Methods for evaluating flow cytometer data for the presence of a coincident event are provided. Methods of interest include receiving flow cytometer data associated with a first particle, where the received flow cytometer data is obtained from a detector following the irradiation of the first particle and a second particle in a flow stream by first and second light sources, respectively. Methods also include receiving first and second trigger signals associated with the first and second particles, respectively. In addition, the subject methods include generating a time interval for the first and second particles based on a difference between the first and second trigger signals, and evaluating the flow cytometer data for the presence of a coincident event based on the generated time interval by determining if the first and second particles are irradiated by the first and second light sources simultaneously. Systems and computer-readable media are also provided.

Provided are a microparticle sorting device, and a method and a program for sorting microparticles capable of stabilizing sorting performance over a prolonged period of time. The microparticle sorting device includes an imaging element and a controller. The imaging element obtains an image of fluid and fluid droplets at a position where the fluid discharged from an orifice which generates a fluid stream is converted into the fluid droplets. The controller controls driving voltage of an oscillation element which gives oscillation to the orifice and/or controls a position of the imaging element based on a state of the fluid in the image and/or a state of a satellite fluid droplet. The satellite fluid droplet does not include microparticles and exists between the position, where the fluid is converted into the fluid droplets, and a fluid droplet, among fluid droplets including the microparticles, which is closest to the position where the fluid is converted into the fluid droplets.

Aspects of the present disclosure include methods for array binning data signals from a photodetector (e.g., in a flow cytometer). Methods according to certain embodiments include detecting light with a light detection system from a particle irradiated by a light source in a flow stream, generating data signals in a plurality of photodetector channels in response to the detected light and binning data signals from two or more different photodetector channels to generate combined spectral data signals. Light detection systems having a detector component and a modulator component configured to bin data signals from two or more different photodetector channels are described. Systems (e.g., particle analyzer) having a light source and a light detection system for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.

A apparatus for detecting cells or particles in a fluid container includes a dispenser configured to dispense at least one cell or at least one particle into a defined sub-volume of a fluid with which the fluid container is at least partially filled, and a detection apparatus configured to, in a time-coordinated manner with dispensing the at least one cell or the at least one particle by the dispenser, perform a detection in the defined sub-volume and/or in one or several sub-volumes underneath the defined sub-volume in order to sense the at least one cell or the at least one particle when entering the fluid or immediately after entering the fluid.

The present invention relates to a device and method for high-throughput single cell stretching with the hydrodynamic force for assessing cellular mechanical properties. In an aspect of the invention, there is provided a uniquely designed microfluidic channel flowing with viscoelastic fluids, sensing electrodes integrated with the microchannel and a high-speed imaging and processing system. Cells are continuously pumped in the device, aligned and stretched. The arrival of individual cells prior to the cell stretching site can be detected by the electrical sensing unit, which produces a triggering signal to activate a high-speed camera for on-demand imaging of the cell motion and deformation. Cellular mechanical properties including cell size and cell deformability are extracted from the analysis of these captured single cell images.

In the present technology, the timing at which suction is performed is optimized in order to enhance the microparticle separation performance in a technology for separating target microparticles in a microchip. For this purpose, the present technology provides a method of optimizing a microparticle suction condition, and the like, using a microchip having a main flow channel through which a liquid containing a microparticle flows, a microparticle suction flow channel arranged coaxially with the main flow channel, and a branch flow channel branching from the main flow channel. The method includes: a branch point specifying process of specifying a branch point at which the branch flow channel branches from the main flow channel; and a time assignment process of assigning a time T.sub.1 to be applied to suction of the microparticle.

An imaging system for multiplex detection of biomolecules, including at least a first optical path and a second optical path; the first optical path is from an excitation light source to an objective lens, and a filter set is provided between the excitation light source and the objective lens and is configured to guide excitation light from the excitation light source to the objective lens; the second optical path is from the objective lens to an image acquisition device, a reflector is provided between the objective lens and the image acquisition device and is configured to project the fluorescence received by the objective lens to the image acquisition device; the excitation light source includes at least a first excitation light source used for excitation of fluorescent signals and a second excitation light source used for excitation of light-initiated chemiluminescence signals The system simultaneously realizes fluorescence and light-initiated chemiluminescence imaging.

An apparatus for nondestructive detection of transparent or reflective objects in a vessel includes an imager configured to acquire data that represent light reflected from spatial locations in the vessel as a function of time, a memory operably coupled to the imager and configured to store the data, and a processor operably coupled to the memory and configured to detect the objects based on the data by (i) identifying a respective maximum amount of reflected light, over time, for each location in the spatial locations based on the data representing light reflected from the spatial locations as a function of time, and (ii) determining a presence or absence of the objects in the vessel based on the number of spatial locations whose respective maximum amount of reflected light, over time, exceeds a predetermined value.

The present disclosure provides a technique for separating and identifying an abnormal cell in a cell sample derived from a subject. The present disclosure provides a method for analyzing cells using a cell analyzer by utilizing the functions, either alone or in combination, of the cell analyzer, said cell analyzer having a function of continuously concentrating cells, a function of successively arranging the cells in a specific region of a flow channel continuously, a function of simultaneously recognizing the shape of each cell, in a single cell unit on an image base, in the bright field and the shape of fluorescence, and a function of separating and purifying the cells having been recognized on the basis of the shape thereof obtained by correcting the aforesaid shape in accordance with the flow rate of the cells and the light emission data of the fluorescence.

Provided are a microparticle sorting device, and a method and a program for sorting microparticles capable of stabilizing sorting performance over a prolonged period of time.

The microparticle sorting device includes an imaging element and a controller. The imaging element obtains an image of fluid and fluid droplets at a position where the fluid discharged from an orifice which generates a fluid stream is converted into the fluid droplets. The controller controls driving voltage of an oscillation element which gives oscillation to the orifice and/or controls a position of the imaging element based on a state of the fluid in the image and/or a state of a satellite fluid droplet. The satellite fluid droplet does not include microparticles and exists between the position, where the fluid is converted into the fluid droplets, and a fluid droplet, among fluid droplets including the microparticles, which is closest to the position where the fluid is converted into the fluid droplets.