Both a hologram and fluorescence are simultaneously captured in a state in which they can be reconstructed separately. A recording device (10) includes: a laser light source (LS1) which irradiates a subject (13) with object illumination light so that object light is generated; and an image capturing device (12) which captures (i) a hologram formed by interference between reference light and object light and (ii) an image of fluorescence, and the object illumination light further excites a fluorescent material (14) contained in the subject (13).

The present invention relates to an arrangement for the generation of images of optical sections of a three-dimensional (3D) volume in space such as an object, scene, or target, comprising: an illumination unit, an optical arrangement for the imaging of the object onto at least one spatially resolving detector, a scanning mechanism for scanning the entire object and a signal processing unit for the implementation of a method for digital reconstruction of a three-dimensional representation of the object from images of said object as obtained by said detector (which may be in a form of a hologram), wherein the optical arrangement includes a diffractive optical element (herein a phase pinhole), realized using a Spatial Light Modulator (SLM) configured to mimic an actual physical pinhole, while allowing the formation of a three-dimensional representation for a specific point of interest in said object, such that for each scanning position a single hologram or an image is recorded.

A method of obtaining a focus term by using a periodicity of the focus term is provided. The focus term may be used in a plurality of operation processes for processing image data.

A new optical arrangement that creates high efficiency, high quality Fresnel Incoherent Correlation Holography (FINCH) holograms using transmission liquid crystal GRIN (TLCGRIN) diffractive lenses has been invented. This is in contrast to the universal practice in the field of using a reflective spatial light modulator (SLM) to separate sample and reference beams. Polarization sensitive TLCGRIN lenses enable a straight optical path, have 95% transmission efficiency, are analog devices without pixels and are free of many limitations of reflective SLM devices. An additional advantage is that they create an incoherent holographic system that is achromatic over a wide bandwidth. Two spherical beams created by the combination of a glass and a polarization sensitive TLCGRIN lenses interfere and a hologram is recorded by a digital camera. FINCH configurations which increase signal to noise ratios and imaging speed are also described.

A method for digitally generating a hologram plane from a three-dimensional scene, cut into a plurality of planes parallel to the hologram plane. The method includes for a current plane: counting a number of points of the non-zero amplitude scene; choosing a first or second technique for propagating a light wave emitted by the current plane as a function of a number of points of non-zero amplitude included in the current plane and with a preset threshold value, the first, point-based technique calculating the propagation of a sum of light waves emitted by point sources constituted by the points of the scene portion of a non-zero amplitude of the current plane on a following plane, and the second, field-based technique, globally calculating a light wave emitted by the scene portion situated in the current plane on a given plane; and processing the current plane according to the chosen propagation technique.

Provided is a digital holographic imaging apparatus, comprising: an illumination portion (10) having an illumination light emission surface (32i) for emitting coherent light of a specific wavelength as illumination light toward an object (1) side relative to the illumination light emission surface (32i), and a reference light emission surface (32r) for emitting the coherent light, as reference light, in a direction opposite to the illumination light; and an image sensor (50) located on the reference light emission surface (32r) side of the illumination portion (10) and imaging an interference pattern between object light having been modulated by the object (1) and passed through the illumination portion (10) and the reference light of the illumination light, the image sensor (50) having a pixel array (51) comprising two-dimensionally aligned pixels.

Provided is a digital holographic image-taking apparatus, including: an illumination portion (10) having a light emission surface (14D) for emitting illumination light toward an object (1), the illumination light having a specific wavelength in a coherent plane waveform; and an image sensor (50) having an pixel array (51) including two-dimensionally arranged pixels, the image sensor (50) capturing an interference pattern generated based on the illumination light having acted on the object (1), in which the following conditional expression is satisfied: 0.0000001<Z.sup.2/S<16, where S represents the area of the light emission surface (14D), and Z represents the distance from the light emission surface (14D) to the pixel array (51).

A holographic characterization and playback apparatus is provided, which includes a light source, an optical path-forming optical system for separating the light emitted from the light source into a probe light and a reference light of different polarizations, and combining optical paths of the probe light and the reference light.

A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

A method, an interferometer, and a signal processing device, each for determining an input phase and/or an input amplitude of an input light field, are disclosed. Here, an input light field is divided into a first light field and a second light field by amplitude splitting. The first light field and the second light field are propagated such that the propagated second light field is defocused relative to the propagated first light field. The propagated first light field is superimposed on the propagated light field and caused to interfere.