A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

A particle recovery device for recovering particles contained in a liquid sample, the particle recovery device comprising: a flow cell having a flow path through which the liquid sample flows; a density acquisition unit that acquires a density of the liquid sample; standing wave forming means that applies an ultrasonic wave into the flow path to generate a standing wave; a control unit that determines a frequency of the ultrasonic wave that generates the standing wave in the flow path based on the density acquired by the density acquisition unit and causes the standing wave forming means to apply the ultrasonic wave of the determined frequency; and recovery means that recovers particles focused in the flow path by the standing wave.

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements.

Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and can be contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

The present invention refers to a device for the analysis of a particle comprising an analysis chamber adapted to contain a positioning fluid. A parameter of the particle suspended in the positioning fluid is detected by means of a detection and control unit. A positioning unit, during a particle analysis operation, is activated and deactivated on the basis of the detected parameter of the particle. The detection and control unit can activate the at least one positioning unit so as to generate a temporary positioning flow in the positioning fluid, such that said temporary positioning flow acts directly on the particle and drives the position of the particle so as to move it into a predefined position in the analysis chamber. The detection and control unit can also deactivate the at least one positioning unit when the particle to be analyzed is in the predefined position, such that the positioning fluid is at rest.

Disclosed herein is a microfluidic device comprising, at least one sample inlet for receiving biological cells in a biological fluid sample; at least one sheath flow inlet for receiving a sheath fluid; at least one curvilinear channel configured to provide the biological fluid sample substantially in an outer flow and the sheath fluid in substantially an inner separated flow; a plurality of cell traps at the periphery of the curvilinear channel, each trap configured to admit a single cell having a targeted size range from the outer flow.

A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flow-cell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements.

Disclosed herein are methods for producing high density cellular arrays. In some embodiments, the methods comprise: providing a sample comprising a plurality of cells; and introducing the plurality of cells in the sample into microwells of a microwell array to produce a cellular array, wherein the microwell array comprises 500 or more microwells per inch.sup.2, and wherein 25% or more of the microwells of the cellular array comprise a single cell. The disclosed methods can be used for producing a high density synthetic particle array and a high density reagent array.