In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

An optical sensing and control device includes a light source emitting a light beam and an optical component in communication with the light beam. The optical component is configured to move the light beam in a plane. The plane extends into an area such that the light beam interacts with particles in the area producing a scattering of the light beam. The optical sensing and control device also includes a photodetector in communication with the particles within the plane. The photodetector configured to generate image data in response to the scattering of the light beam.

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

A system for dissociating cells from a cell culture vessel. The system comprises an imaging system configured to image a plurality of cells in a cell culture vessel being dissociated from at least one surface of the cell culture vessel by at least one cell dissociation agent; and at least one controller coupled to the imaging system and configured to: control the imaging system to capture a sequence of images of at least some cells in the plurality of cells during dissociation; and identify when to neutralize the at least one cell dissociation agent using the sequence of images.

An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the a fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid.

A system and process for testing and analyzing a laser beam is provided. The system and process allows for repeatable testing of a laser beam to provide a beam intensity profile while accounting for variation in measurements attributable to outside factors. The system and process further allows for simultaneous measurement and analysis of a laser beam having multiple wavelengths.

A system for modeling a collisional plasma particles distribution is provided. The system includes an input beam generator configured to generate an input beam having a first set of values of a parameter modelling an initial distribution of particles in a collisional plasma, a non-linear optical medium configured to receive the input beam and produce a complex response function in response to receiving the input beam, an output detector configured to detect a second set of values of the parameter responsive to propagation of the input beam through the non-linear medium to the output detector, a feedback module configured to modify one or more properties of the non-linear optical medium, and a controller configured to select the first set of values, receive the second set of values, and determine, based on the first set and the second set of values, a final distribution of the particles in the collisional plasma.

In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

A system for modeling a collisional plasma particles distribution is provided. The system includes an input beam generator configured to generate an input beam having a first set of values of a parameter modelling an initial distribution of particles in a collisional plasma, a non-linear optical medium configured to receive the input beam and produce a complex response function in response to receiving the input beam, an output detector configured to detect a second set of values of the parameter responsive to propagation of the input beam through the non-linear medium to the output detector, a feedback module configured to modify one or more properties of the non-linear optical medium, and a controller configured to select the first set of values, receive the second set of values, and determine, based on the first set and the second set of values, a final distribution of the particles in the collisional plasma.

A particle detection system is provided. The particle detection system comprises at least one transmitter; at least one receiver; a first interrogation volume formed by a first intersection of a first pair of a transmitter beam of a transmitter and a receiver field of view of a receiver; and a second interrogation volume formed by a second intersection of a second pair of a transmitter beam of a transmitter and a receiver field of view of a receiver.