A method and system for identifying a Raman spectrum component of an observed spectrum is provided. The observed spectrum is produced by interrogating a material such as a tissue sample with light at the one or more predetermined wavelengths, and the observed spectrum includes a background fluorescence component representative of fluorescent emissions resulting from the light interrogation and a Raman spectrum component representative of a Raman scattering resulting from the light interrogation. The method includes a) creating a reconstructed fluorescence spectrum representative of the background fluorescence component of the observed spectrum using one or more empirically determined fluorescent spectral profiles; and b) identifying the Raman spectrum of the observed spectrum using the reconstructed fluorescence spectrum.

A method and system for identifying a Raman spectrum component of an observed spectrum is provided. The observed spectrum is produced by interrogating a material such as a tissue sample with light at the one or more predetermined wavelengths, and the observed spectrum includes a background fluorescence component representative of fluorescent emissions resulting from the light interrogation and a Raman spectrum component representative of a Raman scattering resulting from the light interrogation. The method includes a) creating a reconstructed fluorescence spectrum representative of the background fluorescence component of the observed spectrum using one or more empirically determined fluorescent spectral profiles; and b) identifying the Raman spectrum of the observed spectrum using the reconstructed fluorescence spectrum.

A detection apparatus includes: a laser configured to emit laser light towards an object to be detected; a Raman spectrometer configured to receive a Raman light signal from the object; a light sensor configured to receive portions of the laser light reflected and scattered by the object under irradiation of the laser light, and to determine power of the received portions of the laser light; and a controller configured to control an operation of the detection apparatus based on the power determined by the light sensor. Further, there is provided a detection method using the above detection apparatus.

A spectrum measurement apparatus includes: a plurality of light sources configured to emit light having different wavelengths to an object; a light detector configured to receive light, which is reflected or scattered from or transmitted through the object, and to measure an intensity of the received light; and a processor configured to determine a strength of an electric signal to be applied to at least one of the plurality of light sources by using one of the plurality of light sources, and by applying the electric signal having the determined strength to the plurality of light sources to obtain a spectrum of the object.

A detection apparatus includes: a laser configured to emit laser light towards an object to be detected; a Raman spectrometer configured to receive a Raman light signal from the object; a light sensor configured to receive portions of the laser light reflected and scattered by the object under irradiation of the laser light, and to determine power of the received portions of the laser light; and a controller configured to control an operation of the detection apparatus based on the power determined by the light sensor. Further, there is provided a detection method using the above detection apparatus.

A spectrum measurement apparatus includes: a plurality of light sources configured to emit light having different wavelengths to an object; a light detector configured to receive light, which is reflected or scattered from or transmitted through the object, and to measure an intensity of the received light; and a processor configured to determine a strength of an electric signal to be applied to at least one of the plurality of light sources by using one of the plurality of light sources, and by applying the electric signal having the determined strength to the plurality of light sources to obtain a spectrum of the object.