The present disclosure provides a method and apparatus for generating a full-color holographic image. The method of generating a full-color holographic image includes forming images for each color channel based on complex hologram data extracted from rays propagating from a target object, and combining the formed images into one color image, wherein the images for each color channel are formed at reconstruction points for each color channel derived based on the complex hologram data.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

An electromagnetic wave phase/amplitude generation device includes a radiation unit configured to radiate electromagnetic waves of a random radiation pattern on a spatial frequency in which a state of the electromagnetic waves to be radiated for each divided region is determined to an imaging object, an imaging unit configured to generate a captured image by imaging scattered electromagnetic waves that are electromagnetic waves generated when the imaging object scatters the electromagnetic waves of the radiation pattern radiated by the radiation unit, and a generation unit configured to generate information indicating at least a phase and amplitude of the electromagnetic waves from the imaging object by performing an arithmetic sparsity constraint operation according to sparsity of the imaging object on the basis of the captured image generated by the imaging unit, information indicating the radiation pattern, and information indicating a signal of the imaging object.

An electromagnetic wave phase/amplitude generation device includes a radiation unit configured to radiate electromagnetic waves of a random radiation pattern on a spatial frequency in which a state of the electromagnetic waves to be radiated for each divided region is determined to an imaging object, an imaging unit configured to generate a captured image by imaging scattered electromagnetic waves that are electromagnetic waves generated when the imaging object scatters the electromagnetic waves of the radiation pattern radiated by the radiation unit, and a generation unit configured to generate information indicating at least a phase and amplitude of the electromagnetic waves from the imaging object by performing an arithmetic sparsity constraint operation according to sparsity of the imaging object on the basis of the captured image generated by the imaging unit, information indicating the radiation pattern, and information indicating a signal of the imaging object.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

A device in holographic imaging comprises: at least two light sources, wherein each of the at least two light sources is arranged to output light of a unique wavelength; and at least one holographic optical element, wherein the at least two light sources and the at least one holographic optical element are arranged in relation to each other such that light from the at least two light sources incident on the at least one holographic optical element interacts with the at least one holographic optical element to form wavefronts of similar shape for light from the different light sources.

A method is presented for determining a phase of an input beam (110, E.sub.in) without a reference ray. In the method, an input beam (110, E.sub.in) having a plurality of input rays is split into a main beam (112, E1) and a reference beam (114, E2) in such a way that each input ray is split into a main ray of the main beam (112, E1) and a comparative ray of the reference beam (114, E2). The main beam (112, E1) is propagated along a first interferometer arm, and the reference beam (114, E2) is propagated along the second interferometer arm. The propagated main beam (112, E1) and the propagated reference beam (114, E2) are superposed to form an interference beam having a plurality of interference rays. The propagation along the first and second interferometer arms is carried out such that at least one interference ray of the interference beam is a superposition of a main ray of the propagated main beam (112, E1) assigned to a first input ray of the input beam (110, E.sub.in), and of a comparative ray of the propagated reference beam (114, E2) assigned to a second input ray of the input beam (110, E.sub.in) different from the first input ray.

The present disclosure provides a method for calculating a light field distribution in the process of generating a computer-generated hologram, including: performing a three-dimensional modeling to an object for which a hologram is to be generated so as to obtain a three-dimensional model of the object; determining the luminous characteristics of each voxel of the three-dimensional model at various azimuth angles within a viewing angle range of the hologram to be formed; and calculating a light field distribution of the object light wave of each voxel on the holographic plane based on the luminous characteristics of each voxel at various azimuth angles within the viewing angle range of the hologram to be formed. The present disclosure also provides a method and a system for generating a computer-generated hologram.

A method for observing a sample includes illuminating the sample with a light source and forming a plurality of images, by an imager, the images representing the light transmitted by the sample in different spectral bands. From each image, a complex amplitude representative of the light wave transmitted by the sample is determined in a determined spectral band. The method further includes backpropagation of each complex amplitude in a plane passing through the sample, determining a weighting function from the back-propagated complex amplitudes, propagating the weighting function in a plane along which the matrix photodetector extends, updating each complex amplitude, in the plane of the sample, according to the weighting function propagated.

There is provided a device allowing a sample to be observed in a first mode, by lensless imaging using a first sensor. The first mode allows a first image to be obtained, on the basis of which a region of interest of the sample may be identified. The device then allows, via a relative movement, the region of interest to be analyzed using a more precise second mode and in particular using an optical system coupled to a second sensor.