An imaging flow cytometer includes: a flow channel in which an observation object flows and a length in a width direction is longer than a length in a height direction; an acoustic element configured to apply acoustic waves as standing waves to the flow channel; a light source that irradiates the flow channel with illumination light; an image sensor configured to image at least a line included in a cross section of the observation object crossing a flow line direction which is a direction in which the observation object flows in the flow channel by measuring or imaging the observation object passing through a position irradiated with the illumination light; and circuitry configured to generate an image in which the observation object is scanned in the flow line direction on the basis of a plurality of captured images acquired by the imaging unit imaging the line in a time series.

A method for treating a biological sample, preferably a food sample which may contain one or more species of interest, including a step of decomplexification by acoustophoresis.

The invention relates to a method for setting a cell concentration and/or a particle concentration in a dispensing device by means of which a liquid sample can be discharged, wherein the cell concentration and/or the particle concentration is ascertained in one region of the dispensing device, and the cell concentration and/or particle concentration that has been ascertained is compared with a target value, and a force exerted on one cell and/or one particle is adjusted based on the result of the comparison.

Exemplary embodiments of the present disclosure provide for two-stage microfluidic devices using surface acoustic waves, methods of use thereof, methods of making, methods of focusing and separating particles, and the like.

The present invention is a method and apparatus for acoustic focusing hardware and implementations.

Described herein are microfluidic devices and methods that can separate and concentrate particles in a sample.

It is possible to reduce a burden on a user in performing a spectroscopic analysis of a liquid sample. There is provided an analysis cell that is detachable and replaceable with respect to an analysis unit and accommodates a liquid sample, the analysis cell including a first wall surface pair that is made of a material transmitting a light, and a second wall surface pair for propagating an ultrasonic wave to the accommodated liquid sample, in which a first wall surface and a second wall surface forming the second wall surface pair and facing each other, are formed of materials having different acoustic impedances.

A fluid device includes: a flow path through which a fluid flows; and an ultrasonic wave transmitter configured to transmit an ultrasonic wave to generate a standing wave to the fluid in the flow path along a first direction orthogonal to a flowing direction of the fluid. The ultrasonic wave transmitter is in contact with the fluid and faces an antinode region corresponding to any antinode in the standing wave.

A method of forming a multilayer aggregate of objects in a channel including a liquid, the method including: a) providing objects at first and second superposed regions of the channel, b) obtaining first and second aggregates of objects, optionally by applying transverse acoustic waves, preferably stationary waves, within each region to objects, and c) bringing the first and second aggregates into contact to form the multilayer aggregate of objects by submitting the first and second aggregates to: gravity in absence of acoustic waves, or to acoustic waves, optionally stationary waves, inducing displacement of the first and second aggregates toward each other.

Apparatus is provided featuring an acoustic driver and a transducer. The acoustic driver is configured to provide an acoustic driver signal having a frequency that can be adjusted to yield a given wavelength, which in turn, will selectively capture a particular particle size of particles in a fluid, mixture or process flow. The transducer is configured to respond to the acoustic driver signal and provide an acoustic signal having a standing wave at the frequency in order to yield the given wavelength that will selectively capture the particular particle size of the particles in the fluid, mixture or process flow, in order to determine the mass of the particles having the particular particle size in the fluid, mixture or process flow.