A device for observing a biological sample is provided, including: a light source to emit a light beam at a wavelength between 1 μm and 20 μm; an image sensor including pixels defining a detection plane; a holder to hold the sample between the source and the sensor at a distance from the plane smaller than 1 mm, such that the source is configured to illuminate an area of the sample larger than 1 mm.sup.2, no image-forming optics are placed between the sample and the sensor, and the sensor is configured to acquire an image corresponding to an area of the sample larger than 1 mm.sup.2 and representative of an absorption of the beam by the sample at the wavelength; and a processor to determine a map of an amount of analyte in the sample, based on the image acquired by the sensor, the analyte absorbing light at the wavelength.

To reduce a data amount. An information processing system according to an embodiment includes an excitation light source (100) that irradiates a respective plurality of samples belonging to a sample group with excitation light, a measurement unit (142) that measures fluorescence generated by irradiation of the samples with the excitation light, and an information processing unit (2) that generates differential data based on a difference between similar fluorescence signals among fluorescence signals based on the fluorescence measured for the respective samples.

The apparati, methods, and computer program products disclosed herein can be used to nondestructively detect undissolved particles, such as glass flakes and/or protein aggregates, in a fluid in a vessel, such as, but not limited to, a fluid that contains a drug.

A method serves for determining particles (3), in particular bacteria in fluid and operates using an imaging optical device with a light source (1), with an optical sensor (4) with a field of light-sensitive pixels and with a fluid sample, which is to be examined, arranged between the light source (1) and the sensor (4). Characteristics of at least one particle (3), which is detected with regard to imaging, are compared to characteristics of a characteristics collection for determining the detected particle (3). The image acquisition is effected with darkfield technology and a light-sensitive pixel comprises several subpixels which are used for image acquisition.

A seed imaging system for imaging seeds includes a seed transfer station configured to move seeds through the system. An imaging assembly includes a first camera mounted relative to the seed transfer station and configured to acquire images of the seeds as the seeds move through the system. A second camera is mounted relative to the seed transfer station and is configured to acquire images of the seeds as the seeds move through the system. The second camera has an imaging modality different from an imaging modality of the first camera. First and second cameras may be disposed above and below the seed transfer stations, such as a transparent belt.

In displaying a tomographic image in a slice in a three-dimensional (3D) medical image, for the purpose of achieving better recognition of a vascular structure contained in the slice without degrading spatial resolution of the slice, there is provided an image processing apparatus comprising: identifying section for identifying a slice of interest in a 3D medical image representing an anatomical part including a blood vessel; and projecting section for applying projection processing to pixel values in a slice axis direction of the slice for a region in the 3D medical image including the slice and wider than a slice width of the slice.

A method for identifying and enumerating candidate target cells within a biological fluid specimen is described. The method includes obtaining a biological fluid specimen, preparing the biological fluid specimen by staining cell features in the biological fluid specimen, capturing a digital image having a plurality of color channels of the biological fluid specimen, and applying image analysis to the digital image. A computer program product for identifying candidate target cells within a biological fluid specimen is also described. The computer program comprises instructions to cause a processor to carry out the image analysis.

The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

The inventive concept discloses a time series quantification method of supravital dye uptake of a cell using a lens-free imaging system.