Introduced here is an approach to improving the automatic identification of hematological diseases using computer-implemented models that are trained to rapidly distinguish between different collections of immunophenotypes that represent different disease types or disease states. Understanding the different patterns of immunophenotype collections contained in a given sample may permit a proposed diagnosis for a given hematological disease to be produced for the corresponding patient. For example, the proposed diagnoses may be output by a classification model based on the distribution of immunophenotypes across the given sample.

There is provided a technology that supports selection of a label to be used for analysis of target molecules. The present technology provides a label selection support system including an information acquisition unit that obtains, via a network, information associated with a plurality of target molecules to be analyzed, an information processor that obtains, using the information associated with a plurality of target molecules, in vivo expression information of the plurality of target molecules from a database storing in vivo expression information of target molecules and generates support information associated with assignment of a label to each of the plurality of target molecules on the basis of the expression information, and a transmitter that transmits the generated support information via the network.

A screening method is provided. Cells secreting target antibodies are screened by mixing candidate cells labeled with a first fluorescent molecule, a capture antigen and a labeled antibody against a target antibody and incubating, labeling using a high content cell imager and sorting using flow cytometry so as to screen cells secreting target antibodies. The screening method disclosed in the present application can automatically complete the labeling and sorting of target candidate cells in high throughput by labeling with a fluorescent molecule in combination with high-content cell imager and flow cytometer, so as to provide sufficient quantity of cells for subsequent amplification to obtain their antibody sequences and screen affinity antibodies. This method greatly improves the screening efficiency.

Embodiments of the present technology include a method for testing a blood sample for sepsis. The method may include receiving a blood sample from an individual. The method may also include executing an instruction to analyze the blood sample for sepsis. In addition, the method may include measuring values of a set of characteristics in the blood sample. The set of characteristics being determined prior to measuring the values. The method may further include analyzing the values of the set of characteristics to produce a representation of a suspicion of sepsis. In addition, the method may include displaying the representation. Embodiments also include systems for testing blood sample for sepsis.

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation.

The invention relates to a method for determining the absolute value of the flow velocity (v) of a particle-transporting medium. At least two measurement laser beams (L_i) with linearly independent, non-orthogonal measurement directions (b_i) are emitted. The measurement laser beams (L_i) scattered at particles are detected and one measurement signal (m_i) is generated in each case for each measurement laser beam (L_i). The measurement signals (m_i) are evaluated, wherein absolute values of velocity components (v_i) are ascertained as projections of the flow velocity (v) on the respective measurement directions (b_i), wherein a solid angle region is ascertained for the prevalent direction of the flow velocity (v) and signs assigned to this solid angle region are chosen for the individual velocity components (v_i), and wherein the absolute value of the flow velocity (v) is determined using the ascertained absolute values of the velocity components (v_i) and using the chosen signs for the velocity components (v_i).

Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.

A detection device for tiny particles in a liquid is provided. The detection device comprises includes a flow cell, a laser, a scattered light collection device, a photoelectric detector, a fiber Bragg grating and a first optical fiber coupler, wherein scattered light collected by the scattered light collection device is sent to the fiber Bragg grating through the first optical fiber coupler, and reflected light of the fiber Bragg grating after receiving the scattered light is sent to the photoelectric detector through the first optical fiber coupler. The device can eliminate most scattered light generated by the liquid, and reduce the interference of the scattered light of the liquid to scattered light signals generated by the particles, so that the scattered light signals captured by the photoelectric detector are mainly light signals generated by the particles.

An apparatus for sensing particulate matter in a fluid includes a fluid flow conduit fluidically connected to an interaction chamber; a light source positioned to illuminate the interaction chamber; and a light detector assembly positioned to receive light scattered by particulate matter present in the interaction chamber. The light detector assembly includes a light detector; and an optical element, the optical element configured to provide light to the light detector based on an incidence angle of the scattered light.

To provide a microchip that is easily handled.

Provided is a microchip having a plate shape and including: a sample liquid inlet into which a sample liquid is introduced; a main flow path through which the sample liquid introduced from the sample liquid inlet flows; and a sorting flow path into which a target sample is sorted from the sample liquid, in which the sample liquid inlet and a terminal end of the sorting flow path are formed on a same side surface. Furthermore, a sample sorting kit including the microchip is also provided. Moreover, a microparticle sorting device on which the microchip is mounted is also provided.