A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

Embodiments are directed to an apparatus for creating a scene comprising: a plurality of micro-mirrors configured to rotate between an off position and at least two on positions to generate a plurality of holograms, and a processor configured to select positions for the micro-mirrors based on an input specification of the scene.

A method and system of imaging a moving object within a microfluidic environment includes illuminating a first side of a flow cell configured to carry the moving object within a flow of carrier fluid with an illumination source emitting at least partially coherent light, the at least partially coherent light passing through an aperture prior to illuminating the flow cell. A plurality of lower resolution frame images of the moving object are acquired with an image sensor disposed on an opposing side of the flow cell, wherein the image sensor is angled relative to a direction of flow of the moving object within the carrier fluid. A higher resolution image is reconstructed of the moving object based at least in part on the plurality of lower resolution frame images.

A holographic projector comprises an image processing engine, a hologram engine, a display engine and a light source. The image processing engine is arranged to receive a source image for projection and generate a plurality of secondary images from a primary image based on the source image. The source image comprises pixels. Each secondary image may comprise fewer pixels than the source image. The plurality of secondary images are generated by sampling the primary image. The hologram engine is arranged to determine, such as calculate, a hologram corresponding to each secondary image to form a plurality of holograms. The display engine is arranged to display each hologram on the display device. The light source is arranged to Illuminate each hologram during display to form a holographic reconstruction corresponding to each secondary image on a replay plane. The primary image is selected from the group comprising: the source image and an intermediate image

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

The present disclosure provides an apparatus and method for fabricating optical devices. The apparatus includes a support table having process chambers and a laser used to direct a beam along a propagation path to each of the process chambers. A central mirror is centrally disposed among the process chambers and is rotatable to reflect the beam to each of the process chambers for processing. A beam splitter is disposed within each of process chambers, each beam splitter is used to receive beams from the central mirror and emits a first beam in a first direction and a second beam in a second direction. A first mirror directs the first beam to a device and a second mirror directs the second beam to the device. Each of the first and second mirror is rotatable in at least three axes.

An image reproduction device reproduces an image including N different parameters of a wavelength range or the like, and includes: a multiple hologram acquisition part that acquires N to 2N multiple holograms obtained by multiplex-recording interference patterns for each parameter; a parameter selection part that selects the parameters one by one; a hologram generation part that generates a computer generated hologram containing two lightwaves having the selected parameter, from the multiple hologram; and a lightwave restoration part that restores one of the two lightwaves from the computer generated hologram.

Techniques for holographic display by modulating optical images in amplitude and phase via a layer of liquid crystals are described. According to one aspect of the techniques, a voltage being applied or coupled across the layer of liquid crystals is controlled by gradually increasing the voltage from a low level to a high level to perform the AM in a first range and the PM in second range, where the characteristics of the liquid crystals is significant, for example, by increasing the thickness or optical birefringence of the layer of liquid crystals.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

An optical device (100) for forming a distribution of a three-dimensional light field comprises: an array (102) of unit cells (104), a unit cell (104) being individually addressable for switching the optical property of the unit cell (104) between a first and a second condition; wherein the unit cells (104) are configured to be selectively active or inactive and wherein the array (102) comprises at least a first and a second disjoint subset (110; 112; 114; 116), and wherein the unit cells (104) in a subset (110; 112; 114; 116) are configured to be jointly switched from inactive to active, wherein the active unit cells (104) are configured to interact with an incident light beam (106) for forming the distribution of the three-dimensional light field; and wherein the optical device (100) is configured to address inactive unit cells (104) for switching the optical property of unit cells (104).