon receiving the light with a known wavefront; and comparing the representation of the displaced lattice to a known representation of the lattice of opaque dots on the substrate to determine a representation of the wavefront form of the received light beam.

The present invention provides an ellipsometry device and an ellipsometry method whereby measurement efficiency can be enhanced. In this method, an object is illuminated by spherical-wave-like illumination light Q linearly polarized at 45 (S1), and an object light O, being a reflected light, is acquired in a hologram I.sub.OR using a spherical-wave-like reference light R having a condensing point near the condensing point of the illumination light Q, and a hologram I.sub.LR of the reference light R is furthermore acquired using a spherical-wave reference light L having the same condensing point as that of the illumination light Q (S2). The holograms are separated into p- and s-polarized light holograms I.sup.K.sub.OR, I.sup.K.sub.LR, =p, s and processed to extract object light waves, and object light spatial frequency spectra G.sup.K(u, v), =p, s are generated (S3) (S4). Ellipsometric angles (), () are obtained for each incident angle from the amplitude reflection coefficient ratio =G.sup.p/G.sup.s=tan .Math.exp(i). Through use of numerous lights having different incident angles included in the illumination light Q, data of numerous reflection lights can be acquired collectively in a hologram and can be processed.