The present disclosure relates to improved holographic reconstruction device and a method. In one aspect, the present disclosure relates to improved holographic reconstruction device and method that can measure a digital hologram regardless of optical characteristics of an object to be measured, by an all-in-one type system integrating a transmissive system that measures an object transmitting light and a reflective system that measures an object reflecting light.

The present disclosure presents systems, apparatuses, and methods of holographic imaging. In this regard, a method comprises transmitting light and illuminating a semi-transparent sample object; and forming, at a hologram plane, an interference pattern of a real image of the sample object from a scattered object beam and an unscattered reference beam from the transmitted light. To do so, the scattered object beam and the unscattered reference beam are in-line with one another, and a distance between the hologram plane to the sample object is set at a distance that substantially weakens a virtual image of the sample object formed from the scattered object beam and the unscattered reference beam. Accordingly, the method further comprises recording the interference pattern of a hologram formed from the scattered object beam and the unscattered reference beam at a detector; and reconstructing a 3D optical field of the hologram without phase retrieval.

Method for obtaining an image of a sample (10), comprising: a) illuminating the sample using a light source (11); b) acquiring, using an image sensor (16), a first image (I.sub.1,P0) of the sample (10), said image being formed in the detection plane (P.sub.0), the first image being representative of an exposure light wave (14) propagating, from the sample, to the image sensor, along a first optical path (L.sub.1);
the method comprising, following b) c) modifying an optical refractive index, between the image sensor and the sample; d) following c), acquiring a second image (I.sub.2,P0) of the sample, said image being representative of the exposure light wave (14) along a second optical path (L.sub.2); e) implementing an iterative algorithm that combines the first and second images so as to obtain an image of the sample.

The present disclosure relates to improved holographic reconstruction device and a method. In one aspect, the present disclosure relates to improved holographic reconstruction device and method that can measure a digital hologram regardless of optical characteristics of an object to be measured, by an all-in-one type system integrating a transmissive system that measures an object transmitting light and a reflective system that measures an object reflecting light.

The present invention relates to a method of detecting a possible infection of malaria in a patient using a digital optical microscope.

A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term and backward gradient descent (110) on the regularization term.

To obtain a more accurate image by improving a utilization efficiency of light energy while at the same time suppressing with a simpler method distortion that may occur in an inline hologram when a plurality of lights having different wavelengths are used, an observation device (1) according to the present disclosure includes a light source part (11) in which a plurality of light emitting diodes (101) having different light emission wavelengths with a length of each light emission point being smaller than 100λ (λ: light emission wavelength) are arranged such that a separation distance between the adjacent light emitting diodes is equal to or smaller than 100λ (λ: light emission wavelength); and an image sensor (13) installed so as to be opposed to the light source part with respect to an observation target object.

An in-line holographic microscope can be used to analyze a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. An assay using the holographic microscope for holographic particle characterization directly detect viruses, antibodies and related targets binding to the surfaces of specifically functionalized micrometer-scale colloidal probe beads. The system detects binding of targets by directly measuring associated changes in the bead's diameter without the need for downstream labeling and analysis.

A device and a method for observing an object by imaging, or by lensless imaging. The object is retained by a holder defining an object plane inserted between a light source and an image sensor, with no enlargement optics being placed between the object and the image sensor. An optical system is arranged between the light source and the holder and is configured to form a convergent incident wave from a light wave emitted by the light source, and for forming a secondary light source, conjugated with the light source, positioned in a half-space defined by the object plane and including the image sensor, such that the secondary source is closer to the image sensor than to the holder. This results in an image with a transversal enlargement factor having an absolute value of less than 1.

This disclosure discloses a method of creating a three-dimensional image of a sample using snapshot optical tomography. The method includes generating a plurality of beams incident on the sample simultaneously, acquiring a field image at a plane not conjugate to the sample plane using off-axis digital holography, extracting amplitude data and phase data for the field image, restoring the sharpness by backpropagating the field image using the extracted amplitude and phase data, acquiring a background image, extracting amplitude data and phase data for the background image, and reconstructing a three-dimensional image of the sample with the backpropagated field image and the background image. The method also includes arranging more than one imaging chains to remove the missing angle artefacts in optical tomography. Also disclosed are systems for performing the method.