A method of training AI for label-free cell viability determination includes a step of providing a cell sample, a step of obtaining a fluorescence image and a DHM image of the cell sample, a step of determining a first cell viability of the cell sample according to the fluorescence image of the cell sample, a step of labeling the DHM image of the cell sample as a model specifying the first cell viability, and a step of performing AI training by using the model containing the DHM image of the cell sample.

A holographic imaging system may include an optical source configured to output a source beam, a splitter configured to split the source beam into a reference beam and an object beam that is incident on a target to form a scattered object beam, and a pre-filter comprising a telecentric lens and a spatial filter. The pre-filter may be configured to receive the scattered object beam and filter diffuse light from the scattered object beam to form a filtered scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, and an imaging array configured to receive the interference beam and generate raw holographic data based on the interference beam.

An ultrasound emitter launches an ultrasonic signal into a diffuse medium such as tissue. The diffuse medium is illuminated with an infrared illumination signal. activating an ultrasound emitter to launch an ultrasonic signal into a diffuse medium. An infrared reference beam is interfered with an infrared exit signal having an infrared wavelength that is the same as the infrared illumination signal. An infrared image is captured of the interference of the infrared reference beam with the infrared exit signal.

A method comprises obtaining input data comprising a hologram of a 3-dimensional (3D) particle field, a depth map of the 3D particle field, and a maximum phase projection of the 3D particle field. The method also comprises applying a U-net convolutional neural network (CNN) to the input data to generate output data. Encoder blocks have residual connections between a first layer and a second layer that skips over a convolution layer of the encoder block. Decoder blocks have residual connections between a first layer and a second layer that skips over a convolution layer of the decoder block. The output data includes a channel in which pixel intensity corresponds to relative depth of particles in the 3D particle field and an output image indicating locations of centroids of the particles in the 3D particle field.

An advance in high-resolution optical metrology has been achieved by the introduction of incoherent holographic imaging. FINCH, an example of incoherent holography, is shown to simplify the process, eliminating many steps in metrology and at the same time increasing throughput, resolution and accuracy of the method. A proposed technique requires only a single image capture with a non-moving camera rather than the capture of multiple stacks of images requiring many camera exposures and movement of the camera or sample in the conventional techniques.

The present invention relates to an inline scanning holography system for a phosphor and a transmitter. According to the present invention, the inline scanning holography system includes a polarization sensitive lens that receives a linearly polarized beam and generates a first spherical wave of right-handed circular polarized light having a negative focal length and a second spherical wave of left-handed circular polarized light having a positive focal length, a polarizer that passes only a beam component in a predetermined polarization direction therethrough among components of the generated first and second spherical waves, a scanning unit for scanning a phosphor by using an interference beam generated between the first and second spherical waves passing through the polarizer, and a first photodetector that detects a fluorescent beam diverged from the phosphor. According to the present invention, a high-efficiency and high-quality optical scanning holography for a phosphor or a transmitter may be implemented.

The inventive concept relates to a device for detecting particles in air, said device comprising a receiver for receiving a flow of air comprising particles, a sample carrier, and a particle capturing arrangement. The particle capturing arrangement is configured to separate the particles from the flow of air for and to collect a set of particles on a surface of the sample carrier. The device further comprises a light source configured to illuminate the particles on the sample carrier, such that an interference pattern is formed by interference between light being scattered by the particles and non-scattered light from the light source. The device further comprises an image sensor configured to detect the interference pattern. The device further comprises a cleaner configured for cleaning the surface of the sample carrier for enabling re-use of the surface for collection of a subsequent set of particles.

A holographic imaging device and method realizes both a transmission type and a reflection type, and also realizes a long working distance wide field of view or ultra-high resolution. Object light emitted from an object, sequentially illuminated with parallel illumination light whose incident direction is changed, is recorded on a plurality of object light holograms for each incident direction using off-axis spherical wave reference light. The reference light is recorded on a reference light hologram using in-line spherical wave reference light being in-line with the object light. An object light wave hologram and its spatial frequency spectrum at the object position are generated for each incident direction using each hologram. A synthetic spectrum which occupies a wider frequency space is generated by matching each spectrum in the overlapping area, and a synthetic object light wave hologram with increased numerical aperture is obtained thereby.

A method of generating a hologram includes receiving an input image representing a 3D object, defining a first phase value for a first pixel data such that spatio-temporally identical pixels with respect to the input image have the same phase, defining a second phase value for a second pixel data such that spatio-temporally identical pixels with respect to the input image have the same phase, and generating a multi-view hologram using the first phase value and the second phase value.

When the optical system is illuminated with an illumination light flux emitted from one extant input image point, an interference image generated by superimposing an extant output light flux output from the optical system and a reference light flux coherent with the extant output light flux is imaged to acquire interference image data, and thus to acquire measured phase distribution, and this acquisition operation is applied to each extant input image point. Thus, each measured phase distribution is expanded by expanding functions μn(u, v) having coordinates (u, v) on a phase defining plane as a variable to be represented as a sum with coefficients Σn{Ajn.Math.μn(u, v)}. When the optical system is illuminated with a virtual illumination light flux, a phase Ψ(u, v) of a virtual output light flux is determined by performing interpolation calculation based on coordinates of a virtual light emitting point.