A system and method to produce a hologram of a single plane of a three dimensional object includes an electromagnetic radiation assembly to elicit electromagnetic radiation from a single plane of said object, and an assembly to direct the elicited electromagnetic radiation toward a hologram-forming assembly. The hologram-forming assembly creates a hologram that is recorded by an image capture assembly and then further processed to create maximum resolution images free of an inherent holographic artifact.

In a microscopic observation unit (10), hologram data is acquired at each measurement position on a cell culture plate (13) while a light-source section (11) and other related sections are gradually moved by a moving section (15). Every time a set of data for one measurement position is acquired, a measurement monitoring image creator (24) creates a thumbnail image by reducing the size of a hologram image which is based on original data (two-dimensional distribution of light intensity). A display processor (25) pastes the create thumbnail image to progressively complete the hologram image of the entire plate to be displayed on a display unit (27). A measurement operator watches the hologram image during the execution of the measurement. When it has been concluded that the ongoing measurement is inappropriate, the operator presses a measurement stop button to immediately discontinue the measurement. Thus, When there is a problem with the measurement, such as a foreign object mixed in the sample, the measurement can be discontinued before a phase image or intensity image based on the hologram data is reconstructed on the server after the completion of the entire measurement, so as to avoid wasting time for the useless measurement.

A method for observing a sample by lensless imaging, in which a sample is positioned between a laser diode and an image sensor, the laser diode being supplied with a supply current whose intensity is less than or equal to a critical value. This critical intensity is determined during preliminary operations, during which the intensity is initially greater than a laser threshold of the diode. By observing the image formed at the image sensor, the intensity is decreased until an attenuation of the interference images on the formed image is observed, the critical intensity corresponding to the intensity at which this attenuation is optimum.

An illumination unit emits illumination light to a specimen. An image sensor includes multiple pixels arranged in a two-dimensional manner on a photoelectric surface. The image sensor captures an image of a magnitude distribution of an interference pattern formed due to the illumination light that has interacted with the specimen. A limiter limits at least one from among the spatial frequency of the interference pattern formed on the photoelectric surface and the incident angle of the light input to the photoelectric surface.

Provided are an improved holographic reconstruction apparatus and method. A holographic reconstruction method includes: obtaining an object hologram of a measurement target object; generating a digital reference hologram calculated from the obtained object hologram; extracting each of a first phase information of the object hologram and a second phase information of the calculated digital reference hologram; calculating a phase information difference from the first phase information of the object hologram and the second phase information of the calculated digital reference hologram; and compensating for distorted phase information based on the calculated phase information difference, and calculating quantitative thickness information of the measurement target object by using the compensated distorted phase information to reconstruct 3-dimensional (3D) shape information and quantitative thickness information of the measurement target object.

The present invention includes method and device for label-free holographic screening and enumeration of tumor cells in bulk flow comprising: a laser source, a micro-objective, a pinhole device and a collimating lens, a mirror, a sample chamber with a sample flow inlet on a first side of the sample chamber and a sample flow outlet connected by a microchannel, and a detector, wherein the collimated laser beam passes through microchannel and interacts with cells in the sample to generate a respective hologram at the detector, wherein a processor calculates a numerical reconstruction from the respective hologram and generates a focused image of the numerous cells using the numerical reconstruction, wherein the numerous cells are enumerated by looking at a size, a maximum intensity and a mean intensity of the focused image.

A visualization system includes an optical recording unit configured to capture optical signals characterizing at least one partial region of an object, a 3D reconstruction unit configured to ascertain spatial data sets, which describe the partial region of the object, based on the captured optical signals, a hologram computational unit configured to ascertain control data for producing a holographic presentation based on the spatial data sets of the partial region of the object, and a visualization unit configured to visualize a holographic presentation of the at least one partial region of the object for a user of the visualization system based on the control data. In addition, a suitable method for producing holographic presentations from optical signals is provided.

Systems and methods are provided for a digital holography range Doppler receiver. The subject system transmits outgoing electromagnetic radiation to a target, and provides a first reference local oscillator (LO) beam to a first detector and a second reference LO beam to a second detector, based on the outgoing electromagnetic radiation. The system receives reflected electromagnetic radiation from the target through a first optical receiver and a second optical receiver having a smaller diameter, and determines range and velocity of the target simultaneously using an interference with the second reference LO beam. The system applies time and frequency offsets to the first reference LO beam based on the measured range and velocity to align the first reference LO beam with the reflected electromagnetic radiation, and produces an image of the target using the first reference LO beam having the applied time and frequency offsets.

A lensless holographic imaging system having a holographic optical element includes: a coherent light source for outputting a first light beam and a second light beam, wherein the first light beam irradiates a first inspection plane to form first object-diffracted light; a light modulator for modulating the second light beam into reading light having a specific wavefront; a multiplexed holographic optical element, wherein the first object-diffracted light passes through the multiplexed holographic optical element, and the reading light is input into the multiplexed holographic optical element to generate a diffracted light beam as system reference light; and an image capture device for reading at least one interference signal generated by interference between the first object-diffracted light and the system reference light. The lensless holographic imaging system has a relatively small volume and relatively high diffraction efficiency.

The disclosed subject matter relates to a method for determining a three-dimensional particle distribution in a medium, comprising: emitting a coherent light beam to irradiate the sample; recording an interference image of the scattered light beam and a second part of the light beam that has not been scattered; computing, from the interference image, for each one of a plurality of virtual planes lying within the sample, a reconstructed image of the sample, generating for each reconstructed image, a presence image, wherein a value is assigned to each pixel of the presence images if the corresponding pixel of the reconstructed image has an intensity value exceeding a threshold value and if the corresponding pixel of the reconstructed image has a phase value with a predetermined sign.