The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.

Method for open-loop or closed-loop control of a process, in particular a downstream bioprocess, based on the projection of an unknown concentration of at least one substance in a sample using spectroscopy, in particular UV/vis spectroscopy, comprising the steps: Detect spectrums of a plurality of concentration samples, wherein at least two concentration samples have differing concentrations of the substance; generate several quantitative models based on the spectrums of the concentration samples, wherein the models each have a mapping of at least one spectral measurand of the spectrums to concentrations in concentration ranges, wherein the concentration ranges of two models are not identical; detect at least one sample spectrum of the sample; map the sample spectrum to at least one quantitative model of the generated quantitative models; apply the at least one quantitative model that was mapped to the sample spectrum against the sample spectrum to determine a projected value for the unknown concentration; and apply open-loop and/or closed-loop control of the process for at least one parameter based on the projected value for the undetermined concentration.

A fluorescence photometer includes a photometer unit and an optical fiber unit. The photometer unit includes a light source, an excitation-side spectroscope for separating light emitted from the light source to generate excitation light, and a fluorescence-side spectroscope for separating fluorescent light emitted from a sample irradiated with the excitation light to generate monochromatic light. The optical fiber unit guides the excitation light to the sample placed outside the photometer unit and guides the fluorescent light emitted from the sample to the photometer unit and includes an image fiber for capturing an image of the sample, an excitation-side fiber arranged around the image fiber and for guiding the excitation light to the sample, and a fluorescence-side fiber arranged around the image fiber and to guide the fluorescent light emitted from the sample to the photometer unit. The excitation-side fiber and the fluorescence-side fiber are arranged to surround the image fiber.

The invention relates to a device for a light-spectroscopic analysis of a, for example, liquid sample. In particular, light should be guided through a sample and then detected and/or analyzed photometrically, spectrophotometrically, fluorometrically, spectrofluorometrically and/or by means of phosphorescence or luminescence.

Disclosed is a method for diagnosing a neurodegenerative disease in a subject. The method comprises obtaining from the subject a sample comprising at least one live blood cell, and optionally isolating at least one live blood cell from the sample. The method further comprises generating one or more multispectral or hyperspectral images of the at least one cell, and analysing spectral characteristics of autofluorescence from the at least one cell. Also disclosed is a system configured to aid in the detection or diagnosis of a neurodegenerative disease. Also disclosed is a method for selecting a subject for treatment for a neurodegenerative disease. Also disclosed is a method for monitoring the response of a subject to a therapeutic treatment for a neurodegenerative disease. Also disclosed is a protocol for monitoring the efficacy of a therapeutic treatment for a neurodegenerative disease.

Systems and methods are provided for multi-spectral or hyper-spectral fluorescence imaging. In one example, a spectral encoding device may be positioned in a detection light path between a detection objective and an imaging sensor of a microscope. In one example, the spectral encoding device includes a first dichroic mirror having a sine transmittance profile and a second dichroic mirror having a cosine transmittance profile. In addition to collecting transmitted light, reflected light from each dichroic mirror is collected and used for total intensity normalization and image analysis.

A system and method for high resolution multi-fluorescence imaging with synchronized image acquisition amongst sensors can be used to simultaneously capture fluorescence signals from multiple fluorophores over extremely large fields of view. The system can include an array of micro-cameras, along with a particular arrangement of fluorescent filters that can be fixed in one location or moved to new locations.

A resin composition quality controlling method includes a step of measuring a Raman spectrum of a resin composition composed of TiO.sub.2 particles dispersed in a base material mainly composed of a silicone rubber by irradiating the resin composition with laser, and a step of determining a concentration of the TiO.sub.2 particles in the resin composition based on an intensity of a fluorescence spectrum in the Raman spectrum.

The present disclosure relates to a method and device for unmixing fluorescent signals based on a joint histogram. The method and device may be configured to obtain a plurality of images of a plurality of fluorescent materials marked in different biomolecules and to unmix the signals of the fluorescent materials based on at least one joint histogram index defined between the images of each of pairs each including two or more of the obtained images. According to the present disclosure, the joint histogram index may include at least one of Kullback-Leibler divergence, cross-entropy, or Rand-index.

A detector device for the remote analysis of materials, in particular hazardous materials, including at least one laser, which is designed to emit pulsed laser light onto a sample located at a detection distance, and a telescope, which is designed to collect and/or focus laser light scattered on the sample and to forward the scattered laser light into an optical spectrometer. The optical spectrometer is designed for a spectral analysis of the laser light scattered on the sample. The laser is followed by a first beam path with a first reference beam and an additional beam path with a second reference beam for the scattered laser light. A unit is provided for determining a time difference between pulses of the first reference beam and pulses of the second reference beam, wherein the detection distance can be determined from the time difference. The unit is designed to determine the detection distance in real-time.