Various embodiments are directed to a fluid composition sensor device and method of using the same. In various embodiments, the fluid flow composition sensor is configured to receive a volume of fluid, the fluid composition sensor comprising a housing, a removable fluid flow component, an impactor nozzle, a collection media assembly dock element configured to receive a replaceable collection media assembly comprising a collection media configured to receive one or more particles within the volume of fluid, an imaging device configured to capture an image of at least a portion of the one or more particles received by the fluid composition sensor, and a controller configured to determine, based at least in part on the image, at least one particle characteristic of the volume of fluid. The imaging device may be configured to capture the image of one or more particles received by the fluid composition sensor using lensless holography.

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

Various embodiments are directed to a fluid composition sensor device and method of using the same. In various embodiments, the fluid flow composition sensor is configured to receive a volume of fluid, the fluid composition sensor comprising a housing, a removable fluid flow component, an impactor nozzle, a collection media assembly dock element configured to receive a replaceable collection media assembly comprising a collection media configured to receive one or more particles within the volume of fluid, an imaging device configured to capture an image of at least a portion of the one or more particles received by the fluid composition sensor, and a controller configured to determine, based at least in part on the image, at least one particle characteristic of the volume of fluid. The imaging device may be configured to capture the image of one or more particles received by the fluid composition sensor using lensless holography.

A device includes a filter that enhances a beam profile of a received pulsed laser; a first optical element to direct the pulsed laser as a reference wave towards an optical sensor; an open cavity positioned between the first optical element and the optical sensor. The open cavity receives an aerosol particle, which enters the open cavity from any direction. The reference wave illuminates the aerosol particle. An illuminated particle generates and directs an object wave towards the optical sensor. A pixel array is connected to the optical sensor. The pixel array receives the reference wave and the object wave. The optical sensor creates a contrast hologram comprising an interference pattern of the illuminated particle. A processor creates an image of the illuminated particle based on the contrast hologram.

Various embodiments are directed to a device for detecting fluid particle characteristics comprising: a fluid composition sensor comprising: an illumination source configured to emit a light beam along a beam axis such that at least a portion of the light beam engages a collection media and illuminates particles disposed within a collection media; an imaging device configured to capture an image of the particles disposed within the collection media; and a light shield comprising: an internal shield portion enclosed within an inner surface of a shield wall; and one or more baffle elements disposed within the internal shield portion and configured to reflect at least a divergent portion of the light beam within the internal shield portion; wherein the light shield extends between the illumination source and the imaging device such that a portion of the light beam emitted along the beam axis passes through the internal shield portion.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

Laser speckle microrheology is used to determine a mechanical property of a biological tissue, namely, an elastic modulus. Speckle frames may be acquired by illuminating a coherent light and capturing back-scattered rays in parallel and cross-polarized states with respect to illumination. The speckle frames may be analyzed temporally to obtain diffuse reflectance profiles (DRPs) for the parallel-polarized and cross-polarized states. A scattering characteristic of particles in the biological tissue may be determined based on the DRPs, and a displacement characteristic may be determined based at least in part on a speckle intensity autocorrelation function and the scattering characteristic. A size characteristic of scattering particles may be determined based on the DRP for the parallel polarization state. The mechanical property may be calculated using the displacement and size characteristics.

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.