A method of calibration of a device for analyzing at least one element present in a sample, said device including: a detection assembly configured to acquire an image formed by the interference between a light source and said sample; and digital processing means configured to detect a digital position of at least one element in said sample based on said acquired image; said calibration method including the implementation of a plurality of predetermined displacements of said sample with respect to said detection assembly and, for all of said displacements, the detection of a digital position of a same element to determine the digital position and the real position matching model according to the predetermined displacements and to the digital positions of said element after each displacement.

Techniques for holographic display by modulating optical images in amplitude and phase via a layer of liquid crystals are described. According to one aspect of the techniques, a voltage being applied or coupled across the layer of liquid crystals is controlled by gradually increasing the voltage from a low level to a high level to perform the AM in a first range and the PM in second range, where the characteristics of the liquid crystals is significant, for example, by increasing the thickness or optical birefringence of the layer of liquid crystals.

Apparatuses and methods for comparative holographic imaging to improve structural and molecular information of reconstructions is disclosed herein. An example method at least includes acquiring a plurality of holograms of a sample, wherein each hologram of the plurality of holograms is acquired at a different electron beam energy, and determining atomic and structural information of the sample based at least on a comparison of at least two of the holograms of the plurality of holograms.

An embodiment of the invention relates to a method for carrying out a time-resolved interferometric measurement comprising the steps of generating at least two coherent waves, overlapping said at least two coherent waves and producing an interference pattern, measuring the interference pattern for a given exposure time, thereby forming measured interference values, and analyzing the measured interference values and extracting amplitude and/or phase information from the measured interference values. In at least one time segment, hereinafter referred to as disturbed time segment, of the expo-sure time, the interference pattern is intentionally disturbed or destroyed such that the corresponding measured interference values describe a disturbed or destroyed interference pattern. In at least one other time segment, hereinafter referred to as undisturbed time segment, of the exposure time, the interference pattern is undisturbed or at least less disturbed compared to the disturbed time segment such that the corresponding measured interference values describe an undisturbed or less disturbed interference pattern. The measured interference values that were measured during the entire given exposure time, are filtered, wherein those interference values that were measured during the at least one disturbed time segment, are reduced, suppressed or discarded. The filtered interference values are analyzed and the amplitude and/or phase information is extracted from the filtered interference values.

A system for generating multi-depth image sequence comprising a modulation array. The modulation array comprising a plurality of light modulators which may shift light incident upon the modulators by a number of degrees. The plurality of light modulators may shift light in concert according to a modulation shift pattern. The modulation shift pattern can be configured to focus incident light to a voxel or to form a 3-D image. One or more modulation shift patterns can be changed or cycled through to raster one or more image objects in one or more image depth planes.

There are provided holographic optical elements (HOEs) and methods of making the same. An example of such methods includes recording a first hologram in a contiguous holographic recording medium of the HOE. The first hologram may receive a beam of light and direct at least a portion of the beam into a light guide to form an incoupled beam. The method also includes recording a second hologram in the contiguous holographic recording medium. The second hologram may receive at least a portion of the incoupled beam and direct the portion of the incoupled beam out of the light guide to form an outcoupled beam. In addition, the method includes affixing the holographic recording medium to the light guide.

Techniques for modulating both amplitude and phase via a layer of liquid crystals are described. According to one aspect of the techniques, a voltage being applied or coupled across the layer of liquid crystals is controlled by gradually increasing the voltage from a low level to a high level to perform the AM in a first range and the PM in second range, where the characteristics of the liquid crystals is significant, for example, by increasing the thickness or optical birefringence of the layer of liquid crystals.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

In a microscopic observation unit (10), hologram data is acquired at each measurement position on a cell culture plate (13) while a light-source section (11) and other related sections are gradually moved by a moving section (15). Every time a set of data for one measurement position is acquired, a measurement monitoring image creator (24) creates a thumbnail image by reducing the size of a hologram image which is based on original data (two-dimensional distribution of light intensity). A display processor (25) pastes the create thumbnail image to progressively complete the hologram image of the entire plate to be displayed on a display unit (27). A measurement operator watches the hologram image during the execution of the measurement. When it has been concluded that the ongoing measurement is inappropriate, the operator presses a measurement stop button to immediately discontinue the measurement. Thus, When there is a problem with the measurement, such as a foreign object mixed in the sample, the measurement can be discontinued before a phase image or intensity image based on the hologram data is reconstructed on the server after the completion of the entire measurement, so as to avoid wasting time for the useless measurement.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.